Oral administration of PEDV-dissolved Alg-CS gel induces high and sustained mucosal immunity in mice.

Porcine epidemic diarrhea (PED) is a serious disease in piglets that leads to high mortality. An effective measure that provides higher IgA levels in the intestine and milk is required to decrease losses. Porcine epidemic diarrhea virus (PEDV) was dissolved in calcium alginate (Alg) and combined with chitosan (CS) via electrostatic interactions between cationic chitosan and anionic alginate to create a porous gel (Alg-CS+PEDV). The gel was used to immunize mice orally or in combination with subcutaneous injections of inactivated PEDV vaccine. At 12 and 24 days after immunization, levels of IgA and IgG in Alg-CS+PEDV were higher than with normal PEDV oral administration. At 24 days after immunization, the concentration of IFN-γ in Alg-CS+PEDV was higher than with normal PEDV oral administration. Furthermore, oral administration combining subcutaneous immunization induced higher levels of IgG and IgA than oral administration alone. Our study provides a new method for the preparation and administration of oral vaccines to achieve enhanced mucosal immunity against PEDV.

Efficacy and safety of MC-003 solution for endoscopic mucosal or submucosal resection: a prospective, multicenter, randomized, triple-blinded, parallel-group, phase III study.

BACKGROUND AND AIMS: The safety and efficacy of solutions for submucosal injection are critical for endoscopic resection of gastric adenomas or early gastric cancers. Although several injectable solutions have been introduced for endoscopic resection, they have some limitations. We aimed to compare the efficacy of the new sodium alginate-based solution MC-003 with that of normal saline (NS; 0.9% sodium chloride). METHODS: In this randomized, triple-blind study, 70 patients were initially enrolled for EMR or endoscopic submucosal dissection (ESD). The main outcomes included the need for additional injections, completion of en bloc resection, and occurrence of adverse events. RESULTS: Each group ultimately included 34 patients. Complete en bloc resections were achieved in all patients (P = 1.000). The MC-003 group had more peri-neoplasm tissue fibrosis (P = .056) and needed fewer additional injections for lesions >15 mm (P = .037), located in the distal portion of the stomach (P = .007), and during ESD procedures (P = .001). The adverse event rate was comparable in both groups. CONCLUSIONS: MC-003 outperformed NS in reducing the need for additional injections during en bloc resection, particularly in larger lesions located in the distal portion of the stomach (where most lesions were found) during ESD procedures, without increasing the incidence of serious adverse events. MC-003 is a promising submucosal injectable solution in real-world clinical settings.

Oral quercetin nanoparticles in hydrogel microspheres alleviate high-altitude sleep disturbance based on the gut-brain axis.

High-altitude sleep disturbance is a common symptom of acute mountain sickness, which can be alleviated via modulation of the gut-brain axis. Quercetin (Que) is used to modulate gut microbiota and serves as a potential drug to regulate the gut-brain axis, but the poor solubility and bioavailability affect its biological functions. Here, Que nanoparticles (QNPs) were prepared with zein using an antisolvent method, and QNP-loaded calcium alginate hydrogel microspheres (QNP@HMs) were prepared using electrospinning technology to improve the gastrointestinal stability and intestinal adhesion of QNPs. In the mouse model of high-altitude sleep disturbance, oral administration of QNP@HMs before the mice entering high altitude prolonged sleep duration, improved blood cell recovery, spontaneous behavior and short-term memory, and reduced such inflammation factors as TNF-α and iNOS. Moreover, QNP@HMs enhanced the abundance of probiotics in the gut, including Lactobacillus and Lachnospira, and reduced intestinal inflammation. However, in the mice after gut sterilization by long-term oral antibiotics, QNP@HMs showed no therapeutic effect. QNP@HMs are a promising medication for the prevention of high-altitude sleep disturbance based on the gut-brain axis.

Effect of electrospun poly (L-lactide-co-caprolactone) and formulated porcine fibrinogen for diabetic foot ulcers.

