A blaze-dry spreading procedure for the electron microscopy of chromosomes from acid alcohol-fixed human lymphocytes.
Stain Technol
; 50(4): 233-7, 1975 Jul.
Article
em En
| MEDLINE
| ID: mdl-1103372
ABSTRACT
A procedure is described for the blaze-drying of human lymphocyte chromosomes on carbonized Parlodion film. Films are prepared by applying Parlodion solution to sheets of freshly cleaved mica. Damage to the film during blaze-drying is prevented by chilling the mica sheets on dry ice before flaming. After spreading, the film and metaphases are floated free from the mica and transferred to a slide of Formav-coated electron microscope grids. The resulting preparations yield complete metaphase spreads and banded chromosomes morphologically similar to those observed with the light microscope.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos
/
Microscopia Eletrônica
/
Técnicas Citológicas
/
Cromossomos
Limite:
Humans
Idioma:
En
Ano de publicação:
1975
Tipo de documento:
Article