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Radiophotothermal therapy is a promising treatment for superficial tumors. Traditional radiotherapy requires tissue boluses on the patient's skin to increase therapeutic effectiveness due to the dose-buildup effect of high-energy radiation. However, combining radiotherapy with photothermal therapy leads to uncertainties as the low-penetration near-infrared light dose is reduced after penetrating the bolus. To enhance precision and effectiveness, this study introduces a novel bolus made of AuNPs@poly(AM-THMA-DMAEMA) composite hydrogel. This hydrogel is prepared through a one-pot method involving the reduction of trihydrate chloroauric acid (HAuCl4·3H2O) and copolymerization of acrylamide (AM) and N-[Tris(hydroxymethyl)methyl]acrylamide (THMA) in a redox system with dimethylaminoethyl methacrylate (DMAEMA) and potassium persulfate (KPS). The gold nanoparticles (AuNPs) improve the mechanical strength (tensile strength of 320.84 kPa, elongation at break of 830%) and antibacterial properties (>99% against Staphylococcus aureus). The local surface plasmon resonance (LSPR) effect of AuNPs enables the hydrogel to absorb near-infrared light for precise monitoring of the infrared radiation dose. The hydrogel's biocompatibility is enhanced by the absence of additional crosslinking agents, and its excellent surface adhesion strength is due to numerous hydrogen bonds and electrostatic interactions. This study offers new possibilities for nanoparticle composite hydrogels as tissue boluses, achieving high precision and efficiency in radiophotothermal therapy.
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The McMurry coupling is a facile, gentle and low-cost chemical reaction for synthesizing. Here, for the first time, we employed the McMurry coupling reaction to prepare π-conjugated anion exchange membranes (AEMs). The inter-chain π-π stacking between adjacent benzene rings induces directional self-assembly aggregation and enables highly ordered ion-conductive channels. The resulting structure was characterized through UV/VIS spectrum, X-ray diffraction (XRD) pattern, small-angle X-ray scattering (SAXS), transmission electron microscopy (TEM) and density functional theory (DFT) calculations, leading to high OH- conductivity of 135.5â mS cm-1 at 80 °C. Furthermore, the double bonds in the π-conjugated system also trigger in situ self-crosslinking of the AEMs to enhance dimensional and alkaline stability. Benefiting from this advantage, the as-obtained Cr-QPPV-2.51 AEM exhibits superior alkaline stability (95 % conductivity retention after 3000â hrs in 1â M KOH at 80 °C) and high mechanical strength of 34.8â MPa. Moreover, the fuel cell using Cr-QPPV-2.51 shows a maximum peak power density of 1.27â W cm-2 at 80 °C.
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The fabrication of block copolymer (BCP) vesicles with controlled membrane permeability and promising stability remains a considerable challenge. Herein, a new type of pH-responsive and self-crosslinked vesicle based on a hydrolytically hindered urea bond is reported. This kind of vesicle is formed by the self-assembly of a pH-responsive and hydrolytically self-crosslinkable copolymer poly(ethylene glycol)-block-poly[2-(3-(tert-butyl)-3-ethylureido)ethyl methacrylate-co-2-(diethylamino)ethyl methacrylate] (PEG-b-P(TBEU-co-DEA)). The BCP can be easily synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization of 2-(3-(tert-butyl)-3-ethylureido)ethyl methacrylate (TBEU) and 2-(diethylamino)ethyl methacrylate (DEA) using PEG-based macro-chain transfer agent. The copolymer could self-assemble into stable vesicles by the hydrophobic interaction and in situ cross-linking between amines and isocyanates after the hydrolysis of the hindered urea bonds without any catalyst. Dynamic light scattering (DLS) studies show that the vesicles exhibit enhanced stability against the dilution of organic solvent, and the size can be adjusted through the change of pH values. Moreover, the alkaline phosphatase-loaded vesicles can act as nano-reactor and enable free diffusion of small molecules into the vesicles, followed by the significantly improved fluorescence intensity of phosphate-caged fluorescein. This self-crosslinking and pH-sensitive vesicles may serve as a smart platform in controlled drug delivery and molecular reactor.
