Diagnostic utility of polymerase chain reaction and immunohistochemical techniques for the laboratory diagnosis of intracranial tuberculoma.
Clin Neuropathol
; 20(4): 176-80, 2001.
Article
em En
| MEDLINE
| ID: mdl-11495007
ABSTRACT
In an attempt to establish a tuberculous etiology, polymerase chain reaction (PCR) and immunohistochemical (IHC) methods were undertaken in formalin-fixed paraffin sections of ten surgical specimens of intracranial tuberculoma. The control group included an equal number of intracranial fungal granuloma. Both PCR and IHC methods did not yield false-positive results in fungal granuloma. PCR was found to be less sensitive (60%) than IHC method (80%) in this study. IHC method definitely possesses several operational advantages over PCR and is more suited to laboratories in developing countries for establishing a tuberculous etiology particularly in those patients in whom the conventional bacteriological methods did not confirm the diagnosis of tuberculoma.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tuberculoma
/
Encefalopatias
/
Imuno-Histoquímica
/
Reação em Cadeia da Polimerase
/
Técnicas de Laboratório Clínico
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Risk_factors_studies
Limite:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article