Oxidative DNA base damage in lymphocytes of HIV-infected drug users.
Free Radic Res
; 31(3): 197-200, 1999 Sep.
Article
em En
| MEDLINE
| ID: mdl-10499776
ABSTRACT
In the present study, we have studied the level of oxidative DNA base damage in lymphocytes of HIV-infected intravenous drug users (IDUs) and a seronegative control group. Chromatin was isolated from the lymphocytes and then analyzed by gas chromatography/isotope-dilution mass spectrometry with selected-ion monitoring (GC/IDMS-SIM). Significantly greater levels of four oxidatively modified DNA bases were observed in chromatin samples from the symptomatic HIV-infected patients than in those from the seronegative patients. These were 5-hydroxyuracil, 5-hydroxycytosine, 8-hydroxyadenine and 8-hydroxyguanine. In the case of 5-hydroxyuracil and 8-hydroxyguanine, a statistically significant difference was also found between the control group and the asymptomatic HIV-positive patients. These results suggest that oxidative stress may play an important role in the pathogenesis of acquired immune deficiency syndrome (AIDS), and that administration of antioxidant drugs to HIV-infected patients may offer protection against AIDS-related carcinogenesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dano ao DNA
/
Linfócitos
/
Infecções por HIV
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Abuso de Substâncias por Via Intravenosa
/
Estresse Oxidativo
Tipo de estudo:
Clinical_trials
Limite:
Adolescent
/
Adult
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Humans
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Male
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article