Gene transfer into hypothalamic organotypic cultures using an adeno-associated virus vector.
Exp Neurol
; 160(2): 313-6, 1999 Dec.
Article
em En
| MEDLINE
| ID: mdl-10619549
ABSTRACT
Organotypic cultures of rat hypothalamic slice cultures were successfully transduced using adeno-associated viral vectors. Using nuclear-targeted Lac-Z as the reporter gene, transduction was found to be very effective, occurring in as high as 89% of a specific cell type, the oxytocin neurons, present in the cultured explants. These transduction levels were not accompanied by any deleterious effects in the cultured cells 7 days after transduction. Such an in vitro approach should be valuable for the study of cell-specific gene expression in neurons in the central nervous system for which there are no homologous (surrogate) cell lines.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Beta-Galactosidase
/
Técnicas de Transferência de Genes
/
Dependovirus
/
Hipotálamo
/
Neurônios
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article