A functional cellular framework for sex and estrous cycle-dependent gene expression and behavior.

Sex hormones exert a profound influence on gendered behaviors. How individual sex hormone-responsive neuronal populations regulate diverse sex-typical behaviors is unclear. We performed orthogonal, genetically targeted sequencing of four estrogen receptor 1-expressing (Esr1+) populations and identified 1,415 genes expressed differentially between sexes or estrous states. Unique subsets of these genes were distributed across all 137 transcriptomically defined Esr1+ cell types, including estrous stage-specific ones, that comprise the four populations. We used differentially expressed genes labeling single Esr1+ cell types as entry points to functionally characterize two such cell types, BNSTprTac1/Esr1 and VMHvlCckar/Esr1. We observed that these two cell types, but not the other Esr1+ cell types in these populations, are essential for sex recognition in males and mating in females, respectively. Furthermore, VMHvlCckar/Esr1 cell type projections are distinct from those of other VMHvlEsr1 cell types. Together, projection and functional specialization of dimorphic cell types enables sex hormone-responsive populations to regulate diverse social behaviors.

An accessory prefrontal cortex-thalamus circuit sculpts maternal behavior in virgin female mice.

The ability to care for the young is innate and readily displayed by postpartum females after delivery to ensure offspring survival. Upon pup exposure, rodent virgin (nulliparous) females also develop parental behavior that over time becomes displayed at levels equivalent to parenting mothers. Although maternal behavior in postpartum females and the associated neurocircuits are well characterized, the neural mechanisms underlying the acquisition of maternal behavior without prior experience remain poorly understood. Here, we show that the development of maternal care behavior in response to first-time pup exposure in virgin females is initiated by the activation of the anterior cingulate cortex (ACC). ACC activity is dependent on feedback excitation by Vglut2+ /Galanin+ neurons of the centrolateral nucleus of the thalamus (CL), with their activity sufficient to display parenting behaviors. Accordingly, acute bidirectional chemogenetic manipulation of neuronal activity in the ACC facilitates or impairs the attainment of maternal behavior, exclusively in virgin females. These results reveal an ACC-CL neurocircuit as an accessory loop in virgin females for the initiation of maternal care upon first-time exposure to pups.

Selective Deletion of Methyl CpG Binding Protein 2 from Parvalbumin Interneurons in the Auditory Cortex Delays the Onset of Maternal Retrieval in Mice.

Mutations in MECP2 cause the neurodevelopmental disorder Rett syndrome. MECP2 codes for methyl CpG binding protein 2 (MECP2), a transcriptional regulator that activates genetic programs for experience-dependent plasticity. Many neural and behavioral symptoms of Rett syndrome may result from dysregulated timing and thresholds for plasticity. As a model of adult plasticity, we examine changes to auditory cortex inhibitory circuits in female mice when they are first exposed to pups; this plasticity facilitates behavioral responses to pups emitting distress calls. Brainwide deletion of Mecp2 alters expression of markers associated with GABAergic parvalbumin interneurons (PVins) and impairs the emergence of pup retrieval. We hypothesized that loss of Mecp2 in PVins disproportionately contributes to the phenotype. Here, we find that deletion of Mecp2 from PVins delayed the onset of maternal retrieval behavior and recapitulated the major molecular and neurophysiological features of brainwide deletion of Mecp2 We observed that when PVin-selective mutants were exposed to pups, auditory cortical expression of PVin markers increased relative to that in wild-type littermates. PVin-specific mutants also failed to show the inhibitory auditory cortex plasticity seen in wild-type mice on exposure to pups and their vocalizations. Finally, using an intersectional viral genetic strategy, we demonstrate that postdevelopmental loss of Mecp2 in PVins of the auditory cortex is sufficient to delay onset of maternal retrieval. Our results support a model in which PVins play a central role in adult cortical plasticity and may be particularly impaired by loss of Mecp2 SIGNIFICANCE STATEMENT Rett syndrome is a neurodevelopmental disorder that includes deficits in both communication and the ability to update brain connections and activity during learning (plasticity). This condition is caused by mutations in the gene MECP2 We use a maternal behavioral test in mice requiring both vocal perception and neural plasticity to probe the role of Mecp2 in social and sensory learning. Mecp2 is normally active in all brain cells, but here we remove it from a specific population (parvalbumin neurons). We find that this is sufficient to delay learned behavioral responses to pups and recreates many deficits seen in whole-brain Mecp2 deletion. Our findings suggest that parvalbumin neurons specifically are central to the consequences of loss of Mecp2 activity and yield clues as to possible mechanisms by which Rett syndrome impairs brain function.

