RESUMO
A rapid, highly sensitive, and selective method was applied in a non-invasive way to investigate the antidepressant action of Xiaoyaosan (XYS) using ultra performance liquid chromatography-mass spectrometry (UPLC-MS) and chemometrics. Many significantly altered metabolites were used to explain the mechanism. Venlafaxine HCl and fluoxetine HCl were used as chemical positive control drugs with a relatively clear mechanism of action to evaluate the efficiency and to predict the mechanism of action of XYS. Urine obtained from rats subjected to chronic unpredictable mild stress (CUMS) was analyzed by UPLC-MS. Distinct changes in the pattern of metabolites in the rat urine after CUMS production and drug intervention were observed using partial least squares-discriminant analysis. The results of behavioral tests and multivariate analysis showed that CUMS was successfully reproduced, and a moderate-dose XYS produced significant therapeutic effects in the rodent model, equivalent to those of the positive control drugs, venlafaxine HCl and fluoxetine HCl. Metabolites with significant changes induced by CUMS were identified, and 17 biomarker candidates for stress and drug intervention were identified. The therapeutic effect of XYS on depression may involve regulation of the dysfunctions of energy metabolism, amino acid metabolism, and gut microflora changes. Metabonomic methods are valuable tools for measuring efficacy and mechanisms of action in the study of traditional Chinese medicines.
Assuntos
Antidepressivos/uso terapêutico , Medicamentos de Ervas Chinesas/uso terapêutico , Trato Gastrointestinal/microbiologia , Redes e Vias Metabólicas/efeitos dos fármacos , Microbiota/efeitos dos fármacos , Fitoterapia , Animais , Antidepressivos/urina , Benzoatos/urina , Biomarcadores/urina , Hidrocarbonetos Aromáticos com Pontes/urina , Catequina/urina , Chalcona/análogos & derivados , Chalcona/urina , Cromatografia Líquida , Ácido Cítrico/urina , Ciclo do Ácido Cítrico/efeitos dos fármacos , Ácidos Cumáricos/urina , Creatina Quinase/efeitos dos fármacos , Creatina Quinase/urina , Creatinina/urina , Cicloexanóis/uso terapêutico , Medicamentos de Ervas Chinesas/análise , Flavanonas/urina , Fluoxetina/uso terapêutico , Ácido Gálico/urina , Glucosídeos/urina , Glicina/análogos & derivados , Glicina/efeitos dos fármacos , Glicina/urina , Hipuratos/urina , Ácidos Cetoglutáricos/urina , Ácido Cinurênico/urina , Masculino , Espectrometria de Massas , Metabolômica , Monoterpenos , Extratos Vegetais/uso terapêutico , Ratos , Ratos Sprague-Dawley , Estresse Psicológico/tratamento farmacológico , Triptofano/efeitos dos fármacos , Triptofano/urina , Tirosina/efeitos dos fármacos , Tirosina/urina , Cloridrato de VenlafaxinaRESUMO
As the most important marker component in Carthamus tinctorius L., hydroxysafflor yellow A (HSYA) was widely used in the prevention and treatment of cardiovascular diseases, due to its effect of improving blood supply, suppressing oxidative stress, and protecting against ischemia/reperfusion. In this paper, both an in vitro microsomal incubation and an in vivo animal experiment were conducted, along with an LC-Q-TOF/MS instrument and a 3-step protocol, to further explore the metabolism of HSYA. As a result, a total of 10 metabolites were searched and tentatively identified in plasma, urine, and feces after intravenous administration of HSYA to male rats, although no obvious biotransformation was found in the simulated rat liver microsomal system. The metabolites detected involving both phase I and phase II metabolism including dehydration, deglycosylation, methylation, and glucuronic acid conjugation. A few of the metabolites underwent more than one-step metabolic reactions, and some have not been reported before. The study would contribute to a further understanding of the metabolism of HSYA and provide scientific evidence for its pharmacodynamic mechanism research and clinical use.
