Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains.

Park, S J; Ahn, W S; Green, P R; Lee, S Y

Park, S J; Ahn, W S; Green, P R; Lee, S Y.

Afiliação

Park SJ; Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea.

Biotechnol Bioeng ; 74(1): 81-6, 2001 Jul 05.

Article em En | MEDLINE | ID: mdl-11353413

ABSTRACT

ABSTRACT

The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) [P(3HB-co-3HV-co-3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer.

Assuntos

Escherichia coli/genética; Polímeros/metabolismo; Aciltransferases/genética; Aciltransferases/metabolismo; Aeromonas/genética; Enoil-CoA Hidratase/genética; Enoil-CoA Hidratase/metabolismo; Escherichia coli/metabolismo; Fermentação; Engenharia Genética; Ácidos Láuricos/metabolismo

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polímeros / Escherichia coli Idioma: En Ano de publicação: 2001 Tipo de documento: Article

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PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polímeros / Escherichia coli Idioma: En Ano de publicação: 2001 Tipo de documento: Article