Postnatal rearing environment alters pup cues for caregiver-offspring interactions.

Maternal behavior experienced in early life provides essential scaffolding to infant psychobiology with life-long effects on neurobiological and behavioral outcomes. However, infants are not passive recipients of caregiving. Evidence in rodents suggests that pups actively contribute to dam-pup interactions by soliciting maternal care with auditory, tactile, and hormonal cues. The limited bedding and nesting material (LBN) rearing manipulation induces changes in maternal care that have been attributed to maternal stress caused by the low-resource environment. The goal of the current study was to determine whether LBN also alters pup cues for maternal behavior, with implications for the mechanism of LBN-induced effects. Rat dams and pups were randomly assigned to LBN or Control rearing conditions on postnatal day (P) 0-6 and pups were fostered to the same or different condition on P6-13. LBN increased pup-directed maternal behaviors measured through 24 h monitoring using machine learning based automated analysis. LBN altered several pup cues known to affect maternal behavior including reducing pup core body temperature, reducing body weight, and altering pup vocalizations on P6 and P12. P6-13 LBN-exposed pups had elevated serum testosterone, which positively correlated with maternal licking and grooming. LBN reduced pup movement between nest attendance onset and the start of nursing, which was negatively related to dam nursing latency and contributed to longer nursing latency in LBN dams. P0-6 pup exposure to LBN also led to longer nest attendance bouts and shorter licking and grooming bouts on P7 and P9, suggesting lasting effects of LBN on pups. These data demonstrate that LBN changes pup behavioral and hormonal signals consistent with eliciting more maternal care, contributing to augmented pup-directed behaviors. This bidirectional interplay may be a critical mechanism involved in the lasting effects of early life environments.

Effects of intrauterine position during gestation on specific endocrine and behavioral parameters that impact reproduction in domestic rabbits.

Prior studies from others, performed in a different breed, reported that doe rabbits developing between two male siblings (2 M) during gestation display characteristics indicative of masculinization: larger anogenital distance (AGD), larger submandibular glands, and higher chinning frequency than females with zero (0 M) or one (1 M) contiguous brothers. Similar effects are provoked by injecting androgens to the pregnant doe suggesting that prenatal androgen exposure masculinizes female embryos. To further understand the scope of such masculinization we compared 0 M, 1 M, and 2 M females regarding behavioral, neuroendocrine, and somatic parameters, related or not to reproduction. IUP did not impact: body weight, sexual receptivity, mating-induced LH secretion, maternal nest-building, litter size, or milk output. At puberty: a) chinning frequency was: 0 M and males>1 M and 2 M; b) ambulation in open field was lowest in 1 M females and males. IUP effects on AGD were significant only on postnatal day 1: 0 M, 1 M, and males>2 M, in contrast to earlier study. Willingness to nurse at delivery was less frequent in 2 M than in 1 M and 0 M does and correlated with nursing occurrence across lactation. Does that did not nurse at parturition delivered fewer kits/min than those that nursed then, regardless of IUP. The duration of nursing bouts across lactation was significantly longer in the1 M and 2 M does that showed this behavior on postpartum days 1-20. Our findings indicate that IUP is associated with alterations in specific aspects of postpartum maternal behavior.

Maternal rejection but not protectiveness predicts juvenile Japanese macaque behavior without direct maternal influence.

