Genotoxic effects of butadiene in mouse lung cells detected by an ex vivo micronucleus test.
Mutat Res
; 491(1-2): 81-5, 2001 Apr 05.
Article
em En
| MEDLINE
| ID: mdl-11287301
ABSTRACT
Lung fibroblasts from BD-exposed mice have been analysed for the occurrence of micronuclei. Primary cultures set up 24h after the end of exposure were treated with cytochalasin B and micronuclei scored in binucleate cells. A three-fold statistically significant increase of micronucleated cells was detected after exposure to 500ppm, the lowest tested concentration. A linear dose effect relationship was observed between 500 and 1300ppm. Immunofluorescent staining of kinetochore proteins was applied to distinguish between acentric micronuclei produced by chromosome breaks and micronuclei containing a centromeric region, most likely induced by chromosome loss. A statistically significant increase of both types of MN in 1300ppm-exposed females and a significant increase in centromeric MN in 500ppm-exposed males were detected. These data demonstrate that an intermediate of BD metabolism with a potential for clastogenic and aneugenic effects is active in lung cells after inhalation exposure. These effects can play a role in the initiation and promotion of BD-induced lung tumours.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Butadienos
/
Pulmão
/
Mutagênicos
Limite:
Animals
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article