Nitrogen stock and farmer behaviour under rice policy change in Japan.

Mitigating the environmental impact of nitrogen (N) derived from agricultural production is a significant issue in our progression towards a sustainable society. This study aims to estimate the N stock (i.e., as the accumulation of N input into farmland that is neither taken up by the crops nor discharged into the environment, but rather remains in the soil) related to rice production in Japan and to identify the relationship between the economic behaviour of rice farmers and N stock. The results indicate a persistent reduction in N stock since the early 1990s, caused mainly by the change in N input by a change in government policy away from production subsidies to more extensive use of market mechanisms. It seems clear that production subsidy policies provide little incentive for farmers to reduce their N input; however, even if the introduction of market mechanisms, farmers may not properly evaluate the contribution of N stock to agricultural production. Consequently, it may be difficult to achieve the optimal level of N stock from an environmental impact perspective. To achieve the optimal N stock, the relationship between farmer behaviour and N stock needs to be better understood.

Research progress on isotope tracing on the sources and transformations of reactive nitrogen in the earth-atmosphere system.

Since the Industrial Revolution, human activities have led to a rapid and sustained increase in reactive nitrogen production, resulting in nitrogen enrichment at the Earth's surface and triggering many ecological and environmental issues. Stable isotopes are effective tools for tracing the sources and mechanisms of environmental processes. The nitrogen isotope values in surface environments integrate the isotope signatures of different nitrogen sources and the isotope fractionation effects of transformation processes. The composition of nitrogen isotopes can thus be utilized to trace the sources and cycling of nitrogen at the surface, aiding the development of strategies to reduce reactive nitrogen emissions, and assess the ecological effects of nitrogen enrichment. We reviewed the research progress on nitrogen isotope in the sources of reactive nitrogen in atmospheric systems, plant nitrogen utilization, and tracking of nitrogen processes in forest ecosystems. We further discussed how to gain a more systematic and accurate understanding of nitrogen cycle within and between the various spheres of the surface environment.

[Characteristics of nitrogen wet deposition and flux with runoff during the spring freeze-thaw period in permafrost region watershed of Da Hinggan Mountains, Northeast China]. / å¤§å ´å®‰å²­å¤šå¹´å†»åœŸåŒºæµåŸŸæ˜¥å­£å†»èžæœŸæ°®æ¹¿æ²‰é™ä¸Žæ°´ä½“æ°®è¾“å‡ºç‰¹å¾.

Nitrogen (N) transport is an important component of a watershed's nutrient cycle, which has significant impacts on global nitrogen cycle. In this study, we measured precipitation and daily stream N concentrations during the spring freeze-thaw period (April 9-June 30, 2021) in a small forest watershed (Laoyeling) in the permafrost region of Da Hinggan Mountains to calculate wet N deposition and stream N flux. The results showed the wet deposition fluxes of ammonium, nitrate, and total N were 695.88, 448.72 and 1947.35 g·hm-2, respectively, while the stream N fluxes were 86.37, 186.87 and 1160.78 g·hm-2, respectively over the whole study period. Precipitation was the main influencing factor of wet N deposition. During the freeze-thaw cycle period (April 9 to 28), the stream N flux was dominated by runoff and was affected by soil temperature through its influence on runoff. During the melting period (April 29 to June 30), it was affected by both runoff and runoff N concentration. The stream total N flux accounted for 59.6% of wet deposition during the study period, which indicated that the watershed had a strong N fixation potential. These findings would have important implications for understanding the impact of climate change on N cycling in permafrost watersheds.

Dietary Inclusion of Crickets (Acheta domesticus) and Yellow Mealworm Meal (Tenebrio molitor) in Comparison with Soybean Meal: Effect on the Growth, Total Tract Apparent Digestibility, and Nitrogen Balance of Fattening Rabbits.