Primates show large interindividual variability in the character and quantity of interactions between mothers and their immature offspring. Multiple studies have documented associations between maternal behavior and the occurrence or frequency of certain behaviors among offspring, but it remains unclear whether and how early maternal interactions generally affect behavioral development in offspring. We followed two wild groups of Japanese macaques on Yakushima island and investigated the relationship between maternal behavior and several types of behavior performed by 35 juvenile offspring. We further asked if the impact of maternal behavior on juvenile behavior persists regardless of the distance between mother and offspring, testing whether the influence extends beyond cases when the mother is nearby. We found that juveniles whose mothers frequently rejected them approached and played with others more often, independent of their mother's presence. Juveniles of more protective mothers were in proximity to fewer other individuals and played less, but only if their mothers were nearby. Maternal rejection appears to exert a generalized effect on offspring behavior that endures when mothers are absent. In contrast, effects of maternal protectiveness may be temporary and/or reflect direct maternal influences, such as active intervention in offspring interactions, or effects of the mother's own social relationships on offspring interactions. Our results suggest that understanding how maternal behavior affects offspring development requires paying attention to the context of juvenile behavior, including the mother's distance from her offspring.

Maternal behavior of dairy cows and suckling behavior of dairy calves in different cow-calf contact conditions.

Cow-calf contact has been suggested as an alternative to the common practice of early separation of dairy cow and calf. Increasing cow-calf contact poses challenges by reducing the machine milk yield and increasing separation stress. Part-time cow-calf contact may prove more feasible than full-time contact for farmers but may affect the maternal behavior of the cows. Gradually reducing the daily contact could gradually wean the calf off milk, preparing it for separation. This study aims to investigate (1) how cows' maternal behaviors toward their own calf are affected by part-time contact, and (2) whether calves' suckling behavior is reduced by reducing daily cow contact. Fifty-six dairy cows and their calves were assigned to either full-time (23 h/d) or part-time (10 h/d) cow-calf contact for 7 wk (main rearing period). This was followed by 2 wk of either reduced contact (50% in wk 8, then 25% of original contact time in wk 9) or unchanged contact. First, the maternal behavior of full- and part-time cows was observed for 24 h in the third week of the main rearing period. Part-time cows spent less time nursing and grooming their own calf than full-time cows did. However, the amount of nursing in the inverse parallel position and the probability of a cow nursing a calf other than her own did not differ between the 2 treatments. Second, calf suckling behavior was observed for 24 h in both weeks of the reduced contact period. Averaged across both observations, total suckling time (i.e., time spent suckling any cow) was lower in part-time calves that experienced reduced contact compared with part-time calves with unchanged contact. In wk 9, time spent suckling only the dam was lower for full-time calves with reduced contact compared with part-time calves with unchanged contact. In conclusion, although we found a lower nursing and grooming time in part-time cows compared with full-time, we found no difference between treatments with regard to nursing in the inverse parallel position and nursing a calf other than the cow's own. The effect of gradually reducing cow-calf contact on calves' suckling time requires more research.

Assessing the emotional states of dairy cows housed with or without their calves.

The practice of keeping dairy cows with their calves continues to gain interest. Cow-calf contact, or lack thereof, is expected to affect emotional states, but this requires empirical testing. Different types of cow-calf contact may also affect the emotional states of cows. The primary objective of this study was to assess the emotional state of dairy cows with full-time (23 h/d), part-time (10 h/d), or no-contact with their calves (separated 48 h after birth), using a visual judgment bias test (JBT) about one month after calving; JBT is the current gold-standard method to assess emotional state in animals by evaluating optimism or pessimism (illustrated by the proverbial half-full or half-empty water glass). The secondary objective was to compare outcomes of color- versus shape-based visual JBT. Fifty dairy cows were trained to approach a positive image on a screen (rewarded with food) and to avoid a negative image (else punished with waving bag). Once learned (>80% correct over 2 consecutive days), cows were presented with 3 ambiguous images (each presented once per day among 4 positive and 3 negative images, repeated over 4 d), and their approach responses recorded. For the color method (10 full-time, 9 part-time and 11 no-contact cows), positive and negative images were a solid red or white background; ambiguous images were shades of pink. For the shape method (8 full-time, 6 part-time and 6 no-contact cows), positive and negative images were a white circle or cross on a black background; ambiguous images were overlaid circle and cross in varying shades of gray. Cows learned to discriminate colors quicker than shapes (7.3 d, confidence limits [CL]: 6.6-8.2 d; vs. 9.3 d, CL: 8.1-10.6 d). Approaches to ambiguous colors followed a generalization curve (81.0, 33.1, and 5.0 ± 3.7% for near-positive, middle, and near-negative images, respectively), but not approaches to ambiguous shapes (31.9%, 25.7%, and 21.9% ± 4.8%, respectively), indicating colors over shapes should be used in visual JBT for cattle. Part-time cows approached fewer ambiguous color images than full-time cows (23.5%, CL: 13.4%-34.4%; vs. 44.8%, CL; 32.8%-57.1%) whereas no-contact cows were intermediate, but not different from full-time or part-time cows (37.8%, CL; 26.8%-49.5%). The color JBT results show a pessimistic bias (indicating a negative emotional state) in cows with part-time calf contact, possibly due to repeated daily separation from her young calf, relative to cows with full-time calf contact. Thus, cow-calf contact systems appear to influence the emotional state of cows depending on the practice. Cows without calf contact showed no difference in judgment bias between cows with full- or part-time calf contact, suggesting these cows probably do not experience a pervasive negative emotional state (relative to those with calf contact) approximately 30 d after calf separation. However, individual variability in judgment bias was evident for all treatments. The visual judgment bias test is a useful methodology for assessing emotional states of dairy cows; future research should prioritize understanding the emotional states of dairy cows in alternative management systems.

