The structure of the carbohydrate backbone of the core-lipid A region of the lipopolysaccharide from Proteus vulgaris serotype O25.

ABSTRACT

The following structure of the lipid A-core region of the lipopolysaccharide (LPS) from Proteus vulgaris serotype O25 was determined by using NMR and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of LPS, of the products of alkaline deacylation of the LPS, and of the products of LPS deamination [structure see text] Terminal residues of beta-GlcNAc and beta-Kdo (indicated by bold italics) are present alternatively in approximately 32 amount, leaving no unsubstituted beta-Gal. All sugars are in the pyranose form, alpha-Hep is the residue of L-glycero-alpha-D-manno-Hep, alpha-DDHep is the residue of D-glycero-alpha-D-manno-Hep.