Emerging trends to replace pesticides with nanomaterials: Recent experiences and future perspectives for ecofriendly environment.

Despite the widespread usage to safeguard crops and manage pests, pesticides have detrimental effects on the environment and human health. The necessity to find sustainable agricultural techniques and meet the growing demand for food production has spurred the quest for pesticide substitutes other than traditional ones. The unique qualities of nanotechnology, including its high surface area-to-volume ratio, controlled release, and better stability, have made it a promising choice for pest management. Over the past ten years, there has been a noticeable growth in the usage of nanomaterials for pest management; however, concerns about their possible effects on the environment and human health have also surfaced. The purpose of this review paper is to give a broad overview of the worldwide trends and environmental effects of using nanomaterials in place of pesticides. The various types of nanomaterials, their characteristics, and their possible application in crop protection are covered. The limits of the current regulatory frameworks for nanomaterials in agriculture are further highlighted in this review. Additionally, it describes how standard testing procedures must be followed to assess the effects of nanomaterials on the environment and human health before their commercialization. In order to establish sustainable and secure nanotechnology-based pest control techniques, the review concludes by highlighting the significance of taking into account the possible hazards and benefits of nanomaterials for pest management and the necessity of an integrated approach. It also emphasizes the importance of more investigation into the behavior and environmental fate of nanomaterials to guarantee their safe and efficient application in agriculture.

Pesticide-tolerant microbial consortia: Potential candidates for remediation/clean-up of pesticide-contaminated agricultural soil.

Reclamation of pesticide-polluted lands has long been a difficult endeavour. The use of synthetic pesticides could not be restricted due to rising agricultural demand. Pesticide toxicity has become a pressing agronomic problem due to its adverse impact on agroecosystems, agricultural output, and consequently food security and safety. Among different techniques used for the reclamation of pesticide-polluted sites, microbial bioremediation is an eco-friendly approach, which focuses on the application of resilient plant growth promoting rhizobacteria (PGPR) that may transform or degrade chemical pesticides to innocuous forms. Such pesticide-resilient PGPR has demonstrated favourable effects on soil-plant systems, even in pesticide-contaminated environments, by degrading pesticides, providing macro-and micronutrients, and secreting active but variable secondary metabolites like-phytohormones, siderophores, ACC deaminase, etc. This review critically aims to advance mechanistic understanding related to the reduction of phytotoxicity of pesticides via the use of microbe-mediated remediation techniques leading to crop optimization in pesticide-stressed soils. The literature surveyed and data presented herein are extremely useful, offering agronomists-and crop protectionists microbes-assisted remedial strategies for affordably enhancing crop productivity in pesticide-stressed soils.

Transcriptome Profiling of Etridiazole-Exposed Zebrafish (Danio rerio) Embryos Reveals Pathways Associated with Cardiac and Ocular Toxicities.

Etridiazole (EDZ) is a thiadiazole-containing fungicide commonly used to control Pythium and Phytophthora spp. Although previous studies have shown that EDZ is teratogenic, the exact molecular mechanisms underlying its toxicity remain unknown. In this study, a zebrafish (Danio rerio; ZF) model was used to explore the molecular pathways associated with EDZ toxicity. The whole transcriptome of ZF embryos exposed to 96 h of EDZ was analyzed, along with developmental abnormalities. EDZ-induced malformations were primarily related to the eyes, heart, and growth of the ZF. Compared to untreated ZF, etridiazole-treated ZF had 2882 differentially expressed genes (DEGs), consisting of 1651 downregulated genes and 1231 upregulated genes. Gene ontology enrichment analysis showed that DEGs were involved in biological processes, such as sensory perception, visual perception, sensory organ development, and visual system development, and showed transmembrane transporter and peptidase regulator activities. Metabolism, phototransduction, aminoacyl-tRNA biosynthesis, MAPK signaling pathway, calcium signaling pathway, and vascular smooth muscle contraction were among the most enriched KEGG pathways. The qPCR analyses of the eight random genes were in good agreement with the transcriptome data. These results suggest several putative mechanisms underlying EDZ-induced developmental deformities in ZF.

The system of self-consistent models for pesticide toxicity to Daphnia magna.