Do toys get in the way? The duration of shared emotional experiences is longer when mothers engage their infants without toys.

During mother-infant interaction, shared emotional experiences, defined as reciprocal and synchronous emotional sharing between mother and infant, are an indicator of early relational health. Yet, it is unclear how mothers' efforts to engage with their infants relate to dyadic-level shared emotional experiences. Utilizing a sample of 80 randomly selected videos of the NICHD Study of Early Child Care and Youth Development, we examined how mothers' bids for interaction with their 6-month-old infants related to the duration of shared emotional experiences. An event sampling, sequential coding system was used to identify a maternal bid for interaction (i.e., with toy, without toy) and the subsequent presence or absence of a shared emotional experience, including duration of the shared emotional experience. Results indicated that shared emotional experiences were longer following mothers' efforts to engage their infants in play without toys. Findings suggest that methods matter; researchers and practitioners interested in studying and promoting shared emotional experiences between mothers and infants may wish to focus on dyadic interactions without toys.

Durante la interacción madre-infante, las experiencias emocionalmente compartidas, definidas como el recíproco y síncrono compartir emocional entre madre e infante, son un indicador de la temprana saludable relación. Aún así, no está claro cómo los esfuerzos de las madres para compartir con sus infantes se relacionan con las experiencias emocionales compartidas al nivel de la díada. Utilizando un grupo muestra de 80 videos del Estudio NICHD del Temprano Cuidado Infantil y Desarrollo de la Juventud, seleccionados al azar, examinamos cómo las posturas de las madres para interactuar con sus infantes de 6 meses de edad se relacionaban con la duración de las experiencias emocionales compartidas. Se usó un sistema de codificación secuencial de muestreo de eventos para identificar una postura materna para la interacción (v.g., con juguete, sin juguete) y la subsecuente presencia o ausencia de una experiencia emocional compartida, incluyendo la duración de la experiencia emocional compartida. Los resultados indicaron que las experiencias emocionales compartidas eran más largas cuando los esfuerzos de las madres para interactuar con sus infantes en el juego no incluían juguetes. Los resultados sugieren que los métodos importan; los investigadores y profesionales de la práctica interesados en estudiar y promover las experiencias emocionales compartidas entre madres e infantes pudieran querer enfocarse en las interacciones diádicas sin juguetes.

Precise and Pervasive Phasic Bursting in Locus Coeruleus during Maternal Behavior in Mice.

