Genomic organization and genetic mapping of the bovine PREF-1 gene.
Biochem Biophys Res Commun
; 264(3): 662-7, 1999 Nov 02.
Article
em En
| MEDLINE
| ID: mdl-10543989
ABSTRACT
As a potential regulator of nutrient partitioning in beef cattle, we have cloned and genetically mapped the bovine PREF-1 gene. A full-length PREF-1 cDNA was isolated by iterative purification from a mixed-tissue cDNA library to which adipose contributed mRNA. Analysis of partial cDNAs from this library revealed that the 3'-terminal exon of the bovine PREF-1 mRNA is spliced in a manner analogous to its murine ortholog. However, we have also detected a PREF-1 splice form apparently unique to cattle. Aside from this alternative selection of a splice donor in the bovine fifth exon, the exon/intron junctions of the bovine PREF-1 gene recapitulate those observed for mice. The sequences proximal to the bovine PREF-1 transcription start site are homologous to the mouse PREF-1 promoter. Importantly, the sequence experimentally identified as critical to PREF-1 "suppression in adipocyte differentiation" is conserved in the bovine gene. The bovine PREF-1 gene was mapped to the telomeric end of BTA 21 by virtue of a physically linked microsatellite with seven alleles and 285 informative meiosis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
Mapeamento Cromossômico
/
DNA Complementar
/
Proteínas de Membrana
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article