Kir 4.1 channel expression in neuroblastomaxglioma hybrid NG108-15 cell line.
Brain Res Dev Brain Res
; 114(1): 127-34, 1999 Apr 12.
Article
em En
| MEDLINE
| ID: mdl-10209250
ABSTRACT
To study a possible involvement of inwardly rectifying K+ 4.1 (Kir 4. 1) channels in neural cell development, RT-PCR, immunocytochemistry and whole-cell patch-clamp techniques were used to assess expression of Kir 4.1 channels in proliferating and differentiated NG108-15 cells. RT-PCR revealed co-expression of Kir 4.1 and rat ether-a-go-go-related gene (R-ERG) mRNAs in both proliferating and differentiated cells. The relative Kir 4.1 mRNA concentration increased markedly as cells progressed from undifferentiated to differentiated cells. Kir 4.1-immunoreactivity was barely detectable in undifferentiated cells, but clearly detected in differentiated cells, indicating that Kir 4.1 gene and protein expressions are developmentally regulated. However, corresponding Kir 4.1 current could not be detected in differentiated cells using whole-cell patch-clamp recording. The 'silent' channel/receptor, often found in tumor cells, may carry genetic defects, which prevent functional expression of the channel. NG108-15 may serve as unique model for studying the relationship between the expression of an ion channel gene and the electrophysiological phenotype it encodes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Canais de Potássio
/
Regulação Neoplásica da Expressão Gênica
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Regulação da Expressão Gênica no Desenvolvimento
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Canais de Potássio Corretores do Fluxo de Internalização
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Canais de Potássio de Abertura Dependente da Tensão da Membrana
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Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article