The noradrenergic locus coeruleus (LC) mediates key aspects of arousal, memory, and cognition in structured tasks, but its contribution to naturalistic behavior remains unclear. LC activity is thought to multiplex distinct signals by superimposing sustained ("tonic") firing patterns reflecting global brain states, such as arousal and anxiety, and rapidly fluctuating ("phasic") bursts signaling discrete behaviorally significant events. Manipulations of the LC noradrenergic system broadly impair social behavior, but the temporal structure of LC firing and its relationship to social interaction is unknown. One possibility is that tonic firing may increase in the presence of social partners; it is also possible that phasic bursts may accompany specific social events. We used chronic in vivo electrophysiology and fiber photometry to measure single-unit and population neural activity in LC of freely behaving mice during their interactions with pups. We find that pup retrieval elicits remarkably precise phasic activity in LC that cannot be attributed merely to sensory stimuli, motor activity, or reward. Correlation of LC activity with retrieval events shows that phasic events are most closely related to specific subsequent behaviors. The reliability and magnitude of phasic responses strongly suggest that these events are coordinated across LC and broadcast noradrenaline (NA) release throughout the brain. We also observed slow changes in tonic firing when females performed distinct maternal behaviors such as nest building and pup grooming. We therefore propose that LC signals state changes during sustained interactions and contributes to goal-directed action selection during social behavior with globally broadcast NA release.SIGNIFICANCE STATEMENT Locus coeruleus (LC) releases noradrenaline (NA) brain wide, influencing many cognitive, emotional, and physiological processes. Multifunctionality of LC is maintained by multiplexing NA signaling via brief "phasic" patterns of bursting and slowly changing "tonic" firing. Manipulations of NA impair social behavior, yet the structure of LC activity with respect to specific social events is unknown. We measured LC activity in mice freely interacting with pups. We find that pup retrieval elicits precisely timed and pervasive phasic activation of LC that anticipates specific behaviors. We also found that LC neurons exhibited slow fluctuations in firing during sustained behaviors. We propose that LC simultaneously contributes to goal-directed social action selection with globally broadcast NA release and signals social state changes with increased tonic firing.

Wild-type MECP2 expression coincides with age-dependent sensory phenotypes in a female mouse model for Rett syndrome.

Rett syndrome is characterized by an early period of typical development and then, regression of learned motor and speech skills in girls. Loss of MECP2 protein is thought to cause Rett syndrome phenotypes. The specific underlying mechanisms from typical developmental trajectory to regression features throughout life are unclear. Lack of established timelines to study the molecular, cellular, and behavioral features of regression in female mouse models is a major contributing factor. Due to random X-chromosome inactivation, female patients with Rett syndrome and female mouse models for Rett syndrome (Mecp2Heterozygous , Het) express a functional copy of wild-type MECP2 protein in approximately half of all cells. As MECP2 expression is regulated during early postnatal development and experience, we characterized the expression of wild-type MECP2 in the primary somatosensory cortex of female Het mice. Here, we report increased MECP2 levels in non-parvalbumin-positive neurons of 6-week-old adolescent Het relative to age-matched wild-type controls, while also displaying typical levels of perineuronal net expression in the barrel field subregion of the primary somatosensory cortex, mild tactile sensory perception deficits, and efficient pup retrieval behavior. In contrast, 12-week-old adult Het express MECP2 at levels similar to age-matched wild-type mice, show increased perineuronal net expression in the cortex, and display significant tactile sensory perception deficits. Thus, we have identified a set of behavioral metrics and the cellular substrates to study regression during a specific time in the female Het mouse model, which coincides with changes in wild-type MECP2 expression. We speculate that the precocious increase in MECP2 expression within specific cell types of adolescent Het may provide compensatory benefits at the behavioral level, while the inability to further increase MECP2 levels leads to regressive behavioral phenotypes over time.

Predicting childhood ADHD-linked symptoms from prenatal and perinatal data in the ABCD cohort.

This study investigates the capacity of pre/perinatal factors to predict attention-deficit/hyperactivity disorder (ADHD) symptoms in childhood. It also explores whether predictive accuracy of a pre/perinatal model varies for different groups in the population. We used the ABCD (Adolescent Brain Cognitive Development) cohort from the United States (N = 9975). Pre/perinatal information and the Child Behavior Checklist were reported by the parent when the child was aged 9-10. Forty variables which are generally known by birth were input as potential predictors including maternal substance-use, obstetric complications and child demographics. Elastic net regression with 5-fold validation was performed, and subsequently stratified by sex, race/ethnicity, household income and parental psychopathology. Seventeen pre/perinatal variables were identified as robust predictors of ADHD symptoms in this cohort. The model explained just 8.13% of the variance in ADHD symptoms on average (95% CI = 5.6%-11.5%). Predictive accuracy of the model varied significantly by subgroup, particularly across income groups, and several pre/perinatal factors appeared to be sex-specific. Results suggest we may be able to predict childhood ADHD symptoms with modest accuracy from birth. This study needs to be replicated using prospectively measured pre/perinatal data.

