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Searching for the physiological function of 17beta-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus: studies of substrate specificity and expression analysis.
Lanisnik Rizner, T; Stojan, J; Adamski, J.
Afiliação
  • Lanisnik Rizner T; Institute of Biochemistry, Medical Faculty, University of Ljubljana, Vrazov trg 2, 1000, Ljubljana, Slovenia. Lanisnik@ibmi.mf.uni-lj.si
Mol Cell Endocrinol ; 171(1-2): 193-8, 2001 Jan 22.
Article em En | MEDLINE | ID: mdl-11165029
17beta-hydroxysteroid dehydrogenase from the filamentous fungus Cochliobolus lunatus (17beta-HSDcl) has recently been characterized. Since its function is still unclear, we performed substrate specificity studies to obtain some indications about its physiological function. Different steroids were studied as putative substrates of recombinant 17beta-HSDcl, androgens and estrogens, brassinosteroids, and the fungal steroid herbarulid. Among these androgens and estrogens were most efficiently converted. The following substrates in decreasing order were best reduced: 4-estrene-3,17-dione, 5alpha-androstane-3,17-dione, 4-androstene-3,17-dione and estrone. Two typical inhibitors were tested: carbenoxolone--a representative inhibitor of the SDR family and quercetin--a diagnostic inhibitor of carbonyl reductases. Among these two quercetin was more efficient. Expression studies revealed that 17beta-HSDcl is mainly expressed in the stationary phase of growth indicating its possible involvement in secondary metabolism.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ascomicetos / Proteínas Fúngicas / Expressão Gênica / Etiocolanolona / 17-Hidroxiesteroide Desidrogenases Idioma: En Ano de publicação: 2001 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ascomicetos / Proteínas Fúngicas / Expressão Gênica / Etiocolanolona / 17-Hidroxiesteroide Desidrogenases Idioma: En Ano de publicação: 2001 Tipo de documento: Article