New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA
Rev. Soc. Bras. Med. Trop
; 53: e20190333, 2020. graf
Article
em En
| LILACS
| ID: biblio-1092187
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract INTRODUCTION:
Phylogenetic analysis of the 16S ribosomal gene initial region is used to identify Leptospira isolates at the species level from clinical samples. Unfortunately, this method cannot differentiate between some intermediates and saprophytic species.METHODS:
We used comparative genomic analysis between 35 Leptospira species to find new molecular targets for Leptospira species identification.RESULTS:
We proposed the use of the rpoC gene, encoding the DNA-directed RNA polymerase β-subunit, for identifying 35 Leptospira species.CONCLUSIONS:
The rpoC gene can be a molecular target to identify the main species of the Leptospira genus directly from clinical samples.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
DNA Bacteriano
/
RNA Ribossômico 16S
/
Leptospira
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article