Quantitative structure-property/activity relationships (QSPRs/QSARs) are a tool of modern theoretical and computational chemistry. The self-consistent model system is both a method to build up a group of QSPR/QSAR models and an approach to checking the reliability of these models. Here, a group of models of pesticide toxicity toward Daphnia magna for different distributions into training and test sub-sets is compared. This comparison is the basis for formulating the system of self-consistent models. The so-called index of the ideality of correlation (IIC) has been used to improve the above models' predictive potential of pesticide toxicity. The predictive potential of the suggested models should be classified as high since the average value of the determination coefficient for the validation sets is 0.841, and the dispersion is 0.033 (on all five models). The best model (number 4) has an average determination coefficient of 0.89 for the external validation sets (related to all five splits).

Temephos, an organophosphate larvicide for residential use: a review of its toxicity.

Temephos (O,O,O',O'-tetramethyl O,O'-thiodi-p-phenylene bis(phosphorothioate)) is a larvicide belonging to the family of organophosphate pesticides used for the control of different vectors of diseases, such as dengue, Zika, chikungunya, and dracunculiasis. The aim of this review was to discuss the available published information about temephos toxicokinetics and toxicity in mammals. Temephos is quickly absorbed in the gastrointestinal tract, distributed to all organs, and then it accumulates mainly in adipose tissue. It is metabolized by S-oxidation, oxidative desulfuration, and hydrolysis reactions, with the possible participation of cytochrome P450 (CYP). Temephos is mainly eliminated by feces, whereas some of its metabolites are eliminated by urine. The World Health Organization classifies it as class III: slightly dangerous with a NOAEL (no-observed adverse effect level) of 2.3 mg/kg/day for up to 90 days in rats, based on brain acetylcholinesterase (AChE) inhibition. A LOAEL (lowest observable adverse effect level) of 100 mg/kg/day for up to 44 days in rats was proposed based on cholinergic symptoms. However, some studies have shown that temephos causes toxic effects in mammals. The inhibition of the enzyme acetylcholinesterase (AChE) is one of its main demonstrated effects; however, this larvicide has also shown genotoxic effects and some adverse effects on male reproduction and fertility, as well as liver damage, even at low doses. We performed an extensive review through several databases of the literature about temephos toxicokinetics, and we recommend to revisit current assessment of temephos with the new available data.

Carbofuran self-poisoning: forensic and analytic investigations in twins and literature review.

Carbofuran is a pesticide widely used in agricultural context to kill insects, mites, and flies by ingestion or contact. Along with literature review, we aimed to (i) present the clinical, autopsy, and toxicological findings of carbofuran self-poisonings in two 69-year-old twins, resulting in the death of one of them and (ii) assess carbofuran metabolite distribution using molecular networking. Quantitative analysis of carbofuran and its main metabolites (3-hydroxycarbofuran and 3-ketocarbofuran) was carried out using an original liquid chromatography-tandem mass spectrometry method on biological samples (cardiac or peripheral blood, urine, bile, and gastric contents). Toxicological analysis of post-mortem samples (twin 1) highlighted high concentrations of carbofuran and its metabolites in cardiac blood, bile, and gastric contents. These compounds were also quantified in blood and/or urine samples of the living brother (twin 2), confirming poisoning. Using molecular networking approach to facilitate visualization of mass spectrometry datasets and sample-to-sample comparisons, we detected two more metabolites (7-phenol-carbofuran and 3-hydroxycarbofuran glucuronide) in bile (twin 1) and urine (twin 2). These results highlight the value of (i) these compounds as carbofuran consumption markers and (ii) bile samples in post-mortem analysis to confirm poisoning. From an analytical point of view, molecular networking allowed the detection and interpretation of carbofuran metabolite ammonium adducts which helped to confirm their identification annotations, as well as their structural data. From a clinical point of view, the different outcomes between the two brothers are discussed. Overall, these cases provide novel information regarding the distribution of carbofuran and its metabolites in poisoning context.

The agrochemical S-metolachlor disrupts molecular mediators and morphology of the swim bladder: Implications for locomotor activity in zebrafish (Danio rerio).

