Infection kinetics of human adenovirus serotype 41 in HEK 293 cells
Siqueira-Silva, Joselma; Yeda, Fernanda Perez; Favier, Anne-Laure; Mezin, Paulette; Silva, Misael Leonardo; Barrella, Karina Médici; Mehnert, Dolores Úrsula; Fender, Pascal; Hársi, Charlotte Marianna.
Mem. Inst. Oswaldo Cruz
; 104(5): 736-744, Aug. 2009. ilus
Artigo
em Inglês
| LILACS | ID: lil-528083
The purpose of this work was to acquire an overview of the infectious cycle of HAdV-41 in permissive HEK 293 cells and compare it to that observed with the prototype of the genus, Human adenovirus C HAdV-2. HEK 293 cells were infected with each virus separately and were harvested every 12 h for seven days. Infection kinetics were analysed using confocal and electronic microscopy. The results show that, when properly cultivated, HAdV-41 was not fastidious. It had a longer multiplication cycle, which resulted in the release of complete viral particles and viral stocks reached high titres. After 60 h of infection, the export of viral proteins from the infected cell to the extracellular milieu was observed, with a pattern similar to that previously described for HAdV-2 penton-base trafficking after 30 h of infection. HAdV-41 had a non-lytic cycle and the infection spread from the first infected cell to its neighbours. The release process of the viral particles is unknown. The results observed for HAdV-41 infection in HEK 293 cells show how different this virus is from the prototype HAdV-2 and provides information for the development of this vector for use in gene therapy.
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