Three
Enterococcus faecalis and one
Enterococcus faecium strains were characterized by
plasmid profile,
pulsed-field gel electrophoresis (PFGE) and
determination of antimicrobial minimal inhibitory concentrations. VanA
elements were characterized by Long
PCR , overlapping
PCR and
DNA sequencing . Enterococcal
strains showed resistance to
vancomycin and harbored the vanA
gene , and three these were
teicoplanin susceptible while one showed intermediate resistance to
teicoplanin . Two E. faecalis
strains showed indistinguishable PFGE profile while the third was unrelated. E. faecalis
strains showed a deletion in the right terminal region of the Tn1546-like
element . The E. faecium
strain showed an insertion
element in the vanXY
intergenic region .
Mutations in VanA
elements were not found. Rearrangements in the VanA
element could be responsible for incongruities in
genotype and
phenotype in these
strains .