Assuntos
Chalcona/análogos & derivados , Quinonas/metabolismo , Animais , Chalcona/sangue , Chalcona/metabolismo , Chalcona/urina , Cromatografia Líquida de Alta Pressão/métodos , Desidratação , Ácido Glucurônico/metabolismo , Masculino , Metilação , Microssomos Hepáticos/metabolismo , Quinonas/sangue , Quinonas/urina , Ratos Sprague-Dawley , Espectrometria de Massas por Ionização por Electrospray/métodosRESUMO
A rapid and specific high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the quantification of hydroxysafflor yellow A (HSYA) in human urine with isorhamnetin-3-O-neohespeidoside as internal standard (IS). HSYA and IS were extracted from urine samples by simple solid-phase extraction and separated on an Agilent Zorbax SB C18 column (4.6 mm × 150 mm, 5 µm) with the mobile phase of 0.2 mM ammonium acetate: methanol (30/70, v/v) at a flow rate of 0.4 mL/min. Polar endogenous interferences eluted in 0.1-2.5 min were switched into waste channel by the Valve Valco, to reduce the possible matrix effect for MS detection in each run. The MS detection of analytes was performed on a tandem mass spectrometer equipped with an electrospray ionization source in negative mode using multiple-reaction monitoring. The MS/MS ion transitions monitored were m/z 611.3â491.2 for HSYA and m/z 623.2â299.2 for IS. The method was fully validated for selectivity, sensitivity, linearity, precision, accuracy, recovery, matrix effect and stability, and then was applied to the urinary excretion study of injectable powder of pure HSYA in healthy Chinese volunteers for the first time. The results suggested that urine was the main excretion way of HSYA in healthy volunteers, further demonstrating the feasibility and necessity of our current method.
Assuntos
Chalcona/análogos & derivados , Cromatografia Líquida de Alta Pressão/métodos , Quinonas/urina , Espectrometria de Massas em Tandem/métodos , Adulto , Chalcona/isolamento & purificação , Chalcona/urina , Humanos , Masculino , Quinonas/isolamento & purificação , Sensibilidade e Especificidade , Extração em Fase Sólida , Adulto JovemRESUMO
The major proportion of oral doses of 14C-mecinarone was excreted in the faeces by rat, dog and man, and in all species the faecal metabolites were more polar than mecinarone and the O-desmethyl reference compounds. Rat faecal extracts contained two major components each accounting for about 30-40% of the radioactivity. Dog and human faecal extracts contained some mecinarone but also three major, more polar components, two of which corresponded to the rat metabolites. Rat bile contained three major components and dog bile two components. One of the components in both bile samples was shown to be a conjugate of O-desmethyl-mecinarone. Besides mecinarone human urine contained a component corresponding to the phenol resulting from 0-demethylation in the p-methoxycinnamoyl group. The same two compounds were also detected in human plasma. The two major components in rat and dog faecal extracts gave mass spectra identical to mecinarone and the p-hydroxycinnamoyl derivative (O-desmethyl-mecinarone). It is postulated that these thermally-labile metabolites were formed by nucleophilic addition of a substituent to the alpha, beta-unsaturated ketone. It has been demonstrated in vitro that mecinarone forms a glutathione conjugate. The metabolites may be compounds of this type where the glutathione moiety has been degraded in the gastrointestinal tract.
Assuntos
Benzofuranos/metabolismo , Vasodilatadores/metabolismo , Animais , Benzofuranos/sangue , Benzofuranos/urina , Bile/metabolismo , Biotransformação , Chalcona/análogos & derivados , Chalcona/sangue , Chalcona/metabolismo , Chalcona/urina , Cães , Fezes/análise , Feminino , Glutationa/metabolismo , Humanos , Técnicas In Vitro , Fígado/metabolismo , Masculino , Espectrometria de Massas , Ratos , Especificidade da Espécie , Vasodilatadores/sangue , Vasodilatadores/urinaRESUMO
Studies were conducted to characterize the pharmacokinetics and excretion of hydroxysafflor yellow A (HSYA) in rats and dogs after administration by intravenous injection or infusion. Plasma, urine, feces and bile concentrations of HSYA were measured using five validated mild HPLC methods. Linear pharmacokinetics of HSYA after the intravenous administrations were found at doses ranging from 3 to 24 mg/kg in rats and from 6 to 24 mg/kg in dogs. At a dose of 3 mg/kg, HSYA in urine, feces and bile was determined. For 48 h after dosing, the amount of urinary excretion accounted for 52.6 +/- 17.9 % (range: 31.1 - 78.7%, n = 6) of the dose, and the amount of fecal amount accounted for 8.4 +/- 5.3% (range 1.7 - 16.4%, n = 6) of the dose. Biliary excretion amount accounted for 1.4 +/- 1.0% (range 0.4-2.9%; n = 6) of the dose for 24 h after dosing. Percent plasma protein binding of HSYA ranged from 48.0 to 54.6% at 72 h. In summary, five mild HPLC methods for the determinations of HSYA in rat plasma, urine, feces, bile and dog plasma have been developed and successfully applied to preclinical pharmacokinetics and excretion of HSYA in rats and dogs. The results of excretion studies indicated that HSYA was rapidly excreted as unchanged drug in the urine. In view of previous pharmacological work, the concentration-dependent neuroprotective effect of HSYA in rats was defined.
