Ruminococcin A, a new lantibiotic produced by a Ruminococcus gnavus strain isolated from human feces.
Appl Environ Microbiol
; 67(9): 4111-8, 2001 Sep.
Article
em En
| MEDLINE
| ID: mdl-11526013
ABSTRACT
When cultivated in the presence of trypsin, the Ruminococcus gnavus E1 strain, isolated from a human fecal sample, was able to produce an antibacterial substance that accumulated in the supernatant. This substance, called ruminococcin A, was purified to homogeneity by reverse-phase chromatography. It was shown to be a 2,675-Da bacteriocin harboring a lanthionine structure. The utilization of Edman degradation and tandem mass spectrometry techniques, followed by DNA sequencing of part of the structural gene, allowed the identification of 21 amino acid residues. Similarity to other bacteriocins present in sequence libraries strongly suggested that ruminococcin A belonged to class IIA of the lantibiotics. The purified ruminococcin A was active against various pathogenic clostridia and bacteria phylogenetically related to R. gnavus. This is the first report on the characterization of a bacteriocin produced by a strictly anaerobic bacterium from human fecal microbiota.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacteriocinas
/
Cocos Gram-Positivos
/
Fezes
Limite:
Humans
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article