Metolachlor herbicides are derived from the chloroacetamide chemical family of which there are the S- and R-metolachlor isomers. S-metolachlor is a selective herbicide that inhibits cell division and mitosis via enzyme interference. The herbicide is used globally in agriculture and studies report adverse effects in aquatic organisms; however, there are no studies investigating sub-lethal effects of S-metolachlor on swim bladder formation, mitochondrial ATP production, nor light-dark preference behaviors in fish. These endpoints are relevant for larval locomotor activity and metabolism. To address these knowledge gaps, we exposed zebrafish embryos/larvae to various concentrations of S-metolachlor (0.5-50 µM) over early development. S-metolachlor affected survival, hatching percentage, and increased developmental deformities at concentrations of 50 µM and above. Exposure levels as high as 200 µM for 24 and 48 h did not alter oxygen consumption rates in zebrafish, and there were no changes detected in endpoints related to mitochondrial oxidative phosphorylation. We observed impairment of swim bladder inflation at 50 µM in 6 dpf larvae. To elucidate mechanisms related to this, we measured relative transcript abundance for genes associated with the swim bladder (smooth muscle alpha (α)-2 actin, annexin A5, pre-B-cell leukemia homeobox 1a). Smooth muscle alpha (α)-2 actin mRNA levels were reduced in fish exposed to 50 µM while annexin A5 mRNA levels were increased in abundance, corresponding to reduced swim bladder size in larvae. A visual motor response test revealed that larval zebrafish exhibited some hyperactivity in the light with exposure to the herbicide and only the highest dose tested (50 µM) resulted in hypoactivity in the dark cycle. Regression analysis indicated that there was a positive relationship between surface area of the swim bladder and distance traveled, and the size of the swim bladder explained ~10-14% in the variation for total distance moved. Lastly, we tested larvae in a light dark preference test, and we did not detect any altered behavioral response to any concentration tested. Here we present new data on sublethal endpoints associated with exposure to the herbicide S-metolachlor and demonstrate that this chemical may disrupt transcripts associated with swim bladder formation and morphology, which could ultimately affect larval zebrafish activity. These data are expected to contribute to further risk assessment guidelines for S-metolachlor in aquatic ecosystems.

Pesticide Mixtures in the Water-Column Versus Bottom-Sediments of Prairie Rivers.

River water-column and bottom-sediments samples were screened for 160 pesticide compounds to compare the types of pesticides present in the water-column versus bottom-sediments, and between segments of rivers flowing through intensively-managed versus semi-natural habitats. Of the 35 pesticide compounds detected, current-use pesticides accounted for 96% (water) and 76% (bottom sediments). Pesticide mixtures were present in 72% (water) and 51% (sediment) of the total samples. Only the river flowing through the most intensively managed habitat showed a wide range of pesticides in sediments, and many of these pesticides were also present in the water-column of that river. Current-use fungicides were detected in both the water-column and bottom-sediments but not in samples taken from rivers flowing predominantly through semi-natural habitats. The study period (May to August) corresponds to the peak time of regional pesticide applications and hence the time period that is most likely to show elevated concentrations of current-use pesticides in the water-column. The environmental concentrations of pesticide mixtures detected in the water-column were used to calculate Pesticide Toxicity Index (PTI) values as it applies to non-vascular or vascular plants, invertebrates, and fish. The PTI values were largest for non-vascular and vascular plants, reflecting that the pesticide mixtures in water-column were dominated by herbicides.

Mining the effect of the neonicotinoids imidacloprid and clothianidin on the chemical homeostasis and energy equilibrium of primary mouse neural stem/progenitor cells using metabolomics.