Human milk expression in the first year postpartum among persons with low incomes.

BACKGROUND: Expressing milk (i.e., human milk) is common in the USA, but practices are unknown among families in the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC). This study of a WIC population explores the practice of and reasons for expressing milk in the first year postpartum. METHODS: We analysed data from a longitudinal study to examine milk expression at 1, 3, 5, 7, 9, 11, and 13 months postpartum among breastfeeding persons enrolled in WIC with term singletons. We cross-sectionally analysed the weighted prevalence of milk expression at each survey month and report reasons for milk expression in the first 7 months. RESULTS: Among the study participants who reported feeding human milk at Month 1, 70.4% expressed milk in the first 13 months postpartum. The prevalence of milk expression was 56.8% at Month 1 and decreased to 13.9% at Month 13 among those feeding any human milk that month. Reasons for expressing milk changed over time; in the first month, increasing milk supply, relieving engorgement, and having an emergency supply of milk were common. In later months, having a supply of milk available so that someone else could feed their infant was common. CONCLUSIONS: Clinicians, health educators, WIC staff, and others working with WIC families can promote optimal expressed milk feeding and storage practices. Extra attention and support may be especially important in the first months postpartum when milk expression is common. Support for persons who are expressing milk can be tailored for reasons of milk expression.

ADSA Foundation Scholar Award: What makes for a good life for transition dairy cows? Current research and future directions.

Dairy cows experience several challenges during the transition period, historically defined as the 3 wk before to 3 wk after calving. During this 6-wk window, cows undergo a series of social, nutritional, and physical changes that affect their quality of life. Cows are also at the highest risk of becoming ill in the days and weeks after calving compared with any other period in their adult life. Because of this, the transition cow has been a central focus of dairy cattle research for at least the last 50 yr, with much of this work targeted at identifying, treating, and preventing postpartum disease. However, understanding what makes for a good life for transition cows requires consideration of more than just their health. When considering a cow's welfare, we must also include her emotional experiences and ability to live a reasonably natural life. To gain a broader perspective on the welfare of transition cows that goes beyond their health, continued inter- and transdisciplinary approaches are needed. The aims of this narrative review are to (1) describe a framework used to study animal welfare, which includes different perspectives on what makes for a good life for animals using examples from transition cow research, (2) summarize the advancements we have made in developing our understanding of the welfare of transition dairy cows over the last several decades, (3) identify gaps in the literature and propose new and continued topics for research, and (4) suggest a path forward for researchers, including the use of methods from both the natural and social sciences to rethink existing problems, understanding barriers to adoption of evidence-based practice, and prepare for future challenges.

Anxiety, aggression, reward sensitivity, and forebrain dopamine receptor expression in a laboratory rat model of early-life disadvantage.

Despite early-life disadvantage (ELD) in humans being a highly heterogenous construct, it consistently predicts negative neurobehavioral outcomes. The numerous environmental contributors and neural mechanisms underlying ELD remain unclear, though. We used a laboratory rat model to evaluate the effects of limited resources and/or heavy metal exposure on mothers and their adult male and female offspring. Dams and litters were chronically exposed to restricted (1-cm deep) or ample (4-cm deep) home cage bedding postpartum, with or without lead acetate (0.1%) in their drinking water from insemination through 1-week postweaning. Restricted-bedding mothers showed more pup-directed behaviors and behavioral fragmentation, while lead-exposed mothers showed more nestbuilding. Restricted bedding-raised male offspring showed higher anxiety and aggression. Either restricted bedding or lead exposure impaired goal-directed performance in a reinforcer devaluation task in females, whereas restricted bedding alone disrupted it in males. Lead exposure, but not limited bedding, also reduced sucrose reward sensitivity in a progressive ratio task in females. D1 and D2 receptor mRNA in the medial prefrontal cortex and nucleus accumbens (NAc) were each affected by the early-life treatments and differently between the sexes. Most notably, adult males (but not females) exposed to both early-life treatments had greatly increased D1 receptor mRNA in the NAc core. These results illuminate neural mechanisms through which ELD threatens neurobehavioral development and highlight forebrain dopamine as a factor.

