Measurement of urinary amino acids using high-performance liquid chromatography equipped with a strong cation exchange resin pre-column.
Ann Clin Biochem
; 36 ( Pt 2): 202-6, 1999 Mar.
Article
em En
| MEDLINE
| ID: mdl-10370737
ABSTRACT
We determined the urinary amino acid concentrations by high-performance liquid chromatography (HPLC) using an eluent buffer which had been processed through a column packed with strong cation-exchange resin. With this column, ghost peaks resolved or much reduced in size and shifted to a post-arginine position. A clearer separation of peaks of urinary amino acids and ammonia was obtained. This could be due to the elimination of O-phthalaldehyde reactive substances in the eluent by the resin column. This method does not require a separate step to remove ammonia, unlike most of the methods currently used, and thus saves time.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Aminoácidos
Limite:
Humans
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article