Diabetic foot ulcers were a significant complication of diabetes and were accompanied by delayed wound healing. To compare the effect of topical application electrospun poly (L-lactide-co-caprolactone) and formulated porcine fibrinogen (PLCL/Fg) dressing with alginate dressing when treating diabetic foot ulcers (DFUs). A single-center, prospective, randomized, patient-blinded clinical trial was conducted from July 1, 2023, to December 26, 2023. The clinical trial registration was completed on August 28, 2023 (ClinicalTrials.gov Identifier: NCT06014437). The eligible patients with DFUs of 1-20 cm2 present for at least 1 month and with Wagner grade 1 or 2. They were randomized 1:1 to receive PLCL/Fg or alginate dressing. Participants received PLCL/Fg dressing 1-3 times per week or alginate dressing 3 times per week for 12 weeks. A total of 52 patients (33 men [63.5 %]; mean [SD] age, 63.1 [11.9] years; mean [SD] diabetes time, 8.3 [4.6] years) with DFUs were assessed for this study. The DFUs classified as Wagner grade 1 or 2 (mean [SD] ulcer area, 3.8 [3.2] cm2) were randomized to receive either the PLCL/Fg dressing (n = 26) or the alginate dressing (n = 26) for as long as 12 weeks. In this study, the incidence of complete healing included 22 patients (91.7 %) in the PLCL/Fg group and 14 (63.6 %) in the alginate group during the 12-week treatment period (P = 0.003). The treatment-related adverse events that occurred were 5 (20.8 %) in the PLCL/Fg group and 4 (18.1 %) in the comparator group. In this randomized clinical trial, PLCL/Fg dressing showed beneficial effects in DFUs treatment of wound surface reduction and regulating the wound microenvironment.

Oral alginate microspheres for the efficient site-specific delivery of epidermal growth factor attenuated murine ulcerative colitis via repairing the mucosal barrier.

Ulcerative colitis (UC) is a chronic bowel inflammatory disease affecting the colorectum. Epidermal growth factor (EGF) has been demonstrated to be effective to counteract UC. However, there exists the gastrointestinal challenges such as stomach acid, enzyme and bile salts for oral delivery of EGF. Herein, calcium alginate microsphere was prepared by the microfluidic technique to encapsulate EGF. The morphology of EGF-loaded microsphere (MS-EGF) was spherical and its average particle size was 80 ± 23 µm. The encapsulation efficiency of EGF was reaching to 93.8 % ± 1.6 %. In vitro release experiments showed that MS-EGF presented the good pH-sensitive properties, that was, it could effectively resist the gastric acid and small intestinal fluids, and undergone the rapid dissolution in the artificial colon fluid. In vitro cellular experiments demonstrated that the bioactivity of EGF was well preserved by microsphere. Moreover, in vivo murine colitis model showed that MS-EGF presented the obvious colitis alleviation. Furthermore, the colonic morphology of colitis mice was effectively recovered and the tight junction between the gut epithelium was obviously repaired. In conclusion, calcium alginate microsphere might be a promising vehicle of EGF for UC treatment.

Development of polysaccharide-coated layered double hydroxide nanocomposites for enhanced oral insulin delivery.

Oral insulin (INS) is predicted to have the most therapeutic advantages in treating diabetes to repress hepatic glucose production through its potential to mimic the endogenous insulin pathway. Many oral insulin delivery systems have been investigated. Layered double hydroxide (LDH) as an inorganic material has been widely used in drug delivery thanks to its appealing features such as good biocompatibility, low toxicity, and excellent loading capability. However, when used in oral drug delivery, the effectiveness of LDH is limited due to the acidic degradation in the stomach. In this study, to overcome these challenges, chitosan (Chi) and alginate (Alg) dual-coated LDH nanocomposites with the loading of insulin (Alg-Chi-LDH@INS) were developed by the layered-by-layered method for oral insulin delivery with dynamic size of ~ 350.8 nm, negative charge of ~ - 13.0 mV, and dispersity index 0.228. The insulin release profile was evaluated by ultraviolet-visible spectroscopy. The drug release profiles evidenced that alginate and chitosan coating partially protect insulin release from a burst release in acidic conditions. The analysis using flow cytometry showed that chitosan coating significantly enhanced the uptake of LDH@INS by Caco-2 cells compared to unmodified LDH and free insulin. Further in the in vivo study in streptozocin-induced diabetic mice, a significant hypoglycemic effect was maintained following oral administration with great biocompatibility (~ 50% blood glucose level reduction at 4 h). This research has thus provided a potential nanocomposite system for oral delivery of insulin.

