Immunofluorescence and biochemical techniques to detect nuclear localization of ciliary neurotrophic factor in glial cells.
Brain Res Brain Res Protoc
; 5(3): 273-81, 2000 Jul.
Article
em En
| MEDLINE
| ID: mdl-10906493
Ciliary neurotrophic factor (CNTF) promotes the survival of several populations of neurons, including sensory and motor neurons. It is mainly produced by Schwann cells and astrocytes and exerts its biological function via a specific membrane receptor. We recently determined the nuclear localization of CNTF in producing cells, after transfection and in the heterologous system of Xenopus oocytes. In the present paper, we describe in detail the techniques for the detection of CNTF in the nucleus of rat astrocytes, transfected cells, isolated nuclei and injected Xenopus oocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transfecção
/
Núcleo Celular
/
Astrócitos
/
Técnica Indireta de Fluorescência para Anticorpo
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Fator Neurotrófico Ciliar
Limite:
Animals
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article