Vesiculoviruses (VSV) are zoonotic
viruses that cause
vesicular stomatitis disease in
cattle,
horses and
pigs, as well as sporadic
human cases of acute febrile illness. Therefore,
diagnosis of VSV
infections by reliable
laboratory techniques is important to allow a proper
case management and implementation of
strategies for the containment of
virus spread. We show here a sensitive and reproducible real-
time reverse transcriptase polymerase chain reaction (RT-PCR) for
detection and quantification of VSV. The assay was evaluated with
arthropods and
serum samples obtained from
horses,
cattle and
patients with acute febrile
disease. The real-
time RT-PCR amplified the Piry, Carajas, Alagoas and
Indiana Vesiculovirus at a
melting temperature 81.02 ± 0.8ºC, and the
sensitivity of assay was estimated in 10
RNA copies/mL to the Piry
Vesiculovirus. The
viral genome has been detected in samples of
horses and
cattle, but not detected in
human sera or
arthropods. Thus, this assay allows a preliminary
differential diagnosis of VSV
infections.