Quaternized oxidized sodium alginate injectable hydrogel with high antimicrobial and hemostatic efficacy promotes diabetic wound healing.

In this paper, a hydrogel material with efficient antibacterial, hemostatic, self-healing, and injectable properties was designed for the treatment of diabetic wounds. Firstly, quaternary ammonium salts were grafted with oxidized sodium alginate, and quaternized oxidized sodium alginate (QOSA) was synthesized. Due to the introduction of quaternary ammonium group it has antibacterial and hemostatic effects, at the same time, due to the presence of aldehyde group it can be reacted with carboxymethyl chitosan (CMCS) to form a hydrogel through the Schiff base reaction. Furthermore, deer antler blood polypeptide (DABP) was loaded into the hydrogel (QOSA&CMCS&DABP), showing good swelling ratio and bacteriostatic effect. In vitro and in vivo experiments demonstrated that the hydrogel not only quickly inhibited hepatic hemorrhage in mice and reduced coagulation index and clotting time in vitro, but also significantly enhanced collagen deposition at the wound site, accelerating wound healing. This demonstrates that the multifunctional hydrogel materials (QOSA&CMCS&DABP) have promising applications in the acceleration of skin wound healing and antibacterial promotion.

STING agonist-based hydrogel enhances immune activation in synergy with radiofrequency ablation for hepatocellular carcinoma treatment.

Immunosuppression caused by incomplete radiofrequency ablation (iRFA) is a crucial factor affecting the effectiveness of RFA for solid tumors. However, little is known about the changes iRFA induces in the tumor immune microenvironment (TIME) of hepatocellular carcinoma (HCC), the primary application area for RFA. In this study, we found iRFA promotes a suppressive TIME in residual HCC tumors, characterized by M2 macrophage polarization, inhibited antigen presentation by dendritic cells (DCs), and reduced infiltration of cytotoxic T lymphocytes (CTLs). Interestingly, the STING agonist MSA-2 was able to reorganize M2-like tumor-promoting macrophages into M1-like anti-tumor states and enhance antigen presentation by DCs. To optimize the therapeutic effect of MSA-2, we used a calcium ion (Ca2+) responsive sodium alginate (ALG) as a carrier, forming an injectable hydrogel named ALG@MSA-2. This hydrogel can change from liquid to gel, maintaining continuous drug release in situ. Our results suggested that ALG@MSA-2 effectively activated anti-tumor immunity, as manifested by increased M1-like macrophage polarization, enhanced antigen presentation by DCs, increased CTL infiltration, and inhibited residual tumor growth. ALG@MSA-2 also resulted in a complete regression of contralateral tumors and widespread liver metastases in vivo. In addition, the excellent biosafety of ALG@MSA-2 was also proved by blood biochemical analysis and body weight changes in mice. In summary, this study demonstrated that the immune cascade of ALG@MSA-2 mediated the STING pathway activation and promoted a favorable TIME which might provide novel insights for the RFA treatment of HCC.

Oral administration of encapsulated catechin in chitosan-alginate nanoparticles improves cognitive function and neurodegeneration in an aluminum chloride-induced rat model of Alzheimer's disease.

