A peptidorhamnomannan from the mycelium of Pseudallescheria boydii is a potential diagnostic antigen of this emerging human pathogen.
Microbiology (Reading)
; 147(Pt 6): 1499-1506, 2001 Jun.
Article
em En
| MEDLINE
| ID: mdl-11390680
ABSTRACT
The ascomycete Pseudallescheria boydii is an emerging human pathogen frequently found in soil and polluted water. A peptidopolysaccharide antigen has been isolated from mycelial forms of P. boydii, and characterized using chemical and immunological methods. Monosaccharide composition, methylation analysis, and (1)H- and (13)C-NMR spectra indicated the presence of a rhamnomannan with a structure distinct from those of similar components isolated from other fungi, containing Rhap(1-->3)Rhap epitopes on side chains which may be linked (1-->3) to (1-->6)-linked mannose. The peptidorhamnomannan from P. boydii reacted poorly with an antiserum raised against whole cells of Sporothrix schenckii and strongly with one against P. boydii hyphae. These characteristics and immunological differences suggest that this major rhamnose-containing antigen of P. boydii may be useful for the specific diagnosis of infections attributable to this fungus.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pseudallescheria
/
Glicoproteínas
/
Antígenos de Fungos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article