Behavior of calves reared with half-day contact with their dams.

Dam-calf contact has been suggested to improve animal welfare in dairy calves, but practical and economical concerns have led to interest in half-day contact between cows and their calves. However, little is known about the behavioral effects of half-day contact compared with whole-day contact. The present study investigated the behavior of 45 dairy calves housed with their dams in either a whole-day system (cows only away twice daily for milking) or a half-day system (cows away from afternoon milking until after morning milking the next day). Data were video recorded during 24 h when calves were on average 3, 5, and 7 wk old. Half-day calves spent less time suckling and received less grooming compared with whole-day calves, indicating that they received less maternal care. Half-day calves were quicker to reunite with their dam when the cows returned from morning milking compared with whole-day calves. Half-day calves also suckled alien cows more often, although mainly shortly after the cows returned to the pen in the morning. This may indicate that they were hungrier than whole-day calves at this time of day. Half-day calves spent more time eating solid feeds, which may prepare them better for separation from the dam and weaning off milk. In conclusion, half-day dam-calf contact may affect calf welfare both positively and negatively, and further research focusing more directly on assessing affective states is encouraged.

Comparing weaning methods in dairy calves with different dam contact levels.

Allowing the dam to rear her calf is an alternative practice in the dairy industry where cow and calf may gain welfare benefits from performing natural and highly motivated behaviors. However, this system has been linked to an increased separation and weaning response. Reducing the daily dam-calf contact time may be a way to prepare the calf for weaning and separation. The first aim of the present study was to investigate the effect of 8 wk of half-day dam-calf contact on calf response to weaning and separation, compared with calves reared with whole-day dam-calf contact and an artificially reared, group-housed control with unrestricted access to milk for 20 min twice daily. Weaning off milk and separation from the dam can be viewed as 2 independent stressors. By introducing each stressor separately, it may be possible to reduce the overall behavioral response. The second aim of the present study was to investigate the effect of 1-wk fence-line weaning before permanent separation. The study was conducted with a 3 × 2 factorial design with dam contact treatments (whole-day, half-day, and control) and weaning treatments (simultaneous, and stepwise). Whole-day calves were separated twice daily from their dams during milking, whereas half-day calves were separated daily from the afternoon milking and until next morning milking. Simultaneous weaning and separation were done in wk 9, and stepwise weaning and separation started in wk 8 with calves being fence-line weaned before permanent separation in wk 9. Data were collected on 69 dairy calves in wk 8 and wk 9, and data were summarized over the 2 weeks for analysis. Stepwise weaning and separation reduced the number of high-pitched vocalizations and activity of dam-reared dairy calves but had little effect on control calves. There was no difference between whole-day and half-day calves in their response to separation, but as expected, dam-reared calves reacted more strongly than the control group. This was also reflected in the average daily BW gain the week after weaning, with control calves having higher average daily gains than whole-day, while half-day calves were intermediate. However, the behavioral response did not fully wane within the observation period (0-48 h of interventions). In conclusion, 1-wk fence-line weaning reduced the summed weaning and separation response in dam-reared calves. However, no difference between half-day dam-calf contact and whole-day dam contact was detected in the behavioral response to weaning and separation.

Prevalence and correlates of child maltreatment among mothers in the Kurdistan province, Western Iran.

OBJECTIVE: Parental child maltreatment is a serious public health issue worldwide. As mothers take on a significant proportion of parenting duties in two-parent families, knowledge of maternal risk factors for child maltreatment is essential. METHODS: In this cross-sectional study, 135 mothers with their last child under 18 years in Kurdistan province were enrolled. A validated Persian version of the ISPCAN Child Abuse Screening Tool-Parent version, Beck Anxiety Inventory, and Beck Depression Inventory were administered. RESULTS: The prevalence of severe and moderate physical punishment was 78.5% and 71.9%, respectively. Psychological punishment was reported by 99.3% of respondents, and 48.9% reported neglect. A relationship was found between child physical and emotional abuse and low maternal education (p < 0.01), domestic violence (p = 0.02), maternal exposure to maltreatment during childhood (p = 0.03), maternal depression (p < 0.01), and maternal anxiety (p < 0.01). A relationship was also found between child neglect and residence in rural areas (p < 0.01), domestic violence, and low maternal education (p = 0.02). CONCLUSION: Maternal child maltreatment in Iran is increased in mothers with psychological disorders and those with certain demographic characteristics. Clinicians should be alert to these potential risk factors.