The present study aimed to investigate the effect of catechin-loaded Chitosan-Alginate nanoparticles (NPs) on cognitive function in an aluminum chloride (AlCl3)-induced rat model of Alzheimer's disease (AD). The Catechin-loaded Chitosan-Alginate nanocarriers were synthesized through ionotropic gelation (IG) method. Physio-chemical characterization was conducted with the Zetasizer Nano system, the scanning electron microscope, and the Fourier transform infrared spectroscopy. The experiments were performed over 21 days on six groups of male Wistar rats. The control group, AlCl3 treated group, Catechin group, nanocarrier group, treatment group 1 (AlCl3 + Catechin), and treatment group 2 (AlCl3 + nanocarrier). A behavioral study was done by the Morris water maze (MWM) test. In addition, the level of oxidative indices and acetylcholine esterase (AChE) activity was determined by standard procedures at the end of the study. AlCl3 induced a significant increase in AChE activity, along with a significant decrease in the level of Catalase (CAT) and total antioxidant capacity (TAC) in the hippocampus. Moreover, the significant effect of AlCl3 was observed on the behavioral parameters of the MWM test. Both forms of Catechin markedly improved AChE activity, oxidative biomarkers, spatial memory, and learning. The present study indicated that the administration of Catechin-loaded Chitosan-Alginate NPs is a beneficial therapeutic option against behavioral and chemical alteration of AD in male Wistar rats.

Enhancement of cellular activity in hyperglycemic mice dermal wounds dressed with chitosan-alginate membranes

The use of specially designed wound dressings could be an important alternative to facilitate the healing process of wounds in the hyperglycemic state. Biocompatible dressings combining chitosan and alginate can speed up wound healing by modulating the inflammatory phase, stimulating fibroblast proliferation, and aiding in remodeling phases. However, this biomaterial has not yet been explored in chronic and acute lesions of diabetic patients. The aim of this study was to evaluate the effect of topical treatment with a chitosan-alginate membrane on acute skin wounds of hyperglycemic mice. Diabetes mellitus was induced by streptozotocin (60 mg · kg-1 · day-1 for 5 days, intraperitoneally) and the cutaneous wound was performed by removing the epidermis using a surgical punch. The results showed that after 10 days of treatment the chitosan and alginate membrane (CAM) group exhibited better organization of collagen fibers. High concentrations of interleukin (IL)-1α, IL-1β, granulocyte colony-stimulating factor (G-CSF), and tumor necrosis factor-alpha (TNF-α) were detected in the first and second days of treatment. G-CSF and TNF-α level decreased after 5 days, as well as the concentrations of TNF-α and IL-10 compared with the control group (CG). In this study, the inflammatory phase of cutaneous lesions of hyperglycemic mice was modulated by the use of CAM, mostly regarding the cytokines IL-1α, IL-1β, TNF-α, G-CSF, and IL-10, resulting in better collagen III deposition. However, further studies are needed to better understand the healing stages associated with CAM use.

Influence of the geometry of nanostructured hydroxyapatite and alginate composites in the initial phase of bone repair

Abstract Purpose: To analyze, histomorphologically, the influence of the geometry of nanostructured hydroxyapatite and alginate (HAn/Alg) composites in the initial phase of the bone repair. Methods: Fifteen rats were distributed to three groups: MiHA - bone defect filled with HAn/Alg microspheres; GrHA - bone defect filled with HAn/Alg granules; and DV - empty bone defect; evaluated after 15 days postoperatively. The experimental surgical model was the critical bone defect, ≅8.5 mm, in rat calvaria. After euthanasia the specimens were embedded in paraffin and stained with hematoxylin and eosin, picrosirius and Masson-Goldner's trichrome. Results: The histomorphologic analysis showed, in the MiHA, deposition of osteoid matrix within some microspheres and circumjacent to the others, near the bone edges. In GrHA, the deposition of this matrix was scarce inside and adjacent to the granules. In these two groups, chronic granulomatous inflammation was noted, more evident in GrHA. In the DV, it was observed bone neoformation restricted to the bone edges and formation of connective tissue with reduced thickness in relation to the bone edges, throughout the defect. Conclusion: The geometry of the biomaterials was determinant in the tissue response, since the microspheres showed more favorable to the bone regeneration in relation to the granules.