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Reagentes de Ligações Cruzadas/síntese química , Polímeros/síntese química , Reagentes de Ligações Cruzadas/química , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Estrutura Molecular , Tamanho da Partícula , Polímeros/química , Propriedades de SuperfícieRESUMO
Hemostatic powders that adapt to irregularly shaped wounds, allowing for easy application and stable storage, have gained popularity for first-aid hemorrhage control. However, traditional powders often provide weak thrombus support and exhibit limited tissue adhesion, making them susceptible to dislodgment by the bloodstream. Inspired by fibrin fibers coagulation mediator, we have developed a bi-component hemostatic powder composed of positively charged quaternized chitosan (QCS) and negatively charged catechol-modified alginate (Cat-SA). Upon application to the wound, the bi-component powders (QCS/Cat-SA) rapidly absorb plasma and dissolve into chains. These chains interact with each other to form a network, which can effectively bind and entraps clustered red blood cells and platelets, ultimately leading to the creation of a durable and robust thrombus. Significantly, these interconnected polymers adhere to the injury site, offering protection against thrombus disruption caused by the bloodstream. Benefiting from these synthetic properties, QCS/Cat-SA demonstrates superior hemostatic performance compared to commercial hemostatic powders like Celox™ in both arterial injuries and non-compressible liver puncture wounds. Importantly, QCS/Cat-SA exhibits excellent antibacterial activity, cytocompatibility, and hemocompatibility. These advantages of QCS/Cat-SA, including strong blood clotting, wet tissue adherence, antibacterial activity, biosafety, ease of use, and stable storage, make it a promising hemostatic agent for emergency situations.
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Quitosana , Hemostáticos , Trombose , Humanos , Fibrina , Adesivos/farmacologia , Coagulação Sanguínea , Hemostáticos/farmacologia , Quitosana/farmacologia , Polissacarídeos/farmacologia , Antibacterianos/farmacologiaRESUMO
Injectable hydrogels have wide applications in clinical practice. However, the development of tough and bioadhesive ones based on biopolymers, along with biofriendly and robust crosslinking strategies, still represents a great challenge. Herein, we report an injectable hydrogel composed of maleimidyl alginate and pristine gelatin, for which the precursor solutions could self-crosslink via mild Michael-type addition without any catalyst or external energy upon mixing. This hydrogel is tough and bioadhesive, which can maintain intactness as well as adherence to the defect of porcine skin under fierce bending and twisting, warm water bath, and boiling water shower. Besides, it is biocompatible, bioactive and biodegradable, which could support the growth and remodeling of cells by affording an extracellular matrix-like environment. As a proof of application, we demonstrate that this hydrogel could significantly accelerate diabetic skin wound healing, thereby holding great potential in healthcare.
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Materiais Biocompatíveis , Gelatina , Animais , Suínos , Materiais Biocompatíveis/farmacologia , Hidrogéis , Alginatos , ÁguaRESUMO
The pathological changes in inflammatory bowel disease (IBD) include the disruption of intestinal barrier function and the infiltration of pathogenic microbes. The application of an artificial protective barrier at the site of inflammation can prevent bacterial infiltration, promote epithelial cell migration, and accelerate wound healing. In this study, dopamine-modified hyaluronic acid (HA-DA) was developed as a bioadhesive self-cross-linkable hydrogel, which acted as an enteroprotective agent to promote the healing of inflamed intestinal tissue. The adhesion strength HA-DA to mouse colon was 3.81-fold higher than HA. Moreover, HA-DA promoted Caco-2 cell proliferation and migration as well as had a strong physical barrier effect after gelation. After oral administration, the HA-DA reduced weight loss and attenuated impaired goblet cell function in mice with dextran sodium sulfate-induced IBD. In addition, HA-DA promoted restoration of the epithelial barrier by the upregulation of tight junction proteins. The results reported herein substantiated that self-cross-linkable hydrogel-based enteroprotective agents are a promising approach for the treatment of IBD.
