RESUMO
Recently, the IUCr (International Union of Crystallography) initiated the formation of a Diffraction Data Deposition Working Group with the aim of developing standards for the representation of raw diffraction data associated with the publication of structural papers. Archiving of raw data serves several goals: to improve the record of science, to verify the reproducibility and to allow detailed checks of scientific data, safeguarding against fraud and to allow reanalysis with future improved techniques. A means of studying this issue is to submit exemplar publications with associated raw data and metadata. In a recent study of the binding of cisplatin and carboplatin to histidine in lysozyme crystals under several conditions, the possible effects of the equipment and X-ray diffraction data-processing software on the occupancies and B factors of the bound Pt compounds were compared. Initially, 35.3â GB of data were transferred from Manchester to Utrecht to be processed with EVAL. A detailed description and discussion of the availability of metadata was published in a paper that was linked to a local raw data archive at Utrecht University and also mirrored at the TARDIS raw diffraction data archive in Australia. By making these raw diffraction data sets available with the article, it is possible for the diffraction community to make their own evaluation. This led to one of the authors of XDS (K. Diederichs) to re-integrate the data from crystals that supposedly solely contained bound carboplatin, resulting in the analysis of partially occupied chlorine anomalous electron densities near the Pt-binding sites and the use of several criteria to more carefully assess the diffraction resolution limit. General arguments for archiving raw data, the possibilities of doing so and the requirement of resources are discussed. The problems associated with a partially unknown experimental setup, which preferably should be available as metadata, is discussed. Current thoughts on data compression are summarized, which could be a solution especially for pixel-device data sets with fine slicing that may otherwise present an unmanageable amount of data.
Assuntos
Cristalografia por Raios X , Curadoria de Dados/métodos , Austrália , Curadoria de Dados/economia , Bases de Dados de Compostos Químicos , Processamento de Imagem Assistida por Computador , Sociedades Científicas , Software , Difração de Raios XRESUMO
NIH administrators have recently expressed concerns about the cost of curation for biological databases. However, they did not articulate the exact costs of curation. Here we calculate the cost of biocuration of articles for the EcoCyc database as $219 per article over a 5-year period. That cost is 6-15% of the cost of open-access publication fees for publishing biomedical articles, and we estimate that cost is 0.088% of the cost of the overall research project that generated the experimental results. Thus, curation costs are small in an absolute sense, and represent a miniscule fraction of the cost of the research.
Assuntos
Curadoria de Dados/economia , Bases de Dados Factuais/economia , Custos e Análise de Custo , National Institutes of Health (U.S.) , Estados UnidosRESUMO
Can we decrease the costs of database curation by crowd-sourcing curation work or by offloading curation to publication authors? This perspective considers the significant experience accumulated by the bioinformatics community with these two alternatives to professional curation in the last 20 years; that experience should be carefully considered when formulating new strategies for biological databases. The vast weight of empirical evidence to date suggests that crowd-sourced curation is not a successful model for biological databases. Multiple approaches to crowd-sourced curation have been attempted by multiple groups, and extremely low participation rates by 'the crowd' are the overwhelming outcome. The author-curation model shows more promise for boosting curator efficiency. However, its limitations include that the quality of author-submitted annotations is uncertain, the response rate is low (but significant), and to date author curation has involved relatively simple forms of annotation involving one or a few types of data. Furthermore, shifting curation to authors may simply redistribute costs rather than decreasing costs; author curation may in fact increase costs because of the overhead involved in having every curating author learn what professional curators know: curation conventions, curation software and curation procedures.
Assuntos
Crowdsourcing/métodos , Curadoria de Dados/métodos , Bases de Dados Factuais , Modelos Teóricos , Custos e Análise de Custo , Crowdsourcing/economia , Curadoria de Dados/economia , Humanos , Publicações Periódicas como AssuntoRESUMO
Databases and data repositories provide essential functions for the research community by integrating, curating, archiving and otherwise packaging data to facilitate discovery and reuse. Despite their importance, funding for maintenance of these resources is increasingly hard to obtain. Fueled by a desire to find long term, sustainable solutions to database funding, staff from the Arabidopsis Information Resource (TAIR), founded the nonprofit organization, Phoenix Bioinformatics, using TAIR as a test case for user-based funding. Subscription-based funding has been proposed as an alternative to grant funding but its application has been very limited within the nonprofit sector. Our testing of this model indicates that it is a viable option, at least for some databases, and that it is possible to strike a balance that maximizes access while still incentivizing subscriptions. One year after transitioning to subscription support, TAIR is self-sustaining and Phoenix is poised to expand and support additional resources that wish to incorporate user-based funding strategies. Database URL: www.arabidopsis.org.
