Canopy and understory nitrogen additions differently affect soil microbial residual carbon in a temperate forest.

Atmospheric nitrogen (N) deposition in forests can affect soil microbial growth and turnover directly through increasing N availability and indirectly through altering plant-derived carbon (C) availability for microbes. This impacts microbial residues (i.e., amino sugars), a major component of soil organic carbon (SOC). Previous studies in forests have so far focused on the impact of understory N addition on microbes and microbial residues, but the effect of N deposition through plant canopy, the major pathway of N deposition in nature, has not been explicitly explored. In this study, we investigated whether and how the quantities (25 and 50 kg N ha-1 year-1) and modes (canopy and understory) of N addition affect soil microbial residues in a temperate broadleaf forest under 10-year N additions. Our results showed that N addition enhanced the concentrations of soil amino sugars and microbial residual C (MRC) but not their relative contributions to SOC, and this effect on amino sugars and MRC was closely related to the quantities and modes of N addition. In the topsoil, high-N addition significantly increased the concentrations of amino sugars and MRC, regardless of the N addition mode. In the subsoil, only canopy N addition positively affected amino sugars and MRC, implying that the indirect pathway via plants plays a more important role. Neither canopy nor understory N addition significantly affected soil microbial biomass (as represented by phospholipid fatty acids), community composition and activity, suggesting that enhanced microbial residues under N deposition likely stem from increased microbial turnover. These findings indicate that understory N addition may underestimate the impact of N deposition on microbial residues and SOC, highlighting that the processes of canopy N uptake and plant-derived C availability to microbes should be taken into consideration when predicting the impact of N deposition on the C sequestration in temperate forests.

Climatic seasonality challenges the stability of microbial-driven deep soil carbon accumulation across China.

Microbial residues contribute to the long-term stabilization of carbon in the entire soil profile, helping to regulate the climate of the planet; however, how sensitive these residues are to climatic seasonality remains virtually unknown, especially for deep soils across environmental gradients. Here, we investigated the changes of microbial residues along soil profiles (0-100 cm) from 44 typical ecosystems with a wide range of climates (~3100 km transects across China). Our results showed that microbial residues account for a larger portion of soil carbon in deeper (60-100 cm) vs. shallower (0-30 and 30-60 cm) soils. Moreover, we find that climate especially challenges the accumulation of microbial residues in deep soils, while soil properties and climate share their roles in controlling the residue accumulation in surface soils. Climatic seasonality, including positive correlations with summer precipitation and maximum monthly precipitation, as well as negative correlations with temperature annual range, are important factors explaining microbial residue accumulation in deep soils across China. In particular, summer precipitation is the key regulator of microbial-driven carbon stability in deep soils, which has 37.2% of relative independent effects on deep-soil microbial residue accumulation. Our work provides novel insights into the importance of climatic seasonality in driving the stabilization of microbial residues in deep soils, challenging the idea that deep soils as long-term carbon reservoirs can buffer climate change.

Microbial necromass under global change and implications for soil organic matter.

Microbial necromass is an important source and component of soil organic matter (SOM), especially within the most stable pools. Global change factors such as anthropogenic nitrogen (N), phosphorus (P), and potassium (K) inputs, climate warming, elevated atmospheric carbon dioxide (eCO2 ), and periodic precipitation reduction (drought) strongly affect soil microorganisms and consequently, influence microbial necromass formation. The impacts of these global change factors on microbial necromass are poorly understood despite their critical role in the cycling and sequestration of soil carbon (C) and nutrients. Here, we conducted a meta-analysis to reveal general patterns of the effects of nutrient addition, warming, eCO2 , and drought on amino sugars (biomarkers of microbial necromass) in soils under croplands, forests, and grasslands. Nitrogen addition combined with P and K increased the content of fungal (+21%), bacterial (+22%), and total amino sugars (+9%), consequently leading to increased SOM formation. Nitrogen addition alone increased solely bacterial necromass (+10%) because the decrease of N limitation stimulated bacterial more than fungal growth. Warming increased bacterial necromass, because bacteria have competitive advantages at high temperatures compared to fungi. Other global change factors (P and NP addition, eCO2 , and drought) had minor effects on microbial necromass because of: (i) compensation of the impacts by opposite processes, and (ii) the short duration of experiments compared to the slow microbial necromass turnover. Future studies should focus on: (i) the stronger response of bacterial necromass to N addition and warming compared to that of fungi, and (ii) the increased microbial necromass contribution to SOM accumulation and stability under NPK fertilization, and thereby for negative feedback to climate warming.

Microbial pathways driving stable soil organic carbon change in abandoned Moso bamboo forests in southeast China.

