Linkage disequilibrium and haplotype mapping of a skin cancer susceptibility locus in outbred mice.
Mamm Genome
; 11(11): 979-81, 2000 Nov.
Article
em En
| MEDLINE
| ID: mdl-11063253
Car-R (carcinogenesis-resistant) and Car-S (carcinogenesis-susceptible) outbred mice, obtained by phenotypic selection from an initial intercross of eight inbred strains, show a >100-fold difference in their susceptibility to two-stage skin tumorigenesis. We found that the lines carry a high degree of genetic polymorphism. with an average heterozygosity of 0.39. This polymorphism allowed the use of linkage disequilibrium (LD) and haplotype analysis for the mapping of a skin cancer modifier locus on Chr 7, in a short region of 6 cM, around the Tyr gene. Car-S mice inherited the susceptibility allele at this locus from the A/J, BALB/c, SJL/J, and SWR/J strains. Our results demonstrate the feasibility and usefulness of mapping disease genes by LD in phenotypically selected, genetically heterogeneous animals.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Cutâneas
/
Haplótipos
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Desequilíbrio de Ligação
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Animais não Endogâmicos
Limite:
Animals
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article