Modulation of cell cycle-related protein expression by sodium butyrate in human non-small cell lung cancer cell lines.
Int J Cancer
; 91(5): 654-7, 2001 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-11267976
ABSTRACT
To elucidate the mechanism of action of sodium butyrate (NaB), we examined its effect on the expression of some cell cycle-related proteins (cyclins D1 and E, p16(ink4), p21(waf1), p27(kip1)) in 2 human non-small cell lung cancer cell lines (NCI-460 and NCI-H23) characterized by wild- type and mutant TP53, respectively. The growth of both cell lines was inhibited in a dose-dependent manner and this process was accompanied by a modulation of cell cycle-related proteins. In NCI-H460, the p27(kip1) and p16(ink4) protein levels were markedly increased following NaB treatment, whereas p21(waf1) was only slightly elevated, with a peak at 2 mM NaB, and p53 was unaffected by any concentration. By contrast, in NCI-H23, a marked increase in p21(waf1) protein was paralleled by decreased p53 levels, whereas all the other investigated proteins remained stable. The results suggest that NaB blocks the growth of both cell lines by induction of cyclin-dependent kinase inhibitors (in particular, p21(waf1) in NCI-H23 and p27(kip1) and p16(ink4) in NCI-H460) through a p53-dependent or p53-independent mechanism, and open up interesting perspectives for the use of NaB as an alternative or additional strategy in the treatment of non-small cell lung carcinoma.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sódio
/
Butiratos
/
Ciclo Celular
/
Carcinoma Pulmonar de Células não Pequenas
/
Proteínas de Ciclo Celular
/
Proteínas Supressoras de Tumor
/
Neoplasias Pulmonares
Limite:
Humans
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article