Mineral-associated organic carbon (MOC) is a stable component of the soil carbon (C) pool, critical to realize carbon sequestration and coping with climate change. Many Moso bamboo (Phyllostachys edulis) forests in subtropical and tropical areas that used to be intensively managed have been left unmanaged. Still, studies on MOC changes occurring during the transition from intensive management to unmanagement are lacking. Besides, the understanding of the role of microorganisms in MOC accumulation is far from satisfactory. Based on the combination of field investigation and laboratory analysis of 40 Moso bamboo forest sampling plots with different unmanaged chronosequence's in southeast China, we observed the MOC content in Moso bamboo forests left unmanaged for 2-5 years had decreased, whereas that in forests left unmanaged for 11-14 years had increased compared with that in intensively managed forests. Specifically, the MOC contents in forests left unmanaged for 11-14 years were significantly higher than in those under intensive management or unmanaged for 2-5 years. Moreover, we found that microorganisms drove MOC change through two different pathways: (i) more microorganisms led to more soil nutrients, which led to more amino sugars, ultimately resulting in the accumulation of MOC, and (ii) microorganisms promoted the accumulation of MOC by influencing the content of metal oxides (poorly crystalline aluminum oxides and free aluminum oxides). We believe that ignoring the interaction between microorganisms and metal oxides may lead to uncertainty in evaluating the relative contribution of microbial residues to MOC.

Melanin mitigates the accelerated decay of mycorrhizal necromass with peatland warming.

Despite being a significant input into soil carbon pools of many high-latitude ecosystems, little is known about the effects of climate change on the turnover of mycorrhizal fungal necromass. Here, we present results from the first experiment examining the effects of climate change on the long-term decomposition of mycorrhizal necromass, utilising the Spruce and Peatland Response Under Changing Environments (SPRUCE) experiment. Warming significantly increased necromass decomposition rates but was strongest in normally submerged microsites where warming caused water table drawdown. Necromass chemistry exerted the strongest control on the decomposition, with initial nitrogen content strongly predicting early decay rates (3 months) and initial melanin content determining mass remaining after 2 years. Collectively, our results suggest that as global temperatures rise, variation in species biochemical traits as well as microsites where mycorrhizal necromass is deposited will determine how these important inputs contribute to the belowground storage of carbon in boreal peatlands.

Divergent assembly of soil microbial necromass from microbial and organic fertilizers in Chimonobambusa hejiangensis forest.

Introduction: Variability in microbial residues within soil aggregates are becoming progressively essential to the nutritive and sustainability of soils, and are therefore broadly regarded as an indispensable part of soil organic matter. It is unexplored how the widespread implementation of microbial fertilisers in agricultural production impacts soil organic nutrients, in particular the microbial residue fraction. Methods: We performed a three-year field experiment to verify the distinct impacts of microbial and organic fertilizers on carbon accumulation in soil microbial leftovers among aggregate fractions. Results: Microbial residual carbon was shown to decrease insignificantly during the application of microbial fertilizer and to rise marginally afterwards with the utilization of organic fertilizer. However, the combined effects of the two fertilizers had substantial impacts on the accumulation of microbial residual carbon. Changes in the structure of the fungi and bacteria shown in this study have implications for the short-term potential of microbial fertilizer shortages to permanent soil carbon sequestration. Additionally, our findings revealed variations in microbial residue accumulation across the microbial fertilizers, with Azotobacter chroococcum fertilizer being preferable to Bacillus mucilaginosus fertilizer due to its higher efficiency. In this scenario of nutrient addition, fungal residues may serve as the primary binding component or focal point for the production of new microaggregates, since the quantity of SOC provided by fungal residues increased while that supplied by bacterial residues decreased. Discussion: Our findings collectively suggested that the mechanisms behind the observed bacterial and fungal MRC (microbial residue carbon) responses to microbial fertilizer or organic fertilizer in bamboo forest soils are likely to be distinct. The application of microbial fertilizers for a limited duration led to a decline soil stable carbon pool, potentially influencing the regulation of soil nutrients in such hilly bamboo forests.

Long-term plastic mulching decreases rhizoplane soil carbon sequestration by decreasing microbial anabolism.

