In vitro transactivation of Bacillus subtilis RNase P RNA.
FEBS Lett
; 506(3): 235-8, 2001 Oct 12.
Article
em En
| MEDLINE
| ID: mdl-11602252
ABSTRACT
Deletion of the 'signature' PL5.1 stem-loop structure of a Type II RNase P RNA diminished its catalytic activity. Addition of PL5.1 in trans increased catalytic efficiency (kcat/KM) rather than kcat. Transactivation was due to the binding of a single PL5.1 species per ribozyme with an apparent Kd near 600 nM. The results are consistent with the role of PL5.1 being to position the substrate near the active site of the ribozyme, and with the hypothesis that ribozymes can evolve by accretion of preformed smaller structures.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
/
RNA Bacteriano
/
Ativação Transcricional
/
RNA Catalítico
/
Endorribonucleases
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article