Isoenzyme analysis of Arthrobotrys, a nematode-trapping fungus
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;30(10): 1149-52, Oct. 1997. ilus
Article
em En
| LILACS
| ID: lil-201529
Biblioteca responsável:
BR1.1
RESUMO
Extraction and isoenzyme analysis of four isolates of Arthrobotrys including A. musiformis, A. robusta and A. conoides were conducted. Among the 14 enzymes studied by starch gel electrophoresis, using morpholine-citrate as gel/electrode buffer, the following nine enzymes showed interpretable banding patterns alpha-esterase, fumarase, hexokinase, isocitrate dehydrogenase, leucine aminopeptidase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucomutase and phosphoglucoisomerase. All isolates studied displayed typical isoenzyme phenotypes for each species. Two isolates of A. conoides differed in their alpha-isoesterase banding patterns, but no differences were observed for the other enzymes. The assay was satisfactory for enzyme extraction and resolution of Arthrobotrys and could be used in future taxonomic and genetic studies of this organism.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Técnicas In Vitro
/
Fungos
/
Isoenzimas
/
Nematoides
Limite:
Animals
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article