Green fluorescent protein (GFP) as a reporter gene for the plant pathogenic oomycete Phytophthora palmivora.
FEMS Microbiol Lett
; 178(1): 71-80, 1999 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-10483725
ABSTRACT
Transgenic Phytophthora palmivora strains that produce green fluorescent protein (GFP) or beta-glucuronidase (GUS) constitutively were obtained after stable DNA integration using a polyethylene-glycol and CaCl2-based transformation protocol. GFP and GUS production were monitored during several stages of the life cycle of P. palmivora to evaluate their use in molecular and physiological studies. 40% of the GFP transformants produced the GFP to a level detectable by a confocal laser scanning microscope, whereas 75% of the GUS transformants produced GUS. GFP could be visualised readily in swimming zoospores and other developmental stages of P. palmivora cells. For high magnification microscopic studies, GFP is better visualised and was superior to GUS. In contrast, for macroscopic examination, GUS was superior. Our findings indicate that both GFP and GUS can be used successfully as reporter genes in P. palmivora.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Phytophthora
/
Plantas
/
Genes Reporter
/
Proteínas de Algas
/
Glucuronidase
/
Proteínas Luminescentes
Limite:
Animals
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article