Influence of buoyancy-driven convection on protein separation by free-flow electrophoresis.
Adv Space Res
; 12(1): 373-83, 1992.
Article
em En
| MEDLINE
| ID: mdl-11536983
ABSTRACT
In free-flow electrophoresis, the stability and reproducibility of the flow field are prerequisites for a satisfactory separation. Due to the presence of the species to be separated and due to mass- and heat-transfer phenomena, non-uniformities in density inevitably appear, both within the carrier buffer and between the carrier buffer and the sample stream. On earth they give rise to buoyancy-driven convection which interferes with the separation. These effects have been quantified by numerical modelling. Agreement has been found between the numerical results and experimental observations.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Convecção
/
Eletroforese
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article