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Ácido Hialurônico , Hidrogéis , Doenças Inflamatórias Intestinais , Ácido Hialurônico/química , Ácido Hialurônico/farmacologia , Animais , Humanos , Doenças Inflamatórias Intestinais/tratamento farmacológico , Doenças Inflamatórias Intestinais/patologia , Camundongos , Células CACO-2 , Hidrogéis/química , Hidrogéis/farmacologia , Proliferação de Células/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Substâncias Protetoras/química , Movimento Celular/efeitos dos fármacos , Masculino , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/metabolismo , Sulfato de DextranaRESUMO
Skin wound dressings are commonly utilized for the treatment of skin injuries, as they effectively facilitate wound healing and possess anti-inflammatory and antibacterial properties. However, conventional dressings fail to inhibit ROS production and promote vascularization, leading to delayed wound healing. Here, we developed injectable self-crosslinking hydrogels through thiolated hyaluronic acid (HASH/rhCOLIII) with enhancing the ROS inhibitory capacity while preserving the cell adhesion ability of hyaluronic acid. Additionally, recombinant humanized collagen type III (rhCOLIII) is incorporated via electrostatic adsorption to further enhance mechanical strength and angiogenesis properties of the hydrogel. The HASH/rhCOLIII demonstrated excellent biocompatibility, remarkable ROS scavenging ability, as well as hemostatic and angiogenic properties. Cell experiment results show that HASH/rhCOLIII has excellent biocompatibility and can significantly promote angiogenesis. Animal experiments results showed that HASH/rhCOLIII exhibits anti-inflammatory effects, significantly accelerating wound healing in a full-thickness skin defect model. These findings highlight that HASH/rhCOLIII hydrogel holds great promise as an advanced dressing for effective wound healing.
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The escalating global demand for sustainable manufacturing, motivated by concerns over energy conservation and carbon footprints, encounters challenges due to insufficient renewable materials and arduous fabrication procedures to fulfill specific requirements in medical and healthcare systems. Here, biosafe pollen cryogel is engineered as effective hemostats without additional harmful crosslinkers to treat deep noncompressible wounds. A straightforward and low-energy approach is involved in forming stable macroporous cryogel, benefiting from the unique micro-hierarchical structures and chemical components of non-allergenic plant pollen. It is demonstrated that the pollen cryogel exhibits rapid water/blood-triggered shape-memory properties within 2 s. Owing to their inherent nano/micro hierarchical structure and abundant chemical functional groups on the pollen surface, the pollen cryogel shows effective hemostatic performance in a mouse liver penetration model, which is easily removed after usage. Overall, the self-crosslinking pollen cryogel in this work pioneers a framework of potential clinical applications for the first-hand treatment on deep noncompressible wounds.
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The modeling of polymeric reactions is a topic of large interest. The gelation reactions that may result from self-crosslinking or hybrid (agent based-) crosslinking are examples with interest specially in biomaterials applications. The composition of polymer entities during the reaction is hard to follow, and their concentration is not a good measure of the system dynamics. One alternative is monitoring the rheological behavior of the reacting mass, and relate the elastic modulus of the mixture with the rheological degree of conversion. In this paper we use rheological data to fit Malkin and Kulichikin (1996) [1] based models to describe the crosslinking of chitosan. First, the self-crosslinking of chitosan is considered. Then, the agent-based crosslinking reaction promoted by genipin is addressed. We use dynamical rheological data to fit the reaction models. The model fitting problem generated using Maximum Likelihood principle with heteroscedastic prediction error variance is formulated as a Dynamic Optimization problem and subsequently solved with a sequential approach. Parametric confidence regions are computed using the linear approximation of the covariance matrix at the optimum. Further, the parameters correlation matrix is also determined and used to qualitatively infer about the practical identifiability. The reaction order obtained for self-crosslinking kinetics is 1.3375 ± (0.0151) - approximately of first order -, and is 2.2402 ± (0.0373) for hybrid crosslinking (approximately of second order). In both cases we prove the error variance model is heteroskedastic and the model is identifiable. The approach proposed herein can be extended to other polymer systems.
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Quitosana , Reologia , Polímeros , CinéticaRESUMO
Derivatives [i.e. proteins and exopolysaccharides (EPS)] from Lactobacillus delbrueckii subsp. bulgaricus (LB) were extracted, characterized, and for the first time used in the production of novel self-crosslinking 3D printed alginate/hyaluronic acid (ALG/HA) hydrogels, as high-value functional biomaterials with therapeutic potentials in regenerative medicine applications. Derivatives coming from two different LB strains, LB1865 and LB1932, were tested in-vitro and compared for their cytotoxicity and effect on proliferation and migration on human fibroblast. EPS received particular attention as showing relevant dose-dependent cytocompatibility against the human fibroblast. The derivatives showed an ability to increase cell proliferation and migration, quantifiable between 10 and 20 % if compared to controls, with higher values for the derivatives obtained from the LB1932 strain. These were explained by liquid chromatography-mass spectrometry targeted protein biomarker analysis as a decrease in matrix-degrading and proapoptotic proteins, associated with an increase in collagen and antiapoptotic proteins production. LB1932 enriched hydrogel was found to be of benefit compared to control dressings, giving the more promising results as potential for in vivo skin wound healing tests.
