A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model.
Jpn J Physiol
; 50(2): 281-4, 2000 Apr.
Article
em En
| MEDLINE
| ID: mdl-10880887
ABSTRACT
Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
Apoptose
/
Fragmentação do DNA
/
Insuficiência Cardíaca
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article