Three diets were formulated, i.e., soybean meal (SM diet), adult Acheta domesticus (AD diet), and Tenebrio molitor larvae (TM diet), as the main crude protein (CP) sources. A total of 45 rabbits (Hyplus, weaned at 32 days of age) were divided into three groups (15 per treatment) and fed one of the three diets for 42 days. A higher daily weight gain (p = 0.042), as well as daily feed intake (p = 0.022), was observed in rabbits fed the AD and TM diets than in rabbits fed the SM diet within 21 days after weaning. The coefficients of total tract apparent digestibility (CTTAD) of gross energy were higher (p = 0.001) in rabbits fed the SM diet than in rabbits fed the other diets. The CTTAD of CP (p = 0.040) and starch (p = 0.041) was higher in rabbits fed the SM diet compared to those fed the AD diet. There were non-significantly higher losses of nitrogen in the urine (an average by 0.227 g/day; p = 0.094) in rabbits fed the TM diet than in rabbits fed the other diets. It can be concluded that the growth of rabbits and nitrogen output were not detrimentally affected by the insect meal (AD or TM) used in this study.

Nutrient digestibility, fecal output of fractionated proteins, and ruminal fermentation parameters of goats fed a diet supplemented with spent green tea and black tea leaf silage.

Spent green and black tea leaf silage (GTS and BTS, respectively) was offered as a protein supplement to goats to examine in vivo digestibility, nitrogen balance, urinary excretion of purine derivatives, and ruminal fermentation. Four castrated goats were fed a basal diet supplemented with alfalfa hay cube (AHC), GTS, or BTS in a 4 × 4 Latin square design. Digestibilities of various nutrients except for nitrogen (N) fraction were unaffected by the type of supplement. Digestibility of acid detergent insoluble N (ADIN) in BTS treatment was a negative value and significantly lower than those in other treatments. Urinary N output and retained N were not significantly affected by the diets. The fecal output of neutral detergent insoluble nitrogen (NDIN) and ADIN in the BTS treatment was significantly higher than those in other treatments. Urinary excretion of purine derivatives was not affected by the treatments. Ruminal NH3 -N concentration in AHC and GTS treatments were not significantly different, but that in the BTS treatment was significantly lower than others. These results indicated that GTS is substitutable for AHC as a protein supplement, whereas BTS was able to bind proteins tightly in the digestive tract, which lowered ruminal N degradability and increase fecal N output.

Measuring the zonal responses of nitrogen output to landscape pattern in a flatland with river network: a case study in Taihu Lake Basin, China.

Landscape pattern changes induced by rapid urbanization and intensified agricultural activities have exerted great pressure on regional water purification services. Relationship between landscape metrics and nitrogen-related ecosystem services has been a major concern of many scholars and has been widely used for guidance for land use and cover (LULC) management. However, clear zonal differences may exist, especially in highly developed reticular river network area, thus limiting our understanding of nitrogen output (NOP) to landscape pattern in the details. The spatial distribution of regional NOP was obtained based on the InVEST model. The zonal responses of NOP to landscape patter were examined under hydraulic subregions and subbasin scale. The results show that the unit value of average NOP in the Taihu Lake Basin (TLB) was 146.14 (kg/km2), and the total output reached 23677.92 t in 2020. The simulation NOP showed reasonable agreement with verified water quality observations in the lake inlet stations, with an R2 of 0.76. In terms of space composition, merely cropland have significant effects on NOP in the whole basin scale, while the explanatory variables include cropland and developed land in Pudong (PD), Puxi (PX), Wuchengxiyu (WC), and Hangjiahu (HJ) regions. In Huxi (HX) and Yangchengdianmao  (YC) regions, cropland and forest are the significant impact types, while in (Zhexi) ZX region, cropland, developed land, and forest are significant impact types. In the space configuration, the percentage of landscape (PLAND) or largest patch index (LPI) of cropland showed positive effects about NOP, whether in the whole basin or the hydraulic subregions. Edge density (ED) (-3.48), number of patches (NP) (-3.91), and percentage of like adjacencies (PLAND) (-2.80) of the forest exhibit negative correlations with NOP, in the HX, ZX, and YC region, respectively. It displays diversiform in the response of NOP to the landscape metric of developed land, which speculate that the heterogeneity of developed land can also have a constraint on NOP, in the highly urbanized areas with less forest area. In addition, the total nitrogen output of the TLB needs to be controlled, especially in HJ region which was identified as the sensitive area of pollution sources with the largest NOP and should be paid more attention to. Compared with the administrative management unit, it is more reasonable to control and manage the pollution sources by referring to the hydraulic subregions and subbasin units. Senior managers are required to strengthen communication and cooperation with hydraulic subregions across administrative regions. However, when managing NOP through the landscape modifications, measures should be taken to reduce the aggregation of nitrogen sources and increase the fragmentation of nitrogen sinks. As for high aggregation developed and agricultural land regions, the types of land used should be enriched to help the sustainable development.