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Lactobacillus delbrueckii , Humanos , Lactobacillus delbrueckii/metabolismo , Ácido Hialurônico/metabolismo , Hidrogéis/farmacologia , Hidrogéis/metabolismo , Cicatrização , Impressão TridimensionalRESUMO
Hemostatic powders with flexible shape are widely used for the noncompressible and inaccessible hemorrhage wounds. However, current hemostatic powders display poor wet tissue adhesion and fragile mechanical strength of the powder-supported blood clots, leading to compromised hemostasis efficacy. Herein, a bi-component of carboxymethyl chitosan (CMCS) and aldehyde-modified hyaluronic acid grafted with catechol groups (COHA) was designed. Upon absorption of blood, the bi-component powders (CMCS-COHA) spontaneously self-crosslinks into an adhesive hydrogel within 10 s, tightly adhering to wound tissue to form a pressure-resistant physical barrier. During gelation, the hydrogel matrix captures and locks the blood cells/platelets to generate a robust thrombus in the bleeding sites. Compared with traditional hemostatic powder Celox™, CMCS-COHA displays superior blood coagulation and hemostatic performance. More importantly, CMCS-COHA has inherent cytocompatibility and hemocompatibility. These prominent advantages in rapid and effective hemostasis, adaptability to fit irregulate defective wound, easy preservation, facile usage, and bio-safety, make CMCS-COHA a promising hemostatic in emergency situations.
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Quitosana , Hemostáticos , Adesivos Teciduais , Humanos , Pós/farmacologia , Adesivos Teciduais/farmacologia , Hemostasia , Hemostáticos/farmacologia , Quitosana/farmacologia , Polissacarídeos/farmacologia , Hemorragia/tratamento farmacológico , Hidrogéis/farmacologiaRESUMO
Quasi-dry CO2 fracturing technology is a new CO2 fracturing technology that combines liquid CO2 fracturing (dry CO2 fracturing) and water-based fracturing. It uses a liquid CO2 system containing a small amount of water-based fracturing fluid to carry sand, and it is characterized by sand blending at normal pressure, convenient preparation, the integrated application of resistance reduction and sand carrying, and no dedicated closed sand blender requirement. We developed a self-crosslinking emulsion-type water-based fracturing fluid (ZJL-1), which contained ionic bonds, hydrogen bonds, van der Waals forces, and hydrophobic associations, for quasi-dry CO2 fracturing, and the comprehensive properties of the ZJL-1 fracturing fluid were evaluated. The results showed that the ZJL-1 fracturing fluid had obvious viscoelastic characteristics, a heat loss rate of less than 10% at 200 °C, a good thermal stability, sufficient rheology under high temperature and high shear conditions, and a good thermal stability. The resistance reduction rate reached 70%, which demonstrates a good resistance reduction performance. Compared with conventional guar fracturing fluid, ZJL-1 can carry more sand and has a lower core damage rate. The on-site use of quasi-dry fracturing showed that optimizing the mixing ratio of liquid CO2 fracturing fluid and ZJL-1 fracturing fluid effectively enhanced oil and gas recovery. This can be used to optimize quasi-dry fracturing and can be used as a reference.
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A transdermal patch that delivers insulin at high glucose concentrations can offer tremendous advantages to ease the concern of safety and improve the quality of life for people with diabetes. Herein, a novel self-crosslinkable and glucose-responsive polymer-based microneedle patch (MN) is designed to deliver insulin at hyperglycemia. The microneedle patch is made of hyaluronic acid polymers functionalized with dopamine and 4-amino-3-fluorophenylboronic acid (AFBA) that can be quickly crosslinked upon mixing of the polymer solutions in the absence of any chemicalcrosslinking agents or organic solvents. The catechol groups in the dopamine (DA) units form covalent crosslinkages among themselves by auto-oxidation and dynamic crosslink with phenylboronic acid (PBA) via complexation. The reversible crosslinkages between catechol and boronate decrease with increasing glucose concentration leading to higher swelling and faster insulin release at hyperglycemia as compared to euglycemia. Such superior glucose-responsive properties are demonstrated by in vitro analyses and in vivo efficacy studies. The hydrogel polymers also preserve native structure and bioactivity of insulin, attributable to the interaction of hyaluronic acid (HA) with insulin molecules, as revealed by experiments and molecular dynamics simulations. The simplicity in the design and fabrication process, and glucose-responsiveness in insulin delivery impart the matrix microneedle (mMN) patch great potential for clinical translation.