Revestimento edível usando alginato de sódio incorporado com óleo essencial de cravo e extrato de própolis verde na conservação de Mytella guyanensis / Edible coating using clove essential oil and green propolis extract incorporated sodium alginate on the conservation of Mytella guyanensis

Este trabalho teve como objetivo avaliar a atividade antimicrobiana do revestimento edível a base de extrato de própolis verde e óleo de cravo em amostras de sururu (Mytella guyanensis). Foram elaboradas três soluções de revestimento: óleo essencial de cravo a 2 mg.m-1(OEC); própolis verde a 2% (PV2%) e própolis verde a 2% + OEC a 2 mg. mL-1(PV2%+OEC). Sururu sem revestimento foi usado como controle. As amostras foram embaladas e armazenadas a 5°C por 12 dias e realizadas análises microbiológicas (bactérias aeróbias mesófilas, coliformes a 35°C e a 45°C) e físico-químicas (bases voláteis totais e pH) em intervalos de 0, 4, 8 e 12 dias. Os revestimentos edíveis apresentaram baixa redução na carga microbiana de mesófilos, porém com redução significativa (p PV2%+OEC > OEC). A aplicação de revestimento edível a base de PV2% e PV2%+OEC é uma alternativa eficiente para a redução da carga microbiana de coliformes em amostras de sururu refrigerado.

Applicability of knowledge of graduates in dentistry: use of irreversible hydrocolloid / Aplicabilidad del conocimiento de licenciados en odontología: el uso de hidrocoloide irreversible

The objective of this study was to evaluate the knowledge applied by dental students on the procedures of disinfection, tempering and pouring of irreversible hydrocolloid impressions. This study was conducted through a questionnaire to 86 undergraduate students, of both genders, of the eighth and ninth period of the School of Dentistry, Pontifical Catholic University, Belo Horizonte, MG. The questionnaire contained 12 multiple choice questions about clinical and laboratory procedures for dental impression. Analyzed data were descriptively and qualitatively. Most subjects (70%) stated they did disinfection of dental impression with 1% sodium hypochlorite spray. However, they did it in open containers (75.4%) and with time control (68.6%). The ratio water / powder is randomly conducted by most students (60.5%), and tap water is the one most commonly used (95.3%). The mixing of the calcium sulfate is done manually by nearly all students (97.7%), and use vibrator during the pouring of the calcium sulfate is common among undergraduates (60.5%). The setting of the calcium sulfate takes place predominantly exposed to air (93%) and the removal of the model is made ??between 30 and 60 min after pouring by 84.9% of students. These results point to the need for awareness of students of adopting practices transmitted during the undergraduate degree. It is also necessary to investigate the possible causes of knowledge transmission problems and how to effectively adopt good clinical practices.

El objetivo fue evaluar el conocimiento aplicado por estudiantes de odontología en los procedimientos de desinfección, templado y vaciado de las impresiones de hidrocoloides irreversibles. Este estudio se llevó a cabo a través de un cuestionario a 86 estudiantes de pregrado, de ambos sexos, del octavo y noveno período de la Escuela de Odontología de la Pontificia Universidad Católica, Belo Horizonte, MG. El cuestionario contenía 12 preguntas de opción múltiple acerca de los procedimientos clínicos y de laboratorio para impresión dental. Los datos fueron analizados de manera descriptiva y cualitativa. La mayoría de los sujetos (70%) declararon que hicieron desinfección de la impresión dental con pulverización de hipoclorito de sodio al 1%. Sin embargo, lo hicieron en recipientes abiertos (75,4%) y con un control de tiempo (68,6%). La relación agua/polvo se realizó de manera aleatoria por la mayoría de los estudiantes (60,5%), utilizando principalmente agua del grifo (95,3%). La mezcla del sulfato de calcio se realiza manualmente por casi todos los estudiantes (97,7%) y el uso del vibrador durante el vertido del sulfato de calcio es común (60,5%). El ajuste del sulfato de calcio tiene lugar predominantemente en exposición al aire (93%), la remoción del modelo se hace entre 30 y 60 min después del vaciado por un 84,9% de los estudiantes. Estos resultados apuntan a la necesidad de que los estudiantes tomen conciencia de las prácticas transmitidas durante la licenciatura. También es necesario investigar las posibles causas de los problemas de transmisión de conocimiento y cómo aplicar efectivamente las buenas prácticas clínicas.

