Mayor actividad de rho kinasa en leucocitos circulantes se asocia a estrés oxidativo y rigidez arterial en hipertensos diabéticos / Increased Rho-kinase activity in peripheral leukocytes is associated to oxidative stress and decreased compliance of the arterial wall in diabetic hypertensive patients
Rev. chil. cardiol
; 30(1): 34-41, 2011.
Article
em Es
| LILACS
| ID: lil-592039
Biblioteca responsável:
CL1.1
RESUMEN
Introducción:
La vía intracellular de RhoA/Rho kinasa es activada por agonistas de receptores acoplados a proteínas G pequeñas unidas a membrana. Su activación está relacionada al remodelado cardiovascular patológico. Previamente hemos observado aumento de actividad de Rho kinasa (ROCK) en pacientes con hipertensión arterial (HT) e hipertrofia ventricular izquierda como daño de órgano blanco. Pero su activación en relación a la diabetes no ha sido explorada en estos pacientes.Objetivo:
Evaluar activación de Rho kinasa y parámetros de estrés oxidativo en pacientes hipertensos con diabetes tipo II (DMII).Métodos:
Estudio comparativo entre pacientes con HT sin tratamiento, HT con DMII y hemoglobina glicosi-lada Alc > 7,5 por ciento y un grupo control normotenso. Se realizó ecocardiograma de superficie. Se midió activación de ROCK en leucocitos circulantes midiendo MYPT1 fosforilado/total (p/t) por Western blot y la velocidad de pulso carotídeo-femoral (PWV) para estimar distensibilidad arterial. El stress oxidativo se estimó midiendo ma-londialdehído (MDA) y 8-isoprostano (8-ISO) en suero.Resultados:
Se incluyeron 21 pacientes hipertensos con DMII, 38 pacientes hipertensos sin DMII y 34 controles normotensos. La edad promedio fue 51 +/- 0,9; 48 +/- 0,9 y 52 (p NS) +/- 1,1 y el 47 por ciento, 50 por ciento y 52 por ciento (p NS) eran mujeres respectivamente. Los pacientes HT con DMII presentaron MYPTl p/t (5,6 +/- 1,3; 3,6 +/- 0,4; 2,1 +/- 0,1 p< 0,01), MDA (1,8 +/- 0,4/ABSTRACT
Background:
Rho/Rho-kinase intracellular pathway is activated by membrane bound small G-proteins. Activation of Rho/Rho-kinase pathway is related to pathologic cardiac remodeling. We have previously observed this activation (ROCK) in hypertensive patients with left ventricular hypertrophy. The influence of diabetes in this relationship has not been explored.Aim:
to evaluate the activation of Rho-kinase and oxi-dative stress in hypertensive patients with type II diabetes (DMII).Methods:
A comparative study between patients with untreated hypertension (HT), hypertensive patients with diabetes and hemoglobin A1c > 7.5 percent and normotensi-ve control subjects was performed. LVH was assessed by echocardiography. ROCK activity was measured in peripheral leukocytes by Western blot determination of phosphorilated / total MYPT1 ratio. Arterial compliance was determined by the relationship of carotid and femoral velocity signals (PWV) Oxidative stress was estimated by serum levels of malondialdehyde (MDA) and 8-isoprostane (8-ISO).Results:
Hypertensives with DMII (n=21) had a mean age of 51 +/- 0.9 years, and 47 percent were females. Corresponding figures for 38 hypertensive patients without DM and 34 control patients were 48 ± 0,9 and 52 +/- 1,1 (NS) and 50 percent and 52 percent females, respectively (NS). The MYPT1 p/t ratio was 5,6 +/- 1,3; 3,6 +/- 0,4; 2,1 +/- 0,1 (p<0.01) in the 3 groups, respectively. MDA for the 3 groups was 1,8 +/- 0,4/Conclusion:
At comparable levels of left ventricular mass index, diabetic hypertensive patients had increased ROCK activity compared to non diabetic hypertensives. Also, compared to non-diabetic hypertensives and normotensive controls, diabetic hypertensive patients exhibited increased...
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Estresse Oxidativo
/
Quinases Associadas a rho
/
Hipertensão
Tipo de estudo:
Observational_studies
/
Prevalence_studies
/
Risk_factors_studies
Limite:
Adult
/
Female
/
Humans
/
Male
Idioma:
Es
Ano de publicação:
2011
Tipo de documento:
Article