The spherulites(TM): a promising carrier for oligonucleotide delivery.
Nucleic Acids Res
; 28(16): 3134-42, 2000 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-10931929
ABSTRACT
Concentric multilamellar microvesicles, named spherulites(TM), were evaluated as an oligonucleotide carrier. Up to 80% oligonucleotide was encapsulated in these vesicles. The study was carried out on two different spherulite(TM) formulations. The spherulite(TM) size and stability characteristics are presented. Delivery of encapsulated oligonucleotide was performed on a rat hepatocarcinoma and on a lymphoblastoid T cell line, both expressing the luciferase gene. We showed that spherulites(TM) were able to transfect both adherent and suspension cell lines and deliver the oligonucleotide to the nucleus. Moreover, 48-62% luciferase inhibition was obtained in the rat hepatocarcinoma cell line when the antisense oligonucleotide targeted to the luciferase coding region was encapsulated at 500 nM concentration in spherulites(TM) of different compositions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Portadores de Fármacos
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Transfecção
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Oligodesoxirribonucleotídeos Antissenso
Limite:
Animals
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article