Assuntos
Arabidopsis/genética , Curadoria de Dados/economia , Modelos Teóricos , Apoio à Pesquisa como Assunto/economia , Bases de Dados Genéticas , SoftwareRESUMO
The manual curation of the information in biomedical resources is an expensive task. This article argues the value of this approach in comparison with other apparently less costly options, such as automated annotation or text-mining, then discusses ways in which databases can make cost savings by sharing infrastructure and tool development. Sharing curation effort is a model already being adopted by several data resources. Approaches taken by two of these, the Gene Ontology annotation effort and the IntAct molecular interaction database, are reviewed in more detail. These models help to ensure long-term persistence of curated data and minimizes redundant development of resources by multiple disparate groups.
Assuntos
Curadoria de Dados/métodos , Mineração de Dados/métodos , Bases de Dados Genéticas , Ontologia Genética , Curadoria de Dados/economia , Mineração de Dados/economiaRESUMO
Identifying concepts and relationships in biomedical text enables knowledge to be applied in computational analyses. Many biological natural language processing (BioNLP) projects attempt to address this challenge, but the state of the art still leaves much room for improvement. Progress in BioNLP research depends on large, annotated corpora for evaluating information extraction systems and training machine learning models. Traditionally, such corpora are created by small numbers of expert annotators often working over extended periods of time. Recent studies have shown that workers on microtask crowdsourcing platforms such as Amazon's Mechanical Turk (AMT) can, in aggregate, generate high-quality annotations of biomedical text. Here, we investigated the use of the AMT in capturing disease mentions in PubMed abstracts. We used the NCBI Disease corpus as a gold standard for refining and benchmarking our crowdsourcing protocol. After several iterations, we arrived at a protocol that reproduced the annotations of the 593 documents in the 'training set' of this gold standard with an overall F measure of 0.872 (precision 0.862, recall 0.883). The output can also be tuned to optimize for precision (max = 0.984 when recall = 0.269) or recall (max = 0.980 when precision = 0.436). Each document was completed by 15 workers, and their annotations were merged based on a simple voting method. In total 145 workers combined to complete all 593 documents in the span of 9 days at a cost of $.066 per abstract per worker. The quality of the annotations, as judged with the F measure, increases with the number of workers assigned to each task; however minimal performance gains were observed beyond 8 workers per task. These results add further evidence that microtask crowdsourcing can be a valuable tool for generating well-annotated corpora in BioNLP. Data produced for this analysis are available at http://figshare.com/articles/Disease_Mention_Annotation_with_Mechanical_Turk/1126402.
Assuntos
Biologia Computacional/métodos , Crowdsourcing/métodos , PubMed , Indexação e Redação de Resumos , Adulto , Benchmarking , Biologia Computacional/economia , Custos e Análise de Custo , Crowdsourcing/economia , Curadoria de Dados/economia , Curadoria de Dados/métodos , Doença , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Processamento de Linguagem Natural , Aprendizado de Máquina Supervisionado , Unified Medical Language System , Adulto JovemAssuntos
Bancos de Espécimes Biológicos/organização & administração , Pesquisa Biomédica/métodos , Curadoria de Dados , Gestão da Informação em Saúde/métodos , Bancos de Espécimes Biológicos/economia , Bancos de Espécimes Biológicos/normas , Pesquisa Biomédica/economia , Pesquisa Biomédica/organização & administração , Comércio , Curadoria de Dados/economia , Curadoria de Dados/métodos , Curadoria de Dados/normas , Gerenciamento de Dados/economia , Gerenciamento de Dados/métodos , Gerenciamento de Dados/organização & administração , Gerenciamento de Dados/normas , Bases de Dados Factuais/normas , Gestão da Informação em Saúde/economia , Gestão da Informação em Saúde/organização & administração , Gestão da Informação em Saúde/normas , Humanos , Gestão da Informação/economia , Gestão da Informação/métodos , Gestão da Informação/organização & administração , Gestão da Informação/normas , Inovação Organizacional/economia , Fluxo de TrabalhoRESUMO
BACKGROUND: This article describes capture of biological information using a hybrid approach that combines natural language processing to extract biological entities and crowdsourcing with annotators recruited via Amazon Mechanical Turk to judge correctness of candidate biological relations. These techniques were applied to extract gene- mutation relations from biomedical abstracts with the goal of supporting production scale capture of gene-mutation-disease findings as an open source resource for personalized medicine. RESULTS: The hybrid system could be configured to provide good performance for gene-mutation extraction (precision â¼82%; recall â¼70% against an expert-generated gold standard) at a cost of $0.76 per abstract. This demonstrates that crowd labor platforms such as Amazon Mechanical Turk can be used to recruit quality annotators, even in an application requiring subject matter expertise; aggregated Turker judgments for gene-mutation relations exceeded 90% accuracy. Over half of the precision errors were due to mismatches against the gold standard hidden from annotator view (e.g., incorrect EntrezGene identifier or incorrect mutation position extracted), or incomplete task instructions (e.g., the need to exclude nonhuman mutations). CONCLUSIONS: The hybrid curation model provides a readily scalable cost-effective approach to curation, particularly if coupled with expert human review to filter precision errors. We plan to generalize the framework and make it available as open source software. DATABASE URL: http://www.mitre.org/publications/technical-papers/hybrid-curation-of-gene-mutation-relations-combining-automated.