Ridge-furrow with plastic mulching (RFPM) is a widely used agricultural practice in rain-fed farmlands. However, the impact of microbial related metabolism on soil organic carbon (SOC) is not fully understood. Amino sugar analysis, high-throughput sequencing, and high-throughput qPCR approaches are combined to investigate this topic, based on a long-term experiment. Treatments include flat planting without mulching (FP), ridge-furrow without mulching (RF), and RFPM. RFPM significantly decreases rhizoplane SOC contents, while bulk SOC contents change insignificantly across treatments. In terms of microbial metabolic pathways, RFPM decreases indicators of the in vivo metabolic pathway, whereas those of the ex vivo pathway are increased. In terms of microbial community features, core taxa module #1 is dominated by Sphingomonadaceae. These are putative high yield (Y) strategists, according to the microbial life-history strategy framework. They are closely related to the in vivo pathway and are most predictive for SOC; their abundance is highest under FP and lowest under RFPM. Core taxa module #2 is dominated by Chitinophagaceae, putative resource acquisition (A) strategists, that are closely related to the ex vivo pathway. Their abundance in the rhizoplane is highest under RFPM and lowest under FP. The RFPM-induced decline in SOC occurs simultaneously with the abundance of A-strategists with in vivo pathway but not the Y-strategists with ex vivo pathway. Overall, the result of this study shows a trade-off. In RFPM practice, the ex vivo microbial pathway is enhanced along with the abundance of A-strategists. This is not the case for the in vivo pathway and associated abundance of Y-strategists, which are closely associated with SOC. Our findings underlined the impact of rhizoplane microbial metabolic pathways on SOC status is key to agricultural practices in drylands such as RFPM, and advanced our understanding of how microbes affect the carbon cycling in dryland farming.

Loss of soil microbial residue carbon by converting a tropical forest to tea plantation.

Land-use change can lead to profound changes in the storage of soil organic carbon (SOC) in the tropics. Soil microbial residues make up the majority of persistent SOC pools, yet the impact of land-use change on microbial residue C accumulation in the tropics is not well understood. Here, we investigated how the conversion of tropical primary montane rainforest to secondary forest and the conversions of secondary forest to Prunus salicina plantation and tea plantation, influence the accumulation of soil microbial residue C (indicated by amino sugars). Our results showed that the secondary forest had a higher SOC than that of the primary forest (+63%), while they had no difference in microbial residue C concentration, indicating a relatively slow microbial-derived C accrual during secondary succession. Moreover, the P. salicina plantation and tea plantation had lower SOC than the secondary forest (-53% and -57%, respectively). A decrease in fungal biomass (-51%) resulted in less fungal and total residue C concentrations in the tea plantation than in the secondary forest (-38% and -35%, respectively), indicating microbial-derived C loss following the forest conversion. The change in microbial residue C depended on litter standing crop rather than soil nutrient and root biomass. Litter standing crop affected microbial residue C concentration by regulating fungal biomass and hydrolytic enzyme activities. Taken together, our results highlight that litter-microbe interactions drive microbial residue C accumulation following forest conversions in the tropics.

Dynamics of microbial residues control the responses of mineral-associated soil organic carbon to N addition in two temperate forests.

Numerous studies have investigated the impact of nitrogen (N) addition on ecosystem carbon (C) storage and cycling. However, how N addition regulates the dynamics of different soil organic carbon (SOC) fractions and the underlying microbial mechanisms remain unclear. In this study, we assessed microbial controls (through biomass, residues and enzymes) of different SOC fractions (particulate organic carbon, POC and mineral-associated organic carbon, MAOC) in response to six years of N addition (50 kg N ha-1 yr-1) in two temperate forests (Betula platyphylla vs. Quercus wutaishanica) in Northern China. Plant inputs (root biomass and leaf litterfall) and soil chemistry (pH, extractable inorganic N, and exchangeable cations) were unaltered by N addition in both forests. In the Q. wutaishanica forest, microbial biomass, residues, and enzymes were not sensitive to N addition, which may explain the lack of response in SOC and two fractions (POC and MAOC). However, in the B. platyphylla forest, although microbial biomass and enzymes as well as SOC and POC did not significantly change after N addition, both microbial residues (amino sugars) and MAOC significantly increased after N addition. Moreover, there was a strong positive correlation between microbial residues and MAOC pool within or across the two forests. Collectively, these results suggest that the dynamics of microbial residues play a crucial role in controlling the response of mineral-associated SOC to N addition in these two forests. Separating bulk soil into distinct functional pools and considering microbial residues should help reveal the nuanced response of soil C dynamics under N addition.

Responses of microbial residues to simulated climate change in a semiarid grassland.

Microbial residues play important role in regulating soil carbon (C) turnover and stability, but the responses of microbial residues to climate change are neglected. In this study, a 5-year field experiment that simulated two climate change factors (precipitation and warming) was performed to examine microbial residue changes in a semiarid grassland, with water limitation. Both the contents of total amino sugars (a biomarker of microbial residues) and glucosamine (a biomarker of fungal residues) increased significantly with increased precipitation and decreased under warming, whereas neither increased precipitation nor warming influenced the content of muramic acid (a biomarker of bacterial residues). These findings clarified the role of fungal residues in determining the response of microbial residues to altered water availability and plant productivity induced by increased precipitation and elevated temperature. Interestingly, microbial residues had a much greater response to climate change than total soil C, implying that soil C composition and stability altered prior to soil C storage and simultaneously slowed down the change of soil C pool. Integrating microbial residues into current climate-C models is expected to enable the models to more accurately evaluate soil C responses to climate regimes in semiarid grasslands.