Weathering the storm alone or together: Examining the impact of COVID-19 on sole and partnered working mothers.

Sole employed mothers and their families face numerous challenges. Yet, the unprecedented circumstances of the COVID-19 pandemic may be adding additional risk to the already precarious day-to-day reality of this population. Thus, we examine the implications of this crisis for the mental health and job-related well-being of both sole and partnered working mothers. Participants were 206 mothers who continued to work during the pandemic. A moderated mediation model was analyzed. Work-family conflict (WFC) during the pandemic differentially related to mothers' parenting stress, based on romantic partnership status; when mothers were sole parents, the relationship between WFC and parenting stress was exacerbated. Moreover, this stress mediated the relationship between WFC and both poor mental health and decreased work engagement for sole employed mothers. Findings broaden our understanding of the implications of the COVID-19 pandemic for sole and partnered employed mothers, and how this crisis may be increasing disparities between working sole-parent and dual-partner families.

Disrupted maternal communication and disorganized attachment in the Arab society in Israel.

Disrupted maternal communication during mother-infant interaction has been found to be associated with infants' disorganized attachment, but has been studied primarily in North American and European samples and not in Arab samples. To address this gap the study examined the association between disrupted maternal communication and infant attachment in a sample of 50 Arab mothers and their one-year-old infants in Israel. Attachment was assessed with the Strange Situation Procedure (SSP), and disrupted communication with the AMBIANCE. Disrupted communication was higher in mothers of infants with disorganized and ambivalent attachment than in mothers of securely attached infants. The findings support the link between disrupted communication and disorganized attachment in the Arab society in Israel and add to our understanding of maternal behavior associated with ambivalent attachment.

Se ha determinado que la interrumpida comunicación materna durante la interacción madre-infante está asociada con la desorganizada afectividad de los infantes, pero ha sido estudiada primariamente en grupos muestras norteamericanos y europeos, no así en grupos muestras árabes. Con el fin de ocuparse de este vacío, el estudio examinó la asociación entre la interrumpida comunicación materna y la afectividad del infante en un grupo muestra de 50 madres árabes y sus infantes de un año de edad en Israel. Se evaluó la afectividad con el Procedimiento de la Situación Extraña (SSP), y la interrumpida comunicación con AMBIANCE (Lyons-Ruth et al., 1999). La interrumpida comunicación fu mayor en madres de infantes con afectividad desorganizada y ambivalente que en madres de infantes afectivamente seguros. Los resultados apoyan la conexión entre la interrumpida comunicación y la desorganizada afectividad en la sociedad árabe en Israel y también son un aporte a nuestra comprensión del comportamiento materno asociado con la afectividad ambivalente.

On sait que la communication maternelle perturbée durant l'interaction mère-bébé est liée à l'attachement désorganisé des bébés, mais cela a été avant tout étudié chez des échantillons d'Amérique du Nord et européens, et non chez des échantillons arabes. Afin de combler ce fossé l'étude a examiné le lien entre la communication maternelle perturbée et l'attachement du bébé chez un échantillon de 50 mères arabes et leurs bébés d'un an en Israël. L'attachement a été évalué avec la Procédure de Situation Etrange (SSP en anglais) et la communication perturbée a été évalué avec l'AMBIANCE (Lyons-Ruth et al., 1999). La communication perturbée était plus élevée chez les mères de bébés avec un attachement désorganisé et ambivalent que chez les mères de bébés étant attachés de manière sécure. Les résultats soutiennent le lien entre la communication perturbée et l'attachement désorganisé dans la société arabe en Israël et ils s'ajoutent également à notre compréhension du comportement maternel lié à l'attachement ambivalent.