The projection of plant protection products' (PPPs) toxicity to non-target organisms at early stages of their development is challenging and demanding. Recent developments in bioanalytics, however, have facilitated the study of fluctuations in the metabolism of biological systems in response to treatments with bioactives and the discovery of corresponding toxicity biomarkers. Neonicotinoids are improved insecticides that target nicotinic acetylocholine receptors (nAChR) in insects which are similar to mammals. Nonetheless, they have sparked controversy due to effects on non-target organisms. Within this context, mammalian cell cultures represent ideal systems for the development of robust models for the dissection of PPPs' toxicity. Thus, we have investigated the toxicity of imidacloprid, clothianidin, and their mixture on primary mouse (Mus musculus) neural stem/progenitor (NSPCs) and mouse neuroblastoma-derived Neuro-2a (N2a) cells, and the undergoing metabolic changes applying metabolomics. Results revealed that NSPCs, which in vitro resemble those that reside in the postnatal and adult central nervous system, are five to seven-fold more sensitive than N2a to the applied insecticides. The energy equilibrium of NSPCs was substantially altered, as it is indicated by fluctuations of metabolites involved in energy production (e.g. glucose, lactate), Krebs cycle intermediates, and fatty acids, which are important components of cell membranes. Such evidence plausibly suggests a switch of cells' energy-producing mechanism to the direct metabolism of glucose to lactate in response to insecticides. The developed pipeline could be further exploited in the discovery of unintended effects of PPPs at early steps of development and for regulatory purposes.

In vitro investigation to explore the toxicity of different groups of pesticides for an agronomically important rhizosphere isolate Azotobacter vinelandii.

In this work, an attempt was made to evaluate the effect of pesticides on growth pattern, surface morphology, cell viability and growth regulators of nitrogen fixing soil bacterium. Pesticide tolerant Azotobacter vinelandii strain AZ6 (Accession no. MG028654) was found to tolerate maximum level of pesticide and displayed multifarious PGP activities. At higher concentrations, pesticides triggered cellular/structural damage and reduced the cell viability as clearly shown under SEM and CLSM. With increase in concentration, pesticides exhibited a significant (p < 0.05) decrease in PGP traits of strain AZ6. Among all three groups of pesticides, herbicides glyphosate and atrazine were most toxic. Kitazin, hexaconazole, metalaxyl, glyphosate, quizalofop, atrazine, fipronil, monocrotophos and imidacloprid at 2400, 1800, 1500, 900, 1200, 900, 1800, 2100 and 2700 µg mL-1, respectively, decreased the production of IAA by 19.5 ±â€¯1.9 (61%), 18.1 ±â€¯1.2 (64%), 36.4 ±â€¯3.4 (28%), 13.1 ±â€¯0.8 (74%), 15.6 ±â€¯1.0 (69%), 7.6 ±â€¯0.5 (83%), 11.9 ±â€¯0.8 (76%), 24.7 ±â€¯1.7 (51%) and 32 ±â€¯2.3 (37%) µg mL-1, respectively, over control (50.7 ±â€¯3.6 µg mL-1). A maximum reduction of 8.4 ±â€¯1.2 (46%), 5.8 ±â€¯0.6 (62%) and 4 ±â€¯0.2 (74%) µg mL-1 in 2, 3-DHBA at 300 (1×), 600 (2×) and 900 (3×) µg mL-1 glyphosate, respectively, While, 32.8 ±â€¯2.7 (19%), 27.2 ±â€¯2 (33%) and 21.5 ±â€¯1.3 (47%) µg mL-1, respectively in the production of SA was observed at 300 (1×), 600 (2×) and 900 (3×) µg mL-1 atrazine, respectively. Likewise, with increase in concentration of pesticides, decrease in P solubilization ability and change in pH of broth was detected. The order of pesticide toxicity to PSE (percent decline over control) at highest concentration was: atrazine (45) > kitazin (44) > metalaxyl (43) > monocrotophos (43) > glyphosate (41) > hexaconazole (39) > quizalofop (33) > imidacloprid (31) > fipronil (25). The present study undoubtedly suggests that even at higher doses of pesticides, A. vinelandii maintained secreting plant growth regulators and this property makes this strain agronomically important microbe for enhancing the growth of plants.

A systems-level approach for investigating organophosphorus pesticide toxicity.

The full understanding of the single and joint toxicity of a variety of organophosphorus (OP) pesticides is still unavailable, because of the extreme complex mechanism of action. This study established a systems-level approach based on systems toxicology to investigate OP pesticide toxicity by incorporating ADME/T properties, protein prediction, and network and pathway analysis. The results showed that most OP pesticides are highly toxic according to the ADME/T parameters, and can interact with significant receptor proteins to cooperatively lead to various diseases by the established OP pesticide -protein and protein-disease networks. Furthermore, the studies that multiple OP pesticides potentially act on the same receptor proteins and/or the functionally diverse proteins explained that multiple OP pesticides could mutually enhance toxicological synergy or additive on a molecular/systematic level. To the end, the integrated pathways revealed the mechanism of toxicity of the interaction of OP pesticides and elucidated the pathogenesis induced by OP pesticides. This study demonstrates a systems-level approach for investigating OP pesticide toxicity that can be further applied to risk assessments of various toxins, which is of significant interest to food security and environmental protection.

