Technique for the detection of Toxoplasma gondii antigens in mouse urine
Mem. Inst. Oswaldo Cruz
; 85(1): 65-8, jan.-mar. 1990. tab
Article
em En
| LILACS
| ID: lil-85169
Biblioteca responsável:
BR15.1
RESUMO
A simple and rapid staphylococcal coagglutination test for the detection of Toxoplasma gondii antigens in mice urine is described. A suspension of protein-A containing Staphylococcus aureus coated with rabbit hyperimmune serum was used as reagent. The sensitivity of the antigen assay was found to be at least 118 ng of the antigen protein per ml. No coagglutination was observed when the reagent was challenged against antigenic solutions of other parasites. The suitability of the method for detecting antigens of T. gondii in urine samples was studied by experimental toxoplasma infection in mice. Before the staphylococcal test, the urine samples were double serially diluted in 0.1 M PBS. From the second day on all samples from infected mice were positive at 1/16 dilution. At this dilution, all samples from non infected mice were negative or did not produce coagglutination. This method might be used in the rapid etiological diagnosis also in human cases of acute toxoplasmosis
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Toxoplasma
/
Toxoplasmose
/
Antígenos de Protozoários
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article