Assuntos
Carthamus tinctorius , Chalcona/análogos & derivados , Fármacos Neuroprotetores/farmacocinética , Fitoterapia , Pigmentos Biológicos/farmacocinética , Quinonas/farmacocinética , Animais , Área Sob a Curva , Bile/metabolismo , Chalcona/administração & dosagem , Chalcona/sangue , Chalcona/química , Chalcona/farmacocinética , Chalcona/urina , Cães , Fezes/química , Infusões Intravenosas , Injeções Intravenosas , Fármacos Neuroprotetores/administração & dosagem , Fármacos Neuroprotetores/sangue , Fármacos Neuroprotetores/química , Fármacos Neuroprotetores/urina , Pigmentos Biológicos/administração & dosagem , Pigmentos Biológicos/sangue , Pigmentos Biológicos/química , Pigmentos Biológicos/urina , Extratos Vegetais/administração & dosagem , Extratos Vegetais/sangue , Extratos Vegetais/química , Extratos Vegetais/farmacocinética , Extratos Vegetais/urina , Ligação Proteica/efeitos dos fármacos , Quinonas/administração & dosagem , Quinonas/sangue , Quinonas/química , Quinonas/urina , Ratos , Ratos Sprague-DawleyRESUMO
The metabolism of 2',4',4-, 2',6',3- and 2',6',4-trihydroxydihydrochalcone was studied in rats. Approx. 25-35% of the oral dose (0.75 mmol/kg) was excreted in the urine during a five to six day period. The unchanged compounds were the most prominent urinary excretion products and were also present in large amounts in the faeces. Other metabolites of the three dihydrochalcones included minor amounts of hydroxylated products and subsequent O-methylated derivatives as well as minor amounts of degradation products (resorcinol and hydroxyphenylpropionic acids) arising from scission of the compounds by the normal gut microflora. The present results support the view that the degradative metabolism of dihydrochalcones involves scission leading to equal amounts of products derived from the A- and B-ring regions of these flavonoids.
Assuntos
Chalcona/análogos & derivados , Chalcona/urina , Propiofenonas/urina , Animais , Chalconas , Fenômenos Químicos , Química , Hidroxilação , Masculino , Espectrometria de Massas , Metilação , Floretina , Ratos , Ratos EndogâmicosRESUMO
A high-performance liquid chromatographic method has been developed for the analysis of the novel antiparasitic agent, licochalcone A (Lica), and three of its glucuronic acid conjugates in plasma and urine. The high-performance liquid chromatography assay was performed using gradient elution and UV detection at 360 nm. The proposed technique is selective, reliable and sensitive. The limits of quantification for Lica are 0.2 microg/ml in plasma and 0.14 microg/ml in urine, 1.2 microg/ml for the 4'-glucuronide in plasma and 1.4 microg/ml in urine, and 2.0 microg/ml for the 4-glucuronide in plasma and 3.2 microg/ml in urine. The reproducibility of the analytical method according to the statistical coefficients is 7% or below. The accuracy of the method is good, that is, the relative error is below 10%. The stability of Lica and its glucuronides in urine and plasma samples has been assessed during storage in the autosampler and freezer. The applicability of the assay for determining Lica and its intact glucuronide conjugates in biological fluids was shown using a single dose study in rat.