New broad-host-range promoter probe vectors based on the plasmid RK2 replicon.
FEMS Microbiol Lett
; 195(1): 91-6, 2001 Feb 05.
Article
em En
| MEDLINE
| ID: mdl-11167001
ABSTRACT
Broad-host-range plasmid RK2-based promoter probe vectors with a known nucleotide sequence were constructed. In the absence of an upstream promoter, the expression of two tested reporter genes (luc and lacZ) in Escherichia coli was virtually zero, while insertion of the Ptrc promoter resulted in strong inducer-dependent expression. The lacZ-based vectors were mobilized into Pseudomonas fluorescens ST, Pseudomonas putida KT2442, Sphingomonas spp. and Burkholderia spp. LB400, and expression analyses indicated that the properties observed in E. coli are maintained across the species barriers. In addition, the previously established knowledge of RK2 molecular biology allows easy manipulations of features such as plasmid copy number, further extending the application potential of the vectors.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Replicon
/
Regiões Promotoras Genéticas
/
Vetores Genéticos
/
Bactérias Gram-Negativas
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article