Genomic organization, expression, and analysis of the troponin C gene pat-10 of Caenorhabditis elegans.
J Cell Biol
; 146(1): 193-202, 1999 Jul 12.
Article
em En
| MEDLINE
| ID: mdl-10402470
ABSTRACT
We have cloned and characterized the troponin C gene, pat-10 of the nematode Caenorhabditis elegans. At the amino acid level nematode troponin C is most similar to troponin C of Drosophila (45% identity) and cardiac troponin C of vertebrates. Expression studies demonstrate that this troponin is expressed in body wall muscle throughout the life of the animal. Later, vulval muscles and anal muscles also express this troponin C isoform. The structural gene for this troponin is pat-10 and mutations in this gene lead to animals that arrest as twofold paralyzed embryos late in development. We have sequenced two of the mutations in pat-10 and both had identical two mutations in the gene; one changes D64 to N and the other changes W153 to a termination site. The missense alteration affects a calcium-binding site and eliminates calcium binding, whereas the second mutation eliminates binding to troponin I. These combined biochemical and in vivo studies of mutant animals demonstrate that this troponin is essential for proper muscle function during development.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
/
Genes de Helmintos
/
Caenorhabditis elegans
/
Troponina C
/
Proteínas de Caenorhabditis elegans
Limite:
Animals
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article