Genetic variability in G2 and F2 region between biological clones of human respiratory syncytial virus with or without host immune selection pressure
Moraes, Claudia Trigo Pedroso; Oliveira, Danielle Bruna Leal; Campos, Angelica Cristine Almeida; Bosso, Patricia Alves; Lima, Hildener Nogueira; Stewien, Klaus Eberhard; Gilio, Alfredo Elias; Vieira, Sandra Elisabete; Botosso, Viviane Fongaro; Durigon, Edison Luiz.
Mem. Inst. Oswaldo Cruz
; 110(1): 138-141, 03/02/2015. tab
Artigo
em Inglês
| LILACS | ID: lil-741607
Human respiratory syncytial virus (HRSV) is an important respiratory pathogens among children between zero-five years old. Host immunity and viral genetic variability are important factors that can make vaccine production difficult. In this work, differences between biological clones of HRSV were detected in clinical samples in the absence and presence of serum collected from children in the convalescent phase of the illness and from their biological mothers. Viral clones were selected by plaque assay in the absence and presence of serum and nucleotide sequences of the G2 and F2 genes of HRSV biological clones were compared. One non-synonymous mutation was found in the F gene (Ile5Asn) in one clone of an HRSV-B sample and one non-synonymous mutation was found in the G gene (Ser291Pro) in four clones of the same HRSV-B sample. Only one of these clones was obtained after treatment with the child's serum. In addition, some synonymous mutations were determined in two clones of the HRSV-A samples. In conclusion, it is possible that minor sequences could be selected by host antibodies contributing to the HRSV evolutionary process, hampering the development of an effective vaccine, since we verify the same codon alteration in absence and presence of human sera in individual clones of BR-85 sample.
Assuntos
Óxido de Alumínio/química Cocos/química Produtos Agrícolas/crescimento & desenvolvimento Frutas/química Monoterpenos/análise Óleos Voláteis/química Pelargonium/crescimento & desenvolvimento Dióxido de Silício/química Produtos Agrícolas/química Produtos Agrícolas/economia Produtos Agrícolas/metabolismo Indústria de Processamento de Alimentos/economia Irã (Geográfico) Resíduos Industriais/análise Resíduos Industriais/economia Monoterpenos/metabolismo Óleos Voláteis/economia Óleos Voláteis/isolamento & purificação Óleos Voláteis/metabolismo Pelargonium/química Pelargonium/metabolismo Perfumes/química Perfumes/economia Perfumes/isolamento & purificação Perfumes/metabolismo Folhas de Planta/química Folhas de Planta/crescimento & desenvolvimento Folhas de Planta/metabolismo Silicatos/química Solo/química Terpenos/análise Terpenos/metabolismo
Biblioteca responsável:
BR1.1
powered by iAHx-2.18-89
Portal de Pesquisa da BVS