Genotyping of Bacteroides fragilis isolates from stool specimens by arbitrarily-primed-PCR.
Diagn Microbiol Infect Dis
; 37(4): 225-9, 2000 Aug.
Article
em En
| MEDLINE
| ID: mdl-10974572
ABSTRACT
In order to determine genetic relatedness of Bacteroides fragilis isolates from different clinical sources, arbitrarily primed polymerase chain reaction (PCR) (AP-PCR) was used to compare 17 strains isolated from patients with inflammatory bowel disease (IBD) and 20 strains isolated from foals with diarrhea. Three reference ATCC strains were also analyzed. Eighteen unique types were identified with a 22-mer arbitrary primer (ERIC-2) among the 20 patient isolates. Types 1 (enterotoxigenic) and 9 (nonenterotoxigenic), were each found in the stools of two patients. All other isolates showed a distinct and unique DNA banding pattern indicating a high degree of genotypic variability. Eleven types were identified among the foal isolates. Type 20, a nonenterotoxigenic type, was present in 30% of the foals. No correlation was found between the human and horse isolates. No clear relationship between a disease state (diarrhea or IBD) and specific types was observed. AP-PCR will be useful as a rapid method to determine genetic relatedness and in future epidemiologic studies of diarrheal diseases due to B. fragilis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacteroides fragilis
/
Doenças Inflamatórias Intestinais
/
Diarreia
/
Fezes
/
Doenças dos Cavalos
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article