Control of the DNA damage checkpoint by chk1 and rad53 protein kinases through distinct mechanisms.
Science
; 286(5442): 1166-71, 1999 Nov 05.
Article
em En
| MEDLINE
| ID: mdl-10550056
ABSTRACT
In response to DNA damage, cells activate checkpoint pathways that prevent cell cycle progression. In fission yeast and mammals, mitotic arrest in response to DNA damage requires inhibitory Cdk phosphorylation regulated by Chk1. This study indicates that Chk1 is required for function of the DNA damage checkpoint in Saccharomyces cerevisiae but acts through a distinct mechanism maintaining the abundance of Pds1, an anaphase inhibitor. Unlike other checkpoint mutants, chk1 mutants were only mildly sensitive to DNA damage, indicating that checkpoint functions besides cell cycle arrest influence damage sensitivity. Another kinase, Rad53, was required to both maintain active cyclin-dependent kinase 1, Cdk1(Cdc28), and prevent anaphase entry after checkpoint activation. Evidence suggests that Rad53 exerts its role in checkpoint control through regulation of the Polo kinase Cdc5. These results support a model in which Chk1 and Rad53 function in parallel through Pds1 and Cdc5, respectively, to prevent anaphase entry and mitotic exit after DNA damage. This model provides a possible explanation for the role of Cdc5 in DNA damage checkpoint adaptation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Quinases
/
Saccharomyces cerevisiae
/
Dano ao DNA
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Proteínas Serina-Treonina Quinases
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Proteínas de Saccharomyces cerevisiae
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Complexos Ubiquitina-Proteína Ligase
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Mitose
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article