Optimacion del proceso de production de la vacina pertussis / Optimization of pertussis vaccine production process
Acta cient. venez
; 46(2): 129-34, 1995. tab, graf
Article
em Es
| LILACS
| ID: lil-217138
Biblioteca responsável:
BR1.1
RESUMO
The production of Pertussis Vaccine was reevaluated at the Instituto Nacional de Higiene(r)Rafael Rangel(r) in order to optimise it in terms of vaccine yield, potency, specific toxicity and efficiency (cost per doses). Four different processes, using two culture media (Cohen-Wheeler and Fermentacion Glutamato Prolina-1) and two types of bioreactors (25 L Fermentador Caracas and a 450 L industrial fermentor) were compared. Runs were started from freeze-dried strains (134 or 509) andcontinued until the obtention of the maximal yield. It was found that the combination Fermentacion Glutamato Prolina-1/industrial fermentor, shortened the process to 40 hours while consistently yielding a vaccine of higher potency (7.91 +/- 2.56 IU/human dose) and lower specific toxicity in a mice bioassay. In addition, the physical aspect of the preparation was rather homogeneous and free of dark aggregates. Most importantly, the biomass yield more than doubled those of the Fermentador Caracas using the two different media and that in the industrial fermentor with the Cohen-Wheeler medium. Therefore, the cost per doses was substantially decreased
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Vacina contra Coqueluche
Tipo de estudo:
Health_economic_evaluation
Limite:
Animals
Idioma:
Es
Ano de publicação:
1995
Tipo de documento:
Article