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Diabetes Mellitus Experimental , Hiperglicemia , Animais , Humanos , Insulina/química , Glicemia/análise , Ácido Hialurônico/química , Polímeros/química , Dopamina , Qualidade de Vida , Diabetes Mellitus Experimental/tratamento farmacológico , GlucoseRESUMO
H2O2 is essential for cellular processes and plays a vital role in the regulation of cell signaling pathways, which can be viewed as a warning signal for many kinds of disease including cancer, cardiovascular disease, reproductive abnormalities, diabetes, and renal failure. A H2O2-responsive hydrogel (H2O2-Gel) is a promising candidate for biomedical applications because of its good biocompatibility, similarity to soft biological tissues, ease of preparation, and its ability to respond to H2O2. In this study, the H2O2-responsive moieties used to fabricate H2O2-Gels were reviewed, including thioethers, disulfide bonds, selenides, diselenium bonds, diketones, boronic, and others. Next, the preparation method of H2O2-Gel was divided into two major categories according to their reaction mechanisms: either self-crosslinking or mechanisms entailing the addition of difunctional crosslinkers. Last, the applications of H2O2-Gels were emphasized, which have been viewed as desirable candidates in the fields of drug delivery, the detection of H2O2, glucose-responsive systems, ROS scavengers, tissue engineering, and cell-encapsulation.
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Cellulose/chitosan composite, as a mature commercial antibacterial dressing, is an important type of wound repair material. However, how to achieve the perfect compound of two components and improve antibacterial activity is a major, lingering issue. In this study, a bifunctional group modified bacterial cellulose (DCBC) was prepared by carboxymethylation and selective oxidation. Further, the chitosan (CS) was compounded in the network of DCBC by self-crosslinking to form dialdehyde carboxymethyl bacterial cellulose/chitosan composites (S-DCBC/CS). The aldehyde group can react with amino of CS by Schiff base reaction. The carboxyl group of DCBC and the amorphous distribution of CS molecular chains increase the antimicrobial properties of composites. The bacteriostatic rate of composites could be higher than 95%. Bacteria can be attracted onto the surface of composites, what we call it "directional adhesion antibacterial effects". In particular, a kind of large animal wound model, deep â ¡ degree infected scald of Bama miniature pig, was used to research the antimicrobial and healing properties of materials. The S-DCBC/CS can effectively inhibit bacterial proliferation of wound and kill the bacteria. The wound healing rate of S-DCBC/CS was up to 80% after three weeks. The composites show better antibacterial and promoting concrescence effects than traditional chitosan dressings.
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Premna microphylla turcz leaves (PMTL) is a resource-rich, biodegradable, renewable biomass. Here, a microsphere adsorbent was prepared from PMTL by a self-crosslinking method without any addition of chemical cross-linking agent, and characterized by SEM, FTIR, and XPS. The influence of preparation methods and conditions on the properties of the microspheres was studied and the self-crosslinking mechanism was analyzed. The effects of temperature, pH, contact time, uranium concentration, and adsorbent dosage on its adsorption performance toward to uranium were systematically explored. The results showed that PMTL endogenous pectin binding with endogenous Ca2+, Mg2+ and other metal ions to form an 'egg box' structure might be the mechanism of its self-crosslinking to form microspheres. The adsorption isotherms fitted well by the Freundlich model and the experimental maximum adsorption capacity of microspheres was 346.65 mg·g-1 at pH of 5, and kinetics data correlated well with the pseudo-second order model. The adsorption mechanism might be the coordination bonding between the uranium and oxygen-containing groups (hydroxyl and carboxyl groups), and the ion exchange between the uranium and metal ions (mainly Ca2+ and Mg2+). The PMTL microspheres are promising in treating uranium-containing wastewater in a more cost-effective and environmentally friendly manner.
