Induction of presynaptic differentiation in cultured neurons by extracellular matrix components.
Eur J Neurosci
; 11(10): 3457-67, 1999 Oct.
Article
em En
| MEDLINE
| ID: mdl-10564354
ABSTRACT
Motoneurons reinnervating skeletal muscles form nerve terminals at sites of contact with a specialized basal lamina. To analyse the molecules and mechanisms that underly these responses, we introduce two systems in which basal lamina-derived components induce presynaptic differentiation of cultured neurons from chick ciliary ganglia in the absence of a postsynaptic cell. In one, ciliary neurites that contact substrates coated with a recombinant laminin beta2 fragment form varicosities that are rich in synaptic vesicle proteins, depleted of neurofilaments, and capable of depolarization-dependent exocytosis and endocytosis. Thus, a single molecule can trigger a complex, coordinated program of presynaptic differentiation. In a second system, neurites growing on cryostat sections of adult kidney form vesicle-rich, neurofilament-poor arbors on glomeruli. Glomerular basal lamina, like synaptic basal lamina, is rich in laminin beta2 and collagen (alpha3-5) IV. However, glomeruli from mutant mice lacking these proteins were capable of inducing differentiation, suggesting the glomerulus as a source of novel presynaptic organizing molecules.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas da Matriz Extracelular
/
Fibras Musculares Esqueléticas
/
Neurônios Motores
Limite:
Animals
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article