Levels of organochlorine pesticides in Amazon turtle (Podocnemis unifilis) in the Xingu River, Brazil.

Due to the toxicity and high environmental persistence of organochlorine pesticides in aquatic organisms, turtles have been studied as environment biomonitors. These animals are important sources of protein for the riverside and indigenous peoples of the Brazilian amazon. In the present study, organochlorine pesticide contamination was investigated in Podocnemis unifilis. Liver, muscle and fatty tissue samples were removed from 50 specimens collected from five sampling points located in the Xingu River basin. Fourteen organochlorine pesticides were analysed via gas chromatography with an electron capture detector (CG-ECD). Eight organochlorine pesticides were detected with average concentrations of ∑DDT, ∑Endossulfan and ∑HCH which were 26.17 ± 26.35, 14.38 ± 23.77 and 1.39 ± 8.46 ng g-1 in moisture content, respectively. DDT compounds were the most predominant, with a greater concentration of pp'-DDT in the liver and pp'-DDD in the muscle. Significant differences were noted between the types of tissues studied, and the concentration of OCPs varied between sampling sites.

Effect of seed pre-soaking with 24-epibrassinolide on growth and photosynthetic parameters of Brassica juncea L. in imidacloprid soil.

Pesticides are widely used to protect crop plants from various insect pests. However, application of pesticides causes phytotoxicity to plants which results in their impaired growth and development. Brassinosteroids are well known to protect plants under abiotic stress conditions. The purpose of the present study was to access the ameliorative role of 24-epibrassinolide (EBR) in Brassica juncea L. under imidacloprid (IMI) toxicity. B. juncea plants were raised from seeds soaked in 0.1, 1 and 100nM of EBR, and grown in soils amended with 250, 300 and 350mgkg(-1) IMI pesticide, and observed for growth, pigments and photosynthetic parameters after 30, 60 and 90 days of seed sowing. The plants grown in soil treated with IMI exhibited a significant reduction in shoot length, number of leaves, chlorophyll contents and photosynthetic parameters like photosynthetic rate, stomatal conductance, inter-cellular CO2 and transpiration rate, when compared with their respective controls. However, pigments which act as antioxidants such as carotenoids, anthocyanins and xanthophylls were increased with IMI stress. Pre-sowing seed treatment with EBR decreased the toxic effects of IMI and increased the growth, pigment biosynthesis and photosynthetic parameters of the plants grown in IMI amended soil. Maximum increase in all the growth and photosynthetic parameters was noticed in plants raised from seeds treated with 100nM EBR and grown in IMI amended soil.

Current view of zinc as a hepatoprotective agent in conditions of chlorpyrifos induced toxicity.

Human population bears the brunt of deadly hepatotoxic, neurodegenerative, behavioural and various other developmental disorders due to pesticide toxicity through environmental or occupational exposures. The application of pesticides to control pests in land and water has posed potential health hazards to live stock and wildlife including fishes, mammals, birds and humans. Therefore, various scientific approaches are being considered to tackle the problem of pesticide poisoning especially in developing economies. The role of essential trace elements as the promising and efficient preventive prophylactic agents without any toxicity and side effects in attenuating the adverse effects caused by pesticides, have been reported by various scientists, the world over. In this perspective, zinc, a key constituent of more than 300 mammalian enzymes and many transcription factors has proved its protective potential in various models of animal toxicity. The hepato-protective potential of zinc has been proved during various toxic states including pesticide toxicity. However, zinc warrants further examination with regard to documentation of specific molecular pathways to establish the exact mechanisms for zinc-mediated protection during pesticide toxicity.

Malathion exposure may increase infertility risk among US Adults: Results from the 2015-2016 NHANES.