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Urânio , Adsorção , Concentração de Íons de Hidrogênio , Cinética , Microesferas , Folhas de PlantaRESUMO
With increasing interest in aging and skin care, the use of fillers to increase the volume of soft tissue volume is increasing globally. However, the side effects caused by the residual chemical crosslinking agents present in these fillers limit the effective application of commercialized filler products. Therefore, the development of a novel crosslinking system with a non-toxic chemical crosslinking agent is required to overcome the limitations of commercial hyaluronate (HA)-based fillers. In this paper, a new injectable hydrogel with enhanced mechanical properties, tissue adhesion, injectability, and biocompatibility is reported. The HA derivatives modified with catechol groups (HA-DA) were crosslinked by self-oxidation under in vivo physiological conditions (pH 7.4) without chemical crosslinkers to form hydrogels, which can be further accelerated by the dissolved oxygen in the body. The fabricated HA-DA filler showed excellent mechanical properties and could be easily injected with a low injection force. Further, the HA-DA filler stably attached to the injection site due to the tissue adhesion properties of the catechol groups, thus leading to an improved displacement stability. In addition, the HA-DA filler showed excellent cell viability, cell proliferation, and biocompatibility. Therefore, the HA-DA hydrogel is a novel soft tissue filler with great potential to overcome the limitations of commercial soft tissue fillers.
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Preenchedores Dérmicos/síntese química , Ácido Hialurônico/administração & dosagem , Hidrogéis/síntese química , Animais , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Preenchedores Dérmicos/química , Ácido Hialurônico/química , Ácido Hialurônico/farmacologia , Hidrogéis/química , Concentração de Íons de Hidrogênio , Injeções , Masculino , Camundongos , Células NIH 3T3RESUMO
Fabrication of simple and efficient adsorbents is greatly vital to satisfy the requirements of removal of tellurium in wastewater treatment, yet remains challenging. Here, a facile and cost-effective strategy to develop ZIF-L coated self-crosslinking cellulose membrane (ZIF-L/SC membrane) for tellurium adsorption was presented. In-situ vertical growth of ZIF-L nanoplates with functional properties on membrane substrate is an available strategy, effectively remedying deficiency of pure nanosized sorbent in agglomeration problem and unhandy recovery. The SC membrane formed by strong hydrogen bonding among cellulose fibers is an excellent substrate, due to the favorable mechanical strength and abundant hydroxyl groups. The as-prepared ZIF-L/SC membrane shows advantageous morphology of large contact surface, fine thermal stability and eligible mechanical strength. The adsorption performance and possible mechanism of ZIF-L/SC membrane for Te (IV) were investigated by diverse characterization methods, showing admirable adsorption effect. Furthermore, the ZIF-L/SC membrane has excellent antibacterial properties, thus it is expected to deal with membrane fouling caused by microorganism breeding. Therefore, the bifunctional ZIF-L/SC membrane with excellent antibacterial activity is anticipated to be a promising candidate for efficient tellurium adsorbents, and simultaneously have potential in various fields in the future.
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Zeolitas , Adsorção , Antibacterianos/farmacologia , Celulose , TelúrioRESUMO
HYPOTHESIS: The application of ferritin containers as a promising drug delivery vehicle is limited by their low bioavailability in blood circulation due to unfavorable environments, such as degradation by protease. The integration of ferritin containers into the polymeric network of microgels through electrostatic interactions is expected to be able to protect ferritin against degradation by protease. Furthermore, a stimuli-responsive microgel system can be designed by employing an acid-degradable crosslinker during the microgel synthesis. This should enable ferritin release in an acidic environment, which will be useful for future drug delivery applications. EXPERIMENTS: Nanoparticle/fluorophores-loaded ferritin was integrated into microgels during precipitation polymerization. The integration was monitored by transmission electron microscopy (TEM)2 and fluorescence microscopy, respectively. After studying ferritin release in acidic solutions, we investigated the stability of ferritin inside microgels against degradation by chymotrypsin. FINDINGS: About 80% of the applied ferritin containers were integrated into microgels and around 85% and 50% of them could be released in buffer pH 2.5 and 4.0, respectively. Total degradation of the microgels was not achieved due to the self-crosslinking of N-isopropylacrylamide (NIPAM). Finally, we prove that microgels could protect ferritin against degradation by chymotrypsin at 37 °C.