Producción y caracterización de anticuerpos monoclonales dirigidos contra la proteína mayor de membrana externa y contra el lipopolisacárido de Chlamydia trachomatis / [Production and characterization of monoclonal antibodies directed agains the major outer membrane protein and lipopolysaccharide of Chlamydia trachomatis]

ABSTRACT

Two monoclonal antibodies (MnAb) directed against the lipopolysaccharide (LPS) and major outer membrane protein (MOMP) of Chlamydia trachomatis were produced for use in indirect immunofluorescence (IFI). The specificity of the antibodies was determined by Dot-blot, Immunoblotting (IB) and IFI onto culture cells infected with C. trachomatis and IFI onto commercial swabs (MRL). The MnAb 2D3 and 3C2 detected LPS and MOMP of C. trachomatis, respectively, by different methods. Neither MnAb showed cross-reactions when other gram-negative bacteria were used as antigens.