Short report: evaluation of a monoclonal antibody-based latex agglutination test for rapid diagnosis of septicemic melioidosis.
Am J Trop Med Hyg
; 61(5): 735-7, 1999 Nov.
Article
em En
| MEDLINE
| ID: mdl-10586904
ABSTRACT
A monoclonal antibody (MAb)-based latex agglutination (MAb-LA) test was developed to rapidly identify Burkholderia pseudomallei in hemoculture of patients with septicemic melioidosis. The method was evaluated in a clinical situation on 396 hemocultures positive for bacterial growth, of which 75 cultures were positive for B. pseudomallei by conventional biochemical tests. The sensitivity and specificity of the MAb-LA test were 95% and 100%, respectively. The positive and negative predictive values were 100% and 99%. The method is highly reliable and suitable for rapid diagnosis of septicemic melioidosis, reducing the time normally required from a minimum of 3-4 days by conventional methods to less than 30 hr. Most of these 30 hr are involved in growing up enough bacteria to perform the MAb-LA test, which itself takes only 1 min.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Testes de Fixação do Látex
/
Burkholderia pseudomallei
/
Bacteriemia
/
Melioidose
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article