BACKGROUND AND PURPOSE: Organophosphate pesticides such as malathion are the most widely used pesticides. Despite endocrine-disrupting effects, there is a paucity of information regarding chronic exposure to non-persistent organopesticides such as malathion. The purpose of this study is to describe the exposure burden among U.S. residents as well as possible impacts on fertility. METHODS: Population-based data collected by the National Health and Nutrition Examination Survey (NHANES) between 2015 and 2016 were used to perform a retrospective analysis on urinary concentrations of malathion diacid. Samples were assessed from 1703 adult participants, statistically weighted to represent over 231 million individuals. General linear models were used to examine associations between exposure and reproductive health measures among pre-menopausal women. RESULTS: Detectable concentrations of malathion diacid were identified in 16.1 % (n = 254) of samples. Concentrations were higher among women who reported seeing a physician due to difficulties becoming pregnant (P < 0.001; r2 = 0.12) as well as among women who reported trying for at least a year to become pregnant (P < 0.001; r2 = 0.06). CONCLUSIONS: Exposure to malathion is associated with a history of reproductive health challenges among women.

Multiple lines of evidence point to pesticides as stressors affecting invertebrate communities in small streams in five United States regions.

Multistressor studies were performed in five regions of the United States to assess the role of pesticides as stressors affecting invertebrate communities in wadable streams. Pesticides and other chemical and physical stressors were measured in 75 to 99 streams per region for 4 weeks, after which invertebrate communities were surveyed (435 total sites). Pesticides were sampled weekly in filtered water, and once in bed sediment. The role of pesticides as a stressor to invertebrate communities was assessed by evaluating multiple lines of evidence: toxicity predictions based on measured pesticide concentrations, multivariate models and other statistical analyses, and previously published mesocosm experiments. Toxicity predictions using benchmarks and species sensitivity distributions and statistical correlations suggested that pesticides were present at high enough concentrations to adversely affect invertebrate communities at the regional scale. Two undirected techniques-boosted regression tree models and distance-based linear models-identified which pesticides were predictors of (respectively) invertebrate metrics and community composition. To put insecticides in context with known, influential covariates of invertebrate response, generalized additive models were used to identify which individual pesticide(s) were important predictors of invertebrate community condition in each region, after accounting for natural covariates. Four insecticides were identified as stressors to invertebrate communities at the regional scale: bifenthrin, chlordane, fipronil and its degradates, and imidacloprid. Fipronil was particularly important in the Southeast region, and imidacloprid, bifenthrin, and chlordane were important in multiple regions. For imidacloprid, bifenthrin, and fipronil, toxicity predictions were supported by mesocosm experiments that demonstrated adverse effects on naïve aquatic communities when dosed under controlled conditions. These multiple lines of evidence do not prove causality-which is challenging in the field under multistressor conditions-but they make a strong case for the role of insecticides as stressors adversely affecting invertebrate communities in streams within the five sampled regions.

Transferring knowledge across aquatic species via clustering techniques to unravel patterns of pesticide toxicity.

In silico modelling takes the advantage of accelerating ecotoxicological assessments on hazardous chemicals without conducting risky in vivo experiments under ethic regulation. To date, the prevailing strategy of one model for one species cannot be well generalized to multi-species modelling. In this work, we propose a new strategy of one model for multiple species to facilitate knowledge transfer across aquatic species. The available lethal concentration values of 4952 pesticides on 651 fish species are aggregated into one toxicity response matrix, purely through which we attempt to unravel fish toxicosis-phylogenesis relationships and pesticide toxicity-structure relationships via clustering techniques including non-negative matrix factorization (NMF) and hierarchical clustering. The clustering results suggest that (1) close NMF weights indicate close species-toxicosis and pesticide-toxicity profiles; (2) and that species toxicosis patterns are related with species phylogenetic relationships; (3) and that close pesticide-toxicity profiles indicate similar atom-pair structural fingerprints. These environmental, chemical and biological insights can be used as expert knowledge for environmentalists to manually gain knowledge about untested species/pesticides from tested species/pesticides, and meanwhile provide support for us to build in silico models from species phylogenetic and pesticide structural points of view. Besides unravelling the mechanisms behind toxicity response, we also adopt stratified cross validation and external test to validate the reliability of using NMF to predict missing toxicity values. Independent test on external data shows that NMF achieves 0.8404-0.9397 R2 on four fish species. In the context of toxicity prediction, non-negative matrix factorization can be viewed as a model based on quantitative activity-activity relationships (QAAR), and provides an alternative approach of inferring toxicity values on untested species from tested species.

