Transcription of meiotic-like-pathway genes in Giardia intestinalis
Mem. Inst. Oswaldo Cruz
; 103(4): 347-350, June 2008. graf, tab
Article
em En
| LILACS
| ID: lil-486873
Biblioteca responsável:
BR1.1
ABSTRACT
The reproductive mechanism of Giardia intestinalis, considered one of the earliest divergent eukaryotes, has not been fully defined yet. Some evidence supports the hypothesis that Giardia is an exclusively asexual organism with a clonal population structure. However, the high genetic variability, the variation in ploidy during its life cycle, the low heterozygosity and the existence of genes involved in the meiotic-like recombination pathway in the parasite's genome cast doubt on exclusively asexual nature of Giardia. In this work, semiquantitative RT-PCR analysis was used to assess the transcription pattern of three meiosis-like-specific genes involved in homologues recombination dmc1, hop1 and spo11. The mRNAs were amplified during the parasite's differentiation processes, encystation and excystation, and expression was found at each stage of its life cycle. A semiquantitative assessment also suggests that expression of some of the genes is regulated during encystation process.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Giardia lamblia
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Genes de Protozoários
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Meiose
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article
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Project document