Flow cytometric analysis of circadian changes in platelet activation using anti-GMP-140 monoclonal antibody.
Chronobiol Int
; 16(3): 335-42, 1999 May.
Article
em En
| MEDLINE
| ID: mdl-10373102
ABSTRACT
The hemostatic activity of blood shows a circadian variation with a higher frequency of acute coronary events in the morning. The thrombotic tendency of blood is influenced by many factors, including platelets. Diurnal changes of in vivo platelet activation were investigated by whole blood flow cytometry in 10 young healthy male volunteers using anti-GMP-140 (anti-alpha-granule membrane protein 140 kD) monoclonal antibody at 3h intervals from 0600 to 2400. We also studied circulating platelet aggregates to investigate whether there exists a similarity between the results of these methods. Results of flow cytometric analysis indicate that there is an increase in platelet activation during the period from 0600 to 0900. Platelet activation then decreases gradually during the period from noon to midnight. These changes are accompanied by a similar trend in circulating platelet aggregates. This suggests that GMP-140 expression on platelets is synchronized with or followed by platelet aggregate formation in vivo, and increased platelet activation may predispose individuals to thrombosis at this time.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ativação Plaquetária
/
Ritmo Circadiano
/
Selectina-P
/
Anticorpos Monoclonais
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article