Influence of insulin treatment and feed restriction on follicular development in cyclic gilts.

ABSTRACT

Crossbred gilts were used to investigate whether exogenous insulin can restore normal follicular growth in feed-restricted gilts. After an 18-day altrenogest treatment, the first day of oestrous behaviour was designed as day 0. From day 0 to 13, all gilts received the same amount of feed, calculated to meet 200% of the energy requirements for maintenance. On day 14, luteolysis was induced by injection of an analogue of prostaglandin F2alpha. All gilts were slaughtered on day 19 and their ovaries removed. In Experiment 1, gilts received a high (240% of maintenance) or low (80%) level of feeding (n=10/group) from day 14 to 18. The number of large follicles (> or = 5 mm) on day 19 was reduced in feed-restricted gilts (16.9 versus 20.6, P<0.05). The same protocol of feed restriction was used in Experiment 2 (240% versus 80% of maintenance from day 14 to 18), and some gilts received daily injections of insulin (0.6 IU live weight kg(-1)). The three experimental groups were H 240% and no insulin (n=8); H-I 240%+insulin (n=8) and L-I 80%+insulin (n=7). On day 18, 4 h after insulin injection, plasma insulin was higher in insulin-treated than in untreated gilts and glucose concentrations were reduced more dramatically in L-I than in H-I gilts (P<0.05). Concentrations of IGF-I were lower in L-I than in other gilts (P<0.05) and plasma IGFBPs were not significantly affected by treatments. On day 19, the number of large follicles (> or = 5 mm) was not significantly influenced by treatments (19.4, 17.6 and 15.3 for H, H-I and L-I gilts, respectively). Insulin, IGF-I and IGFBP-2 levels in follicular fluids from large follicles did not differ between females whereas IGFBP-3 levels were lower in L-I than in H gilts (P<0.05) and intermediate in H-I gilts. Intrafollicular levels of glucose were higher in feed-restricted than in well-fed gilts (P<0.05). These results suggest that exogenous insulin does not restore final follicular growth impaired by acute undernutrition.