X-rays induce dose-dependent and cell cycle-independent accumulation of p21(sdi1/WAF1).
Hiroshima J Med Sci
; 50(1): 1-7, 2001 Mar.
Article
em En
| MEDLINE
| ID: mdl-11314855
ABSTRACT
Cell cycle arrest at the G1 checkpoint is governed by a function of wild-type p53. We assessed the behavior of the sdi1 gene, which codes for a 21kDa potent inhibitor of cdk/cyclins, after X-irradiation. X-irradiation induced sdi1 mRNA accumulation and G1 arrest only in cells possessing wild-type p53. Elevation of p21(sdi1/WAF1) was preceded by p53 accumulation, which occurred despite p53 mRNA constancy in normal cells growing in the log phase. The quantity of accumulated p53 and p21(sdi1/WAF1) was radiation dose dependent. A decrease in the S phase cell population in normal cells observed after irradiation reached a minimum at less-than-maximum levels of p53 and p21(sdi1/WAF1). Furthermore, an accumulation of p53 and p21(sdi1/WAF1) was also observed when cells were synchronized in the G0, G1 and S phase and X-irradiated. These results indicated that an X-ray induced p53 and p21(sdi1/WAF1) accumulation mechanism exists throughout the cell cycle, and that the signal strength induced by X-irradiation is dose-dependent.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ciclinas
/
Fibroblastos
Limite:
Humans
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article