PCR detection of human enteric viruses in bathing areas, waste waters and human stools in Southwestern France.
Res Microbiol
; 151(8): 693-701, 2000 Oct.
Article
em En
| MEDLINE
| ID: mdl-11081583
ABSTRACT
Detection of human pathogenic viruses by molecular techniques might be suitable for identifying viral pollution in environmental waters and for improving diagnosis in patients. Environmental samples were taken from bathing areas and sewage treatment plants in southwestern France. Small volume samples (50 microL) were tested. Five groups of enteric pathogenic viruses were studied enteroviruses, Norwalk-like viruses (NLVs), hepatitis A virus, rotaviruses and adenoviruses. Moreover, human samples were tested for NLV. After extraction of viral nucleic acids (Boom's procedure), a nested polymerase chain reaction was conducted before hybridization. Five bathing waters out of 26 were positive for one viral group, without systematic association with bacterial contamination. Eight sewage plant samples out of 13 were positive for at least one viral group. Seven patients out of 45 were NLV-positive. Molecular techniques allow efficient screening of viral contamination in environmental waters and the study of NLV molecular epidemiology.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esgotos
/
Vírus
/
Microbiologia da Água
/
Reação em Cadeia da Polimerase
/
Fezes
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Risk_factors_studies
Limite:
Humans
País/Região como assunto:
Europa
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article