Characterization of the dose decrement in regulatory rat pesticide toxicity feeding studies.

In typical rodent pesticide feeding studies of 4 up to 104 weeks, animals are offered the pesticide at constant concentrations in the feed. Throughout the entire study duration of up to 104 weeks, the daily feed consumption per animal remains nearly constant. This results in decreasing doses per kg bodyweight from the first day of treatment onwards as the bodyweight increases. Recently, we have identified this dose decrement as the major cause for lower No Observed Effect Levels (NOAEL, expressed as mg/kg bodyweight) in longer-term studies compared to shorter-term studies, rather than the exposure duration itself. In the current evaluation we investigated the nature of the dose decrement in more detail by using male and female bodyweight and feed consumption data from 118 feeding studies of three rat strains to calculate dose development over time. In male rats, after a steep initial dose decrement, the mean dose at week 7 of treatment is on average half of the initial dose and after 29 weeks one third of the initial dose. In females, 50% of the initial dose is reached at week 18 and in 25% of the studies one third of the initial dose is reached at approximately 75 weeks of treatment. Although bodyweights and feed intakes per animal were different between strains, doses and dose development curves over time are similar. The fact that ingested doses in rats continually decrease, especially in the first 13 weeks, should be taken into account in dietary risk assessments.

Global warming and glyphosate toxicity (II): Offspring zebrafish modelling with behavioral, morphological and immunohistochemical approaches.

The increase in temperature due to global warming greatly affects the toxicity produced by pesticides in the aquatic ecosystem. Studies investigating the effects of such environmental stress factors on next generations are important in terms of the sustainability of ecosystems. In this study, the effects of parental synergistic exposure to glyphosate and temperature increase on the next generation were investigated in a zebrafish model. For this purpose, adult zebrafish were exposed to 1 ppm and 5 ppm glyphosate for 96 h at four different temperatures (28.5, 29.0, 29.5, 30.0 °C). At the end of this period, some of the fish were subjected to the recovery process for 10 days. At the end of both treatments, a new generation was taken from the fish and morphological, physiological, molecular and behavioral analysis were performed on the offspring. According to the results, in parallel with the 0.5-degree temperature increase applied to the parents with glyphosate exposure, lower survival rate, delay in hatching, increased body malformations and lower blood flow and heart rate were detected in the offspring. In addition, according to the results of whole mouth larva staining, increased apoptosis, free oxygen radical formation and lipid accumulation were detected in the offspring. Moreover, it has been observed that the temperature increases to which the parents are exposed affects the light signal transmission and serotonin pathways in the offspring, resulting in more dark/light locomotor activity and increased thigmotaxis.

Increased bat hunting at polluted streams suggests chemical exposure rather than prey shortage.

Streams and their riparian areas are important habitats and foraging sites for bats feeding on emergent aquatic insects. Chemical pollutants entering freshwater streams from agricultural and wastewater sources have been shown to alter aquatic insect emergence, yet little is known about how this impacts insectivorous bats in riparian areas. In this study, we investigate the relationships between the presence of wastewater effluent, in-stream pesticide toxicity, the number of emergent and flying aquatic insects, and the activity and hunting behaviour of bats at 14 streams in southwestern Germany. Stream sites were located in riparian forests, sheltered from direct exposure to pollutants from agricultural and urban areas. We focused on three bat species associated with riparian areas: Myotis daubentonii, M. cf. brandtii, and Pipistrellus pipistrellus. We found that streams with higher pesticide toxicity and more frequent detection of wastewater also tended to be warmer and have higher nutrient and lower oxygen concentrations. We did not observe a reduction of insect emergence, bat activity or hunting rates in association with pesticide toxicity and wastewater detections. Instead, the activity and hunting rates of Myotis spp. were higher at more polluted sites. The observed increase in bat hunting at more polluted streams suggests that instead of reduced prey availability, chemical pollution at the levels measured in the present study could expose bats to pollutants transported from the stream by emergent aquatic insects.