Estimation of the maintenance energy requirements, methane emissions and nitrogen utilization efficiency of two suckler cow genotypes.

Seventeen non-lactating dairy-bred suckler cows (LF; Limousin×Holstein-Friesian) and 17 non-lactating beef composite breed suckler cows (ST; Stabiliser) were used to study enteric methane emissions and energy and nitrogen (N) utilization from grass silage diets. Cows were housed in cubicle accommodation for 17 days, and then moved to individual tie-stalls for an 8-day digestibility balance including a 2-day adaption followed by immediate transfer to an indirect, open-circuit, respiration calorimeters for 3 days with gaseous exchange recorded over the last two of these days. Grass silage was offered ad libitum once daily at 0900 h throughout the study. There were no significant differences (P>0.05) between the genotypes for energy intakes, energy outputs or energy use efficiency, or for methane emission rates (methane emissions per unit of dry matter intake or energy intake), or for N metabolism characteristics (N intake or N output in faeces or urine). Accordingly, the data for both cow genotypes were pooled and used to develop relationships between inputs and outputs. Regression of energy retention against ME intake (r 2=0.52; P<0.001) indicated values for net energy requirements for maintenance of 0.386, 0.392 and 0.375 MJ/kg0.75 for LF+ST, LF and ST respectively. Methane energy output was 0.066 of gross energy intake when the intercept was omitted from the linear equation (r 2=0.59; P<0.001). There were positive linear relationships between N intake and N outputs in manure, and manure N accounted for 0.923 of the N intake. The present results provide approaches to predict maintenance energy requirement, methane emission and manure N output for suckler cows and further information is required to evaluate their application in a wide range of suckler production systems.

[Land Use Structure Change and Its Control Effect of Nitrogen Output in a Small Watershed of Three Gorges Reservoir Area: A Case Study of Lanlingxi Watershed].

The nitrogen (N) output in Lanlingxi watershed of Three Gorges Reservoir Area in 2015 was monitored and the current land use map was investigated. Cluster analysis and correlation analysis were used to identify the major sources of pollutants and to discriminate the source and the sink land use types. The effects of land use on N exports were quantitatively analyzed by stepwise regression analysis. The results showed that: ① After the returning farmland to forest project the land use structure of this area changed dramatically. The area proportions of forest land and garden plot increased to 76.85% and 13.87% respectively, and the proportion of cultivated land dropped to 1.16%. Cultivated lands were stellate distributed and garden plots in some catchments were flake distributed. ② The content of TN in some monitoring points surpassed the Class V standard of the national groundwater environmental quality. The ammonium-N (NH4+-N) concentrations ranged 0.089-0.214 mg·L-1, 2.925-13.203 mg·L-1 for nitrate-N (NO3--N) and 3.561-14.572 mg·L-1 for total-N (TN). And NO3--N accounted for more than 80% of TN. ③ There were significant positive correlations between garden plot, residential land and N loss, and negative correlations between forest land, unutilized land and N export, which indicated that the former were N sources and the latter were sinks. ④ Forest land area should be increased and garden plot should be controlled, and the area ratio of residential land should be kept below 5% when adjusting land use structure of this watershed. Moreover, forest-tea and forest-fruits modes should be applied to change single land use type of garden plot in some catchments.