Ensaio clínico para tratamento da área doadora de enxerto de pele de espessura parcial: aplicação do curativo de colágeno associado ao filme de poliuretano / Clinical trial to treatment of partial-thickness skin graft donor site: application of collagen dressing associated to polyurethane film

Introdução: O tratamento local da área doadora de enxerto de pele é assunto controverso na literatura médica. Revendo esta problemática, procuramos analisar a capacidade do curativo em resolver a ferida e reduzir desconforto e dor dos pacientes. Método: Foram selecionados 34 pacientes que, de forma aleatória e randomizada, compuseram três grupos: grupo controle (GE-C), curativo de rayon embebido em soro fisiológico 0,9%; grupo estudo-A (GEA), curativo de colágeno bovino associado ao alginato de cálcio e coberto por filme de poliuretano transparente; grupo estudo-B (GE-B), somente filme de poliuretano transparente. Dois observadores independentes avaliaram os pacientes dos três grupos em relação ao desempenho de cada curativo: epitelização, formação de crosta, quantidade e características do exsudato e presença de complicações. A dor foi avaliada por meio da escala visual analógica (EVA), pelo inventário breve de dor (IBD) e índice de manejo da dor (IMD). Resultados: Ao compararmos o GEA em relação ao tempo de epitelização com os outros grupos encontramos, GE-A: 6,3 dias/GE-B: 8,2 dias com p < 0,02; GEA:6,3 dias/GE-C: 11,7 dias com p < 0,01. Em 90% dos pacientes do GEA, registramos dor leve (EVA), 85% do GEA referiu dor não localizada na área doadora de enxerto (IBD) e o IMD ficou entre o intervalo [-3;+1]. Conclusão: Os autores sugerem que a utilização do curativo de colágeno associado ao alginato de cálcio em áreas doadoras de enxerto poderá reduzir o tempo para total epitelização e queixas álgicas e promover maior conforto ao paciente.

Background: Local treatment of the donor site skin graft is controversial in medical literature. Reviewing that issue, we have analyzed the capacity of the dressing random and randomly in solving the wound and reduce both discomfort and pain of patients. Methods: We have random selected 34 patients and randomly divided into three groups: control group (GEC), rayon dressing soaked in saline 0.9% - study group A (GE-A), bovine collagen dressing associated with the alginate calcium and covered with transparent polyurethane film - study group B (GE-B), only transparent polyurethane film. Two independent observers assayed the patients in the three groups regarding the performance of each dressing epithelialization, scabbing, quantity and characteristics of the exudate and the presence of complications. Pain was quantified by: visual analog scale (VAS), the brief pain inventory (IBD) and index of pain management (IMD). Results: Comparing the GEA with respect to time of epithelialization with other groups found, GE-A: 6.3 days / GE-B: 8.2 days p <0.02; GE-A: 6.3 days / GE-C: 11.7 days with p <0.01. In 90% of patients in the GEA recorded mild pain (VAS), 85% of the GEA did not mention pain localized in the donor graft (IBD) and the IMD was between the interval [-3; +1]. Conclusion: The authors suggest that the use of the dressing of collagen associated with calcium alginate in areas of donor grafts may reduce the time for complete epithelization and pain complaints and promote greater patient comfort.

Evaluación del gel antiadherencial post quirúrgico: hialuronato de sodio más carboximetilcelulosa y alginato / Evaluation of post-surgical antiadherence gel: sodium hyaluronate plus carboxymethylcellulose and alginate

INTRODUCCIÓN: Antecedentes: Las adherencias son bandas de tejido similar el tejido cicatricial que se forman entre superfícies dentro del organismo y hacen que estas se unan. Se forman más frecuentemente en el abdomen y pelvis después de cirugias intestinales o de los órganos femeninos, estas producen diversar molestias como distención o hinchazón abdominal, estreñimiento, náuseas y vomitos, incluso dolor pélvico intenso y crónico. Las adherencias son parte de un proceso fisiopatológico desencadenado o en respuesta al corte, isquemia, abrasión del peritoneo durante la cirugia. Las zonas alteradas sufren de regeneración mesotelial y fibrosis resultando en la formación de adherencias entre los tejidos serosos dañados. Las adherencias pueden tener un efecto beneficioso, ya que permite neovscularización de zonas isquémicas del intestino, tales como en las anastomosis, pero generalmente ocasionan probelmas cuando se vuelven excesivas. Las adherencias son la principal causa de obstrucción intestinal que causa el 70% de readmisiones hospitalarias. El hialuronato de sodio más carboximetilcelulosa, es descrito como un agente antiadherente no tóxico, no inmunogénico y biocompatible que reduce la incidencia y extensión de las adherencias postoperatorias. Compuesto de hialuronato de sodio y carboximetilcelulosa está modificado químicamente para retardar su tasa de degradación y deputación después de entrar en contato con el organismo. METODOLOGÍA: Se realizó una búsqueda bibliográfica de la literatura con respecto a la eficacia y seguridad del uso del Gel Antiadherencial Post quirúrgico. Se seleccionaron artículos científicos: evaluación de tecnologías santiarias, revisiones sistemáticas, met-análisis y ensayos clínicos. Se realizó una búsqueda dentro de la información generada por grupos que realizan revisiones sistemáticas, evaluación de tencologías sanitarias y guías de práctica clínica, tales como The Cochrane Library y The National Institute for Health and Care Excelence (NICE). Además, se hizo una búsqueda en www.clinicaltrials.gov, para poder identificar ensayos aún en elaboración o que no hayan sido publicados. CONCLUSIONES: Las investigaciones publicadas hasta marzo del 2016 son insuficientes para poder determinar la eficacia y seguridad del gel antiadherencial postquirúrgico (hialuronato de sodio más carboximetilcelulosa). Los resultados de los estudios preclínicos son discordantes con los ensayos clínicos realizados posteriomente. El Instituto de Tecnologías en Salud e Investigación-IETSI, no aprueba el uso del gel antiadherencial en sus presentaciones: (hialuronato de sodio más carboximetilcelulosa, alginato o la combinación de hialuronato de sodio más carboximetilcelulosa y alginato; para la reducción de adherencias abdominlaes y pélvicas.

Desinfecção, acondicionamento e vazamento de moldes de alginato por alunos de graduação / Desinfection, tempering and pouring of irreversiblie hydrocolloid impressions by dental undergraduates

Devido à alta aplicabilidade do alginato e do gesso pedra na odontologia, o presente estudo teve como proposição avaliaros procedimentos de desinfecção, acondicionamento evazamento de moldes de alginato por acadêmicos na clínica odontológica da UNESP/CSJCAMPOS, bem como sugerirmanobras a fim de otimizarem os resultados desses trabalhosna clínica diária. Para tanto, um questionário com perguntas abertas e fechadas foi aplicado a 63 alunos do último ano docurso de odontologia. Baseado nos resultados, os seguintes procedimentos foram sugeridos: providenciar água destilada para a manipulação do gesso; alertar os acadêmicos sobre a importância de uma espatulação vigorosa do gesso por no máximo 1 minuto, além de conscientizá - los da necessidade demanter o molde